Am. J. Respir. Cell Mol. Biol., Vol 10, No. 4, 04 1994, 378-383.
Effect of furosemide on prostaglandin synthesis by human nasal and bronchial epithelial cells in culture
GM Levasseur-Acker, M Molimard, J Regnard, E Naline, C Freche and A Lockhart
Laboratoire de Physiologie Respiratoire, Faculte de Medecine Cochin Port-Royal, Paris, France.
Inhaled furosemide protects asthmatic subjects against bronchial
obstruction caused by indirect provocants. We have attempted to correlate
the protective effect of furosemide with its ability to alter prostaglandin
(PG) synthesis by the airway epithelium. Human epithelial cells from nasal
polyps and bronchi were cultured in DME-Ham's F12 medium with 10% fetal
calf serum. Confluent cells (days 6 through 8) were incubated for 30 min in
fresh medium, and the PGs in the supernatant were measured by
radioimmunoassay. Spontaneous output (ng.ml-1.mg-1 cell protein) was as
follows (mean +/- SEM): PGE2 = 7.74 +/- 2.10 (n = 12), PGF2 alpha = 1.66
+/- 0.12 (n = 15), 6-keto-PGF1 alpha = 4.32 +/- 1.37 (n = 11), PGD2 = 0.73
+/- 0.16 (n = 11) for bronchial cells and PGE2 = 7.24 +/- 0.80 (n = 32),
PGF2 alpha = 1.38 +/- 0.12 (n = 17), 6-keto-PGF1 alpha = 6.79 +/- 2.50 (n =
15), PGD2 = 0.42 +/- 0.07 (n = 17) for nasal cells. Incubation with
arachidonic acid (25 micrograms/ml) for 30 min significantly increased the
amounts of the four PGs. Incubation with furosemide (10(-4) M) for 30 min
caused a marked reduction in both basal and arachidonic acid-stimulated
production of PGE2 and PGF2 alpha but did not reduce production of 6-
keto-PGF1 alpha and PGD2. Incubation with bumetanide (10(-4) M) for 30 min
did not modify the PGE2 synthesis by nasal epithelial cells.(ABSTRACT
TRUNCATED AT 250 WORDS)
