Am. J. Respir. Cell Mol. Biol., Vol 15, No. 4, Oct 1996, 451-459.
Quantitation of the alveolar distribution of surfactant mixtures in normal and injured lungs
G Pataki, L Czopf, BA Holm and S Matalon
Department of Anesthesiology, University of Alabama at Birmingham 35233- 1924, USA.
The uptake and distribution of two surfactant mixtures, Exosurf and
Infasurf, instilled into the lungs of normal and hyperoxia-exposed (100%
O2, 60 h) rabbits, were quantified at the alveolar level using flow
cytometry and fluorescence microscopy. The surfactants were labeled with
the fluorescent phospholipid analog NBD-C12-PC (1-
palmitoyl-2-[12-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)amino]dodecanoyl]-
sn-glycero-3-phosphocholine ) in 100:1 molar ratio. Rabbits were killed 2 h
after the instillation of either surfactant (80 mg phospholipid [PL]/kg),
and alveolar macrophages (AM) and alveolar type II (ATII) cells were
isolated and examined for the presence of NBD fluorescence. The fractions
of cells, with NBD fluorescence values higher than autofluorescence,
isolated from the lungs of air-breathing rabbits instilled with either
Infasurf or Exosurf, were 84% and 63% for AM, and 55% and 45% for ATII
cells. Exposure of rabbits to hyperoxia decreased the fraction of
NBD-positive AM following Infasurf instillation, and the mean increase in
NBD-associated fluorescence in ATII cells following Exosurf instillation.
Our results suggest that sublethal hyperoxia decreases the short-term
uptake but not the distribution of intratracheally instilled Exosurf.
