help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hwang, H. C.
Right arrow Articles by Albelda, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hwang, H. C.
Right arrow Articles by Albelda, S. M.

Am. J. Respir. Cell Mol. Biol., Vol 13, No. 1, 07 1995, 7-16.

Gene therapy using adenovirus carrying the herpes simplex-thymidine kinase gene to treat in vivo models of human malignant mesothelioma and lung cancer

HC Hwang, WR Smythe, AA Elshami, JC Kucharczuk, KM Amin, JP Williams, LA Litzky, LR Kaiser and SM Albelda
Department of Medicine; University of Pennsylvania Medical Center, Philadelphia, USA.

Previous studies have shown adenoviral transfer of the herpes simplex virus thymidine kinase (HSVtk) gene followed by the anti-viral drug ganciclovir (GCV) can be used to successfully treat established human mesothelioma tumors growing within the peritoneal cavities of severe combined immune deficient (SCID) mice. These findings raised a number of questions important to the applicability, efficiency, and safety of this treatment strategy. In this report, we have further characterized the use of recombinant adenovirus carrying the HSVtk gene to treat mesothelioma and other localized malignancies. Our results indicate that the Ad.RSVtk/GCV system is effective in causing tumor regression in animals inoculated with another mesothelioma cell line and a lung cancer cell line and that animals with bulky disease can be successfully treated. Effects are seen at a wide range of virus doses and significant anti-tumor activity is present at doses of ganciclovir that are clinically achievable. Finally, this treatment approach appears safe, with limited dissemination of virus using a sensitive RT- PCR detection system. These studies further characterize the use of adenoviral transfer of the HSVtk gene to treat experimental mesothelioma and suggest that clinical trials using this approach may be feasible.


This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
D. H. Sterman, A. Recio, A. Vachani, J. Sun, L. Cheung, P. DeLong, K. M. Amin, L. A. Litzky, J. M. Wilson, L. R. Kaiser, et al.
Long-term Follow-up of Patients with Malignant Pleural Mesothelioma Receiving High-Dose Adenovirus Herpes Simplex Thymidine Kinase/Ganciclovir Suicide Gene Therapy
Clin. Cancer Res., October 15, 2005; 11(20): 7444 - 7453.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
M. Fukunaga, S. Takamori, A. Hayashi, K. Shirouzu, and K.-i. Kosai
Adenoviral herpes simplex virus thymidine kinase gene therapy in an orthotopic lung cancer model
Ann. Thorac. Surg., June 1, 2002; 73(6): 1740 - 1746.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
R. Kurdow, A. S. Boehle, S. Haye, L. Boenicke, B. Schniewind, P. Dohrmann, and H. Kalthoff
Ganciclovir prodrug therapy is effective in a murine xenotransplant model of human lung cancer
Ann. Thorac. Surg., March 1, 2002; 73(3): 905 - 910.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
R. Zhang, F. H. Straus, and L. J. DeGroot
Cell-Specific Viral Gene Therapy of a Hurthle Cell Tumor
J. Clin. Endocrinol. Metab., March 1, 2002; 87(3): 1407 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Kawauchi, J.-i. Suzuki, R. Morishita, Y. Wada, A. Izawa, N. Tomita, J. Amano, Y. Kaneda, T. Ogihara, S. Takamoto, et al.
Gene Therapy for Attenuating Cardiac Allograft Arteriopathy Using Ex Vivo E2F Decoy Transfection by HVJ-AVE-Liposome Method in Mice and Nonhuman Primates
Circ. Res., November 24, 2000; 87(11): 1063 - 1068.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
P. P. Claudio, M. Caputi, and A. Giordano
The RB2/p130 Gene: The Latest Weapon in the War against Lung Cancer?
Clin. Cancer Res., March 1, 2000; 6(3): 754 - 764.
[Abstract] [Full Text]

Home page
 ChestHome page
D. H. Sterman, L. R. Kaiser, and S. M. Albelda
Advances in the Treatment of Malignant Pleural Mesothelioma
Chest, August 1, 1999; 116(2): 504 - 520.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
R. Lee, C. H.R. Boasquevisque, M. M. Boglione, M. Hiratsuka, R. K. Scheule, J. D. Cooper, and G. A. Patterson
Isolated lung liposome-mediated gene transfer produces organ-specific transgenic expression
Ann. Thorac. Surg., September 1, 1998; 66(3): 903 - 907.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. Schwarzenberger, D. Lei, S. M. Freeman, P. Ye, A. Weinacker, C. Theodossiou, W. Summer, and J. K. Kolls
Antitumor Activity with the HSV-tk-gene-modified Cell Line PA-1-STK in Malignant Mesothelioma
Am. J. Respir. Cell Mol. Biol., August 1, 1998; 19(2): 333 - 337.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. K. Batra, J. C. Olsen, R. J. Pickles, D. K. Hoganson, and R. C. Boucher
Transduction of Non-Small Cell Lung Cancer Cells by Adenoviral and Retroviral Vectors
Am. J. Respir. Cell Mol. Biol., March 1, 1998; 18(3): 402 - 410.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. S. Strayer, S. H. Guttentag, and P. L. Ballard
Targeting Type II and Clara Cells for Adenovirus-mediated Gene Transfer Using the Surfactant Protein B Promoter
Am. J. Respir. Cell Mol. Biol., January 1, 1998; 18(1): 1 - 9.
[Abstract] [Full Text]

Home page
 Ann. Thorac. Surg.Home page
D. B. Campbell
Malignant Mesothelioma
Ann. Thorac. Surg., May 1, 1997; 63(5): 1503 - 1505.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1995 American Thoracic Society.