help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garlisi, C. G.
Right arrow Articles by Umland, S. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garlisi, C. G.
Right arrow Articles by Umland, S. P.

Am. J. Respir. Cell Mol. Biol., Vol 17, No. 5, Nov 1997, 642-651.

Airway eosinophils, T cells, Th2-type cytokine mRNA, and hyperreactivity in response to aerosol challenge of allergic mice with previously established pulmonary inflammation

CG Garlisi, A Falcone, JA Hey, TM Paster, X Fernandez, CA Rizzo, M Minnicozzi, H Jones, MM Billah, RW Egan and SP Umland
Allergy and Immunology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033-0539, USA.

Asthma is characterized by acute episodes of nonspecific airway hyperreactivity and chronic pulmonary inflammation exacerbated by stimuli including allergen exposure. In order to reproduce the physiologic and immunologic responses that occur in asthmatic patients, we have characterized a model of antigen-induced inflammation in which allergic mice (B6D2F1) that had been challenged once with aerosolized ovalbumin and had developed a pulmonary cellular infiltrate were rechallenged 1 wk later. Pulmonary inflammation in rechallenged mice was substantially greater than that in single-challenged mice. Eosinophils and activated-memory T cells (CD44+, CD45RBlo) in bronchoalveolar lavage (BAL) fluid accumulated to higher levels and with faster kinetics in response to the second challenge than in response to the first challenge. Eosinophils in lung tissue also accumulated to higher levels but with similar kinetics in response to the second challenge than in response to the first challenge. Similarly, interleukin (IL)-4 and IL-5 steady-state mRNA levels in lung tissue increased after the second challenge and were higher than those measured after a single challenge. Furthermore, treatment of mice with an anti-IL-5 monoclonal antibody 2 h prior to rechallenge inhibited antigen induced eosinophil accumulation in the lungs. In mice challenged twice, peak in vivo bronchoconstrictor responsiveness to acetylcholine was increased following the second challenge compared with that observed following the initial challenge. In contrast, ex vivo tracheal smooth muscle contractile responsiveness to acetylcholine was not altered. Although mucus accumulation and epithelial damage in pulmonary tissue were evident in mice challenged twice, these parameters were slightly reduced compared with those seen at similar times in mice challenged once. Therefore, although these mice exhibit only slight bronchial epithelial damage, the presence of significant inflammation and airway hyperreactivity to acetylcholine as well as slightly increased baseline reactivity demonstrate important similarities with the pathophysiology of asthma.


