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Am. J. Respir. Cell Mol. Biol., Volume 18, Number 5, May, 1998 643-652

Quantitative Analysis of mRNA Encoding MUC1, MUC2, and MUC5AC Genes: A Correlation between Specific Mucin Gene Expression and Sialomucin Expression in Non-Small Cell Lung Cancer

Chong-Jen Yu, Jin-Yuh Shew, Chia-Tung Shun, Han-Tuzoo Lin, Sow-Hsong Kuo, Kwen-Tay Luh, and Pan-Chyr Yang

Departments of Internal Medicine, Pathology, and Laboratory Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University; Department of Biochemistry, College of Medicine, National Taiwan University; and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China

The expression of mucins is important for tumor invasiveness and metastasis. In our previous report (Am. J. Respir. Crit. Care Med. 1997; 155:1419-1427), non-small cell lung cancers bearing sialomucin expression tended to relapse