J Atsuta, SA Sterbinsky, J Plitt, LM Schwiebert, BS Bochner and RP Schleimer
Johns Hopkins Asthma and Allergy Center, Baltimore, Maryland 21224- 6801, USA.

Airway epithelium may actively participate in inflammatory responses, such as occur in asthma. The presence and regulation of surface molecules on the airway epithelium, however, is incompletely understood. We have determined the phenotype of the human bronchial epithelial cell line BEAS-2B by flow cytometry. We confirmed previous observations that human bronchial epithelial cells constitutively express CD29, CD44, CD49a, CD49b, CD49c, CD49d, CD49e, CD49f, CD51, CD54 (ICAM-1), CD61, and HLA class 1. BEAS-2B cells were also found to constitutively express CD9, CD13, CD15, CD15s, CD23, CD33, CD36, CD40, CD41b, CD42b, CD48, CD50, CD71, and CD102 (ICAM-2). Culture of BEAS-2B cells with tumor necrosis factor (TNF)-alpha or interleukin (IL)-1beta (1 ng/ml) was found to enhance intercellular adhesion molecule-1 (ICAM- 1) expression (several fold) and induce de novo CD106 [vascular cell adhesion molecule-1 (VCAM-1)] expression. TNF-alpha or IL-1beta did not change the expression of CD9, CD13, CD16, CD23, CD29, CD31, CD32, CD35, CD45, CD61, or CD64 in BEAS-2B cells. IL-4 (1 ng/ml) also induced expression of VCAM-1 (1.5-fold) but not ICAM- expression while interferon-gamma (1 ng/ml) enhanced only ICAM-1 expression (2-fold). Maximal VCAM-1 expression was obtained with the combination of TNF- alpha and IL-4 (8-fold). Using Northern blot hybridization analysis, ICAM-1 and VCAM-1 mRNA was detected in BEAS-2B cells stimulated with cytokines. VCAM-1 on stimulated BEAS-2B was functionally active as determined by adhesion of purified eosinophils and blockade with specific antibodies. Primary isolates of bronchial epithelial cells produced detectable levels of VCAM-1 protein and mRNA as detected by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction, respectively. These results suggest that cytokine activation induces expression of ICAM-1 and VCAM-1 on airway epithelium, an event which may influence leukocyte infiltration and activation.

This article has been cited by other articles:

				G. R. Bolduc and L. C. Madoff The group B streptococcal alpha C protein binds {alpha}1 1-integrin through a novel KTD motif that promotes internalization of GBS within human epithelial cells Microbiology, December 1, 2007; 153(12): 4039 - 4049. [Abstract] [Full Text] [PDF]

				C.-Y. A. Chen, Y. Yamashita, T.-C. Chang, A. Yamashita, W. Zhu, Z. Zhong, and A.-B. Shyu Versatile applications of transcriptional pulsing to study mRNA turnover in mammalian cells RNA, October 1, 2007; 13(10): 1775 - 1786. [Abstract] [Full Text] [PDF]

				N. Regamey, C. Obregon, S. Ferrari-Lacraz, C. van Leer, M. Chanson, L. P. Nicod, and T. Geiser Airway Epithelial IL-15 Transforms Monocytes into Dendritic Cells Am. J. Respir. Cell Mol. Biol., July 1, 2007; 37(1): 75 - 84. [Abstract] [Full Text] [PDF]

				L. Farkas, M.-C. Hahn, M. Schmoczer, N. Jentsch, K. Kratzel, M. Pfeifer, and C. Schulz Expression of CXC Chemokine Receptors 1 and 2 in Human Bronchial Epithelial Cells Chest, November 1, 2005; 128(5): 3724 - 3734. [Abstract] [Full Text] [PDF]

				J. Kim, A. C. Myers, L. Chen, D. M. Pardoll, Q.-A. Truong-Tran, A. P. Lane, J. F. McDyer, L. Fortuno, and R. P. Schleimer Constitutive and Inducible Expression of B7 Family of Ligands by Human Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., September 1, 2005; 33(3): 280 - 289. [Abstract] [Full Text] [PDF]

				M. E. Poynter, R. Cloots, T. van Woerkom, K. J. Butnor, P. Vacek, D. J. Taatjes, C. G. Irvin, and Y. M. W. Janssen-Heininger NF-{kappa}B Activation in Airways Modulates Allergic Inflammation but Not Hyperresponsiveness J. Immunol., December 1, 2004; 173(11): 7003 - 7009. [Abstract] [Full Text] [PDF]