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
J. R. Crosby, M. Guha, D. Tung, D. A. Miller, B. Bender, T. P. Condon, C. York-DeFalco, R. S. Geary, B. P. Monia, J. G. Karras, et al.
Inhaled CD86 Antisense Oligonucleotide Suppresses Pulmonary Inflammation and Airway Hyper-Responsiveness in Allergic Mice
J. Pharmacol. Exp. Ther., June 1, 2007; 321(3): 938 - 946.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
V. Dolgachev, M. Thomas, A. Berlin, and N. W. Lukacs
Stem cell factor-mediated activation pathways promote murine eosinophil CCL6 production and survival
J. Leukoc. Biol., April 1, 2007; 81(4): 1111 - 1119.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. A. Birrell, E. Hardaker, S. Wong, K. McCluskie, M. Catley, J. De Alba, R. Newton, S. Haj-Yahia, K. T. Pun, C. J. Watts, et al.
I{kappa}-B Kinase-2 Inhibitor Blocks Inflammation in Human Airway Smooth Muscle and a Rat Model of Asthma
Am. J. Respir. Crit. Care Med., October 15, 2005; 172(8): 962 - 971.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. A. Berlin and N. W. Lukacs
Treatment of Cockroach Allergen Asthma Model with Imatinib Attenuates Airway Responses
Am. J. Respir. Crit. Care Med., January 1, 2005; 171(1): 35 - 39.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. R. Van Scott, J. L. Hooker, D. Ehrmann, Y. Shibata, C. Kukoly, K. Salleng, G. Westergaard, A. Sandrasagra, and J. Nyce
Dust mite-induced asthma in cynomolgus monkeys
J Appl Physiol, April 1, 2004; 96(4): 1433 - 1444.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E.-B. Haddad, S. L. Underwood, D. Dabrowski, M. A. Birrell, K. McCluskie, C. H. Battram, M. Pecoraro, M. L. Foster, and M. G. Belvisi
Critical Role for T Cells in Sephadex-Induced Airway Inflammation: Pharmacological and Immunological Characterization and Molecular Biomarker Identification
J. Immunol., March 15, 2002; 168(6): 3004 - 3016.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. T. Chapman, L. E. Otterbein, J. A. Elias, and A. M. K. Choi
Carbon monoxide attenuates aeroallergen-induced inflammation in mice
Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L209 - L216.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. TOMKINSON, G. CIESLEWICZ, C. DUEZ, K. A. LARSON, J. J. LEE, and E. W. GELFAND
Temporal Association between Airway Hyperresponsiveness and Airway Eosinophilia in Ovalbumin-Sensitized Mice
Am. J. Respir. Crit. Care Med., March 1, 2001; 163(3): 721 - 730.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. P. Justice, Y. Shibata, S. Sur, J. Mustafa, M. Fan, and M. R. Van Scott
IL-10 gene knockout attenuates allergen-induced airway hyperresponsiveness in C57BL/6 mice
Am J Physiol Lung Cell Mol Physiol, February 1, 2001; 280(2): L363 - L368.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Kawasaki, H. Takizawa, H. Yoneyama, T. Nakayama, R. Fujisawa, M. Izumizaki, T. Imai, O. Yoshie, I. Homma, K. Yamamoto, et al.
Intervention of Thymus and Activation-Regulated Chemokine Attenuates the Development of Allergic Airway Inflammation and Hyperresponsiveness in Mice
J. Immunol., February 1, 2001; 166(3): 2055 - 2062.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Blease, B. Mehrad, T. J. Standiford, N. W. Lukacs, S. L. Kunkel, S. W. Chensue, B. Lu, C. J. Gerard, and C. M. Hogaboam
Airway Remodeling Is Absent in CCR1-/- Mice During Chronic Fungal Allergic Airway Disease
J. Immunol., August 1, 2000; 165(3): 1564 - 1572.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. I. Zuberi, J. R. Apgar, S.-S. Chen, and F.-T. Liu
Role for IgE in Airway Secretions: IgE Immune Complexes Are More Potent Inducers Than Antigen Alone of Airway Inflammation in a Murine Model
J. Immunol., March 1, 2000; 164(5): 2667 - 2673.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. E. Chin, C. A. Hatfield, G. E. Winterrowd, R. F. Krzesicki, K. L. Shull, S. F. Fidler, K. P. Kolbasa, J. R. Brashler, R. L. Griffin, W. E. Fleming, et al.
Preclinical Evaluation of Anti-inflammatory Activities of the Novel Pyrrolopyrimidine PNU-142731A, a Potential Treatment for Asthma
J. Pharmacol. Exp. Ther., July 1, 1999; 290(1): 188 - 195.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. MATHUR, K. HERRMANN, X. LI, Y. QIN, J. WEINSTOCK, D. ELLIOTT, J. MONAHAN, and P. PADRID
TRFK-5 Reverses Established Airway Eosinophilia But Not Established Hyperresponsiveness in a Murine Model of Chronic Asthma
Am. J. Respir. Crit. Care Med., February 1, 1999; 159(2): 580 - 587.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. Stelts, R. W. Egan, A. Falcone, C. G. Garlisi, G. J. Gleich, W. Kreutner, T. T. Kung, D. K. Nahrebne, R. W. Chapman, and M. Minnicozzi
Eosinophils Retain Their Granule Major Basic Protein in a Murine Model of Allergic Pulmonary Inflammation
Am. J. Respir. Cell Mol. Biol., April 1, 1998; 18(4): 463 - 470.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. P. Justice, J. Crosby, M. T. Borchers, A. Tomkinson, J. J. Lee, and N. A. Lee
CD4+ T cell-dependent airway mucus production occurs in response to IL-5 expression in lung
Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L1066 - L1074.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1997 American Thoracic Society.