Am. J. Respir. Cell Mol. Biol., Volume 18, Number 1, January, 1998 111-119

Connexin 26 Expression in Human and Ferret Airways and Lung During Development

Johnny L. Carson, William Reed, Billie M. Moats-Staats, Luisa E. Brighton, Todd M. Gambling, Shih-chin Hu, and Albert M. Collier

The Departments of Pediatrics and Cell Biology and Anatomy, The Center for Environmental Medicine and Lung Biology, and Frank Porter Graham Child Development Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Coordinated microscopic and molecular biological studies were used to document gap junction expression during postnatal development in ferret tracheal epithelium and lung and in fetal and adult human airway and lung. Expression of connexin 26 (Cx26) in the ferret airways was limited to the epithelial layer and was observed only during the newborn interval. In contrast, we found Cx26 expressed in the alveolar epithelium of the ferret lung by in situ hybridization, Northern blotting, RT-PCR amplification, and immunocytochemical labeling at all ages examined. This finding was further confirmed by documentation of gap junctional plaques upon ultrastructural examination of freeze-fracture replicas of adult ferret lung tissue. Parallel studies of developing human fetal lung and airway suggested connexin expression in the airways only in the first trimester but, as in the ferret, persistent expression was observed in both fetal and adult lung. These studies suggest that the transient expression of Cx26 is a reliable early indicator of airway epithelial development and differentiation in the airways. In contrast, Cx26 expression persists throughout life in the lung, suggesting that gap junctions serve more perennial intercellular communication functions in the peripheral lung.

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