help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Jong, P. M.
Right arrow Articles by Ponec, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Jong, P. M.
Right arrow Articles by Ponec, M.

Am. J. Respir. Cell Mol. Biol., Vol 10, No. 3, Mar 1994, 271-277.

Ciliogenesis in human bronchial epithelial cells cultured at the air- liquid interface

PM de Jong, MA van Sterkenburg, SC Hesseling, JA Kempenaar, AA Mulder, AM Mommaas, JH Dijkman and M Ponec
Department of Pulmonology, University Hospital, Leiden, the Netherlands.

We present a study on modification of culture conditions in serially cultured human bronchial epithelial cells (HBEC), necessary to achieve bronchial epithelial cells similar to the native epithelium. Cells were obtained from bronchial biopsies and serially cultured using a previously described method (In Vitro Cell. Dev. Biol. 1993; 29A:379- 387). At the air-liquid interface, the second and the subsequent passages of HBEC cultures were grown 7 to 31 days, in medium containing fetal calf serum, using de-epidermized dermis or collagen discs as substratum. Scanning and transmission electron microscopy revealed ciliogenesis after 7 days and maturation of the cilia up to 31 days, irrespective of whether de-epidermized dermis or collagen membrane was used. The transmission electron microscopy of the developing cilia showed fibrogranular masses, procentrioles, basal bodies, and in the mature cilia a normal ultrastructure of the axoneme, the nine doublets, the central pair, radial spokes, and dynein arms in the ciliary shaft. In contrast, the submerged cultures showed no signs of ciliogenesis in the same time course. Results of experiments, in which cell seeding density, the substrate used, and the manner of nutrient supplementation were modulated, revealed that the air-exposure of the cultured HBEC is a necessary requirement for the ciliogenesis. The development pathway of ciliated cells in air-exposed HBEC cultures was similar to the differentiation and maturation pattern in human fetal tracheal cells. The in vitro model of human bronchial epithelial cells derived from biopsies obtained by fiberoptic bronchoscopy offers an attractive model for future studies on the function of human bronchial epithelial cells under normal and pathologic conditions.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. A. Tomei, M. M. Choe, and M. A. Swartz
Effects of dynamic compression on lentiviral transduction in an in vitro airway wall model
Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L79 - L86.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. J. Ross, L. A. Dailey, L. E. Brighton, and R. B. Devlin
Transcriptional Profiling of Mucociliary Differentiation in Human Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., August 1, 2007; 37(2): 169 - 185.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. Wiszniewski, L. Jornot, T. Dudez, A. Pagano, T. Rochat, J. S. Lacroix, S. Suter, and M. Chanson
Long-Term Cultures of Polarized Airway Epithelial Cells from Patients with Cystic Fibrosis
Am. J. Respir. Cell Mol. Biol., January 1, 2006; 34(1): 39 - 48.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
I. A. Forrest, D. M. Murphy, C. Ward, D. Jones, G. E. Johnson, L. Archer, F. K. Gould, T. E. Cawston, J. L. Lordan, and P. A. Corris
Primary airway epithelial cell culture from lung transplant recipients
Eur. Respir. J., December 1, 2005; 26(6): 1080 - 1085.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. You, E. J. Richer, T. Huang, and S. L. Brody
Growth and differentiation of mouse tracheal epithelial cells: selection of a proliferative population
Am J Physiol Lung Cell Mol Physiol, December 1, 2002; 283(6): L1315 - L1321.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
R. C. Lantz, R. Lemus, R. W. Lange, and M. H. Karol
Rapid Reduction of Intracellular Glutathione in Human Bronchial Epithelial Cells Exposed to Occupational Levels of Toluene Diisocyanate
Toxicol. Sci., April 1, 2001; 60(2): 348 - 355.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A Delplanque, C Coraux, R Tirouvanziam, I Khazaal, E Puchelle, P Ambros, D Gaillard, and B Peault
Epithelial stem cell-mediated development of the human respiratory mucosa in SCID mice
J. Cell Sci., January 3, 2000; 113(5): 767 - 778.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
K Million, J Larcher, J Laoukili, D Bourguignon, F Marano, and F Tournier
Polyglutamylation and polyglycylation of alpha- and beta-tubulins during in vitro ciliated cell differentiation of human respiratory epithelial cells
J. Cell Sci., January 12, 1999; 112(23): 4357 - 4366.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. LeDizet, J. C. Beck, and W. E. Finkbeiner
Differential regulation of centrin genes during ciliogenesis in human tracheal epithelial cells
Am J Physiol Lung Cell Mol Physiol, December 1, 1998; 275(6): L1145 - L1156.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1994 American Thoracic Society.