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Am. J. Respir. Cell Mol. Biol., Volume 18, Number 3, March, 1998 315-322

Human Eosinophils Express Bcl-2 Family Proteins: Modulation of Mcl-1 Expression by IFN-gamma

Anne Druilhe, Michel Arock, Liliane Le Goff, and Marina Pretolani

Unité de Pharmacologie Cellulaire, Institut Pasteur; and Centre National de la Recherche Scientifique Unité de Recherche Associéc No. 625, Centre Hospitalier Universitaire Pitié-Salpétrière, Paris, France

The expression of the Bcl-2 family proteins Bax, Mcl-1, Bcl-2, and Bcl-xL, was examined in human peripheral blood eosinophils or in umbilical-cord-blood-derived eosinophils. Immunoblot analysis disclosed high amounts of the proapoptotic factor Bax in freshly purified eosinophils of both types. Although cord-blood-derived eosinophils expressed easily detectable levels of Mcl-1, Bcl-2, and Bcl-xL, only traces or no expression of these three antiapoptotic proteins were found in peripheral blood eosinophils. Incubation of both eosinophil types for 1 to 3 days in a cytokine-deprived medium led to apoptosis, without changes in the expression of Bax, Mcl-1, Bcl-2, or Bcl-xL. Although addition of interleukin-5 or interferon-gamma (IFN-gamma ) to the culture medium increased the survival of both eosinophil types, a rise in the levels of Mcl-1 was observed only in IFN-gamma -treated cord-blood eosinophils. Together, these results indicate that human eosinophils have a specific profile of Bcl-2-family protein expression that depends on their maturation status and may be modulated by stimuli that influence their survival.




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