				R. P. Schleimer Glucocorticoids Suppress Inflammation but Spare Innate Immune Responses in Airway Epithelium Proceedings of the ATS, November 1, 2004; 1(3): 222 - 230. [Abstract] [Full Text] [PDF]

				C. E. Chwieralski, I. Schnurra, L. Thim, and W. Hoffmann Epidermal Growth Factor and Trefoil Factor Family 2 Synergistically Trigger Chemotaxis on BEAS-2B Cells via Different Signaling Cascades Am. J. Respir. Cell Mol. Biol., November 1, 2004; 31(5): 528 - 537. [Abstract] [Full Text] [PDF]

				M. I. Wickremasinghe, L. H. Thomas, C. M. O'Kane, J. Uddin, and J. S. Friedland Transcriptional Mechanisms Regulating Alveolar Epithelial Cell-specific CCL5 Secretion in Pulmonary Tuberculosis J. Biol. Chem., June 25, 2004; 279(26): 27199 - 27210. [Abstract] [Full Text] [PDF]

				F. Cagnoni, S. Oddera, J. Giron-Michel, A. M. Riccio, S. Olsson, P. Dellacasa, G. Melioli, G. W. Canonica, and B. Azzarone CD40 on Adult Human Airway Epithelial Cells: Expression and Proinflammatory Effects J. Immunol., March 1, 2004; 172(5): 3205 - 3214. [Abstract] [Full Text] [PDF]

				S. Kurosawa, A. C. Myers, L. Chen, S. Wang, J. Ni, J. R. Plitt, N. M. Heller, B. S. Bochner, and R. P. Schleimer Expression of the Costimulatory Molecule B7-H2 (Inducible Costimulator Ligand) by Human Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., May 1, 2003; 28(5): 563 - 573. [Abstract] [Full Text] [PDF]

				J. C. Kim, D. Whitaker-Menezes, M. Deguchi, B. S. Adair, R. Korngold, and G. F. Murphy Novel Expression of Vascular Cell Adhesion Molecule-1 (CD106) by Squamous Epithelium in Experimental Acute Graft-versus-Host Disease Am. J. Pathol., September 1, 2002; 161(3): 763 - 770. [Abstract] [Full Text] [PDF]

				K. Wolf, C. Schulz, G.A.J. Riegger, and M. Pfeifer Tumour necrosis factor-{alpha} induced CD70 and interleukin-7R mRNA expression in BEAS-2B cells Eur. Respir. J., August 1, 2002; 20(2): 369 - 375. [Abstract] [Full Text] [PDF]

				D.-H. Nahm, Y. E. Lee, E.-J. Yim, H.-S. Park, H. Yim, Y. Kang, and J.-K. Kim Identification of Cytokeratin 18 as a Bronchial Epithelial Autoantigen Associated with Nonallergic Asthma Am. J. Respir. Crit. Care Med., June 1, 2002; 165(11): 1536 - 1539. [Abstract] [Full Text] [PDF]

				A. Burke-Gaffney, K. Blease, A. Hartnell, and P. G. Hellewell TNF-{alpha} Potentiates C5a-Stimulated Eosinophil Adhesion to Human Bronchial Epithelial Cells: A Role for {alpha}5{beta}1 Integrin J. Immunol., February 1, 2002; 168(3): 1380 - 1388. [Abstract] [Full Text] [PDF]

				B. Beck-Schimmer, R. C. Schimmer, C. Madjdpour, J. M. Bonvini, T. Pasch, and P. A. Ward Hypoxia Mediates Increased Neutrophil and Macrophage Adhesiveness to Alveolar Epithelial Cells Am. J. Respir. Cell Mol. Biol., December 1, 2001; 25(6): 780 - 787. [Abstract] [Full Text] [PDF]

				H. Nakamura, A. D. Luster, H. Tateno, S. Jedrzkiewicz, G. Tamura, K. J. Haley, E. A. Garcia-Zepeda, K. Yamaguchi, and C. M. Lilly IL-4 differentially regulates eotaxin and MCP-4 in lung epithelium and circulating mononuclear cells Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1288 - L1302. [Abstract] [Full Text] [PDF]

				R. L. Robson, R. M. McLoughlin, J. Witowski, P. Loetscher, T. S. Wilkinson, S. A. Jones, and N. Topley Differential Regulation of Chemokine Production in Human Peritoneal Mesothelial Cells: IFN-{{gamma}} Controls Neutrophil Migration Across the Mesothelium In Vitro and In Vivo J. Immunol., July 15, 2001; 167(2): 1028 - 1038. [Abstract] [Full Text] [PDF]

				R. K. Mehlotra, L. R. Hall, M. A. Haxhiu, and E. Pearlman Reciprocal Immunomodulatory Effects of Gamma Interferon and Interleukin-4 on Filaria-Induced Airway Hyperresponsiveness Infect. Immun., March 1, 2001; 69(3): 1463 - 1468. [Abstract] [Full Text] [PDF]

				S. M. Propst, R. Denson, E. Rothstein, K. Estell, and L. M. Schwiebert Proinflammatory and Th2-Derived Cytokines Modulate CD40-Mediated Expression of Inflammatory Mediators in Airway Epithelia: Implications for the Role of Epithelial CD40 in Airway Inflammation J. Immunol., August 15, 2000; 165(4): 2214 - 2221. [Abstract] [Full Text] [PDF]

				D. Sanmugalingam, A. J. Wardlaw, and P. Bradding Adhesion of human lung mast cells to bronchial epithelium: evidence for a novel carbohydrate-mediated mechanism J. Leukoc. Biol., July 1, 2000; 68(1): 38 - 46. [Abstract] [Full Text]

				A. Papi and S. L. Johnston Respiratory Epithelial Cell Expression of Vascular Cell Adhesion Molecule-1 and Its Up-regulation by Rhinovirus Infection via NF-kappa B and GATA Transcription Factors J. Biol. Chem., October 15, 1999; 274(42): 30041 - 30051. [Abstract] [Full Text] [PDF]

				E. Salik, M. Tyorkin, S. Mohan, I. George, K. Becker, E. Oei, T. Kalb, and K. Sperber Antigen Trafficking and Accessory Cell Function in Respiratory Epithelial Cells Am. J. Respir. Cell Mol. Biol., September 1, 1999; 21(3): 365 - 379. [Abstract] [Full Text]

				A. Celi, S. Cianchetti, S. Petruzzelli, S. Carnevali, F. Baliva, and C. Giuntini ICAM-1-independent adhesion of neutrophils to phorbol ester-stimulated human airway epithelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 1999; 277(3): L465 - L471. [Abstract] [Full Text] [PDF]

				F. Bühling, A. Gerber, C. Häckel, S. Krüger, T. Köhnlein, D. Brömme, D. Reinhold, S. Ansorge, and T. Welte Expression of Cathepsin K in Lung Epithelial Cells Am. J. Respir. Cell Mol. Biol., April 1, 1999; 20(4): 612 - 619. [Abstract] [Full Text]

				J. Atsuta, J. Plitt, B. S. Bochner, and R. P. Schleimer Inhibition of VCAM-1 Expression in Human Bronchial Epithelial Cells by Glucocorticoids Am. J. Respir. Cell Mol. Biol., April 1, 1999; 20(4): 643 - 650. [Abstract] [Full Text]

				A. Sauty, M. Dziejman, R. A. Taha, A. S. Iarossi, K. Neote, E. A. Garcia-Zepeda, Q. Hamid, and A. D. Luster The T Cell-Specific CXC Chemokines IP-10, Mig, and I-TAC Are Expressed by Activated Human Bronchial Epithelial Cells J. Immunol., March 15, 1999; 162(6): 3549 - 3558. [Abstract] [Full Text] [PDF]

				J. A. Wiley and A. G. Harmsen Bone Marrow-Derived Cells Are Required for the Induction of a Pulmonary Inflammatory Response Mediated by CD40 Ligation Am. J. Pathol., March 1, 1999; 154(3): 919 - 926. [Abstract] [Full Text] [PDF]

				A. Burke-Gaffney and P. G. Hellewell A CD18/ICAM-1-dependent Pathway Mediates Eosinophil Adhesion to Human Bronchial Epithelial Cells Am. J. Respir. Cell Mol. Biol., September 1, 1998; 19(3): 408 - 418. [Abstract] [Full Text]