help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marsh, C. B.
Right arrow Articles by Wewers, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marsh, C. B.
Right arrow Articles by Wewers, M. D.

Am. J. Respir. Cell Mol. Biol., Vol 10, No. 5, 05 1994, 521-525.

Cytokine-induced interleukin-1 receptor antagonist release in mononuclear phagocytes

CB Marsh and MD Wewers
Division of Pulmonary and Critical Care Medicine, Ohio State University, Columbus.

IL-1ra has recently been shown to suppress both cytokine- and endotoxin- induced IL-1 beta and TNF-alpha release from monocytes. Given that mononuclear phagocytes can produce both the proinflammatory cytokines IL-1 alpha, IL-1 beta, and TNF-alpha as well as the suppressive cytokine IL-1ra, we proposed that IL-1 alpha, IL-1 beta, and TNF-alpha may induce IL-1ra from mononuclear phagocytes. To test this hypothesis, human mononuclear cells were stimulated for 18 h with IL-1 alpha, IL-1 beta, or TNF-alpha, and the supernatants assayed for IL-1ra by ELISA. Each cytokine induced IL-1ra secretion in a dose-response manner. However, IL-1 alpha and IL-1 beta were better inducers of IL-1ra than was TNF-alpha. IL-1 alpha or IL-1 beta at a dose of 10 ng/ml induced 3 to 6 ng/ml of IL-1ra, while TNF-alpha at a dose of 100 ng/ml stimulated only 1.4 ng/ml of IL-1ra. This induction was not due to endotoxin, as all cytokines contained less than 10 pg/ml of contaminating LPS. Furthermore, for IL-1 beta-induced IL-1ra, immunoprecipitation of IL-1 beta with an anti-IL-1 beta antibody, but not a preimmune antibody, blocked the induction of IL-1ra. In contrast to mononuclear phagocytes, IL-1 alpha, IL-1 beta, and TNF-alpha did not induce further IL-1ra production in alveolar macrophages. This lack of macrophage responsiveness may relate to the constitutive production of IL-1ra by these mature mononuclear phagocytes.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 ChestHome page
J. A. Dosman, A. Senthilselvan, S. P. Kirychuk, S. Lemay, E. M. Barber, P. Willson, Y. Cormier, and T. S. Hurst
Positive Human Health Effects of Wearing a Respirator in a Swine Barn
Chest, September 1, 2000; 118(3): 852 - 860.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. Kol, B. W. Donesky, K. Ruutiainen-Altman, I. Ben-Shlomo, M. Irahara, M. Ando, R. M. Rohan, and E. Y. Adashi
Ovarian Interleukin-1 Receptor Antagonist in Rats: Gene Expression, Cellular Localization, Cyclic Variation, and Hormonal Regulation of a Potential Determinant of Interleukin-1 Action
Biol Reprod, July 1, 1999; 61(1): 274 - 282.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. R. Coulter, M. D. Wewers, M. P. Lowe, and D. L. Knoell
Extracellular Regulation of Interleukin (IL)-1beta through Lung Epithelial Cells and Defective IL-1 Type II Receptor Expression
Am. J. Respir. Cell Mol. Biol., May 1, 1999; 20(5): 964 - 975.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. SENTHILSELVAN, Y. ZHANG, J. A. DOSMAN, E. M. BARBER, L. E. HOLFELD, S. P. KIRYCHUK, Y. CORMIER, T. S. HURST, and C. S. RHODES
Positive Human Health Effects of Dust Suppression with Canola Oil in Swine Barns
Am. J. Respir. Crit. Care Med., July 1, 1997; 156(2): 410 - 417.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1994 American Thoracic Society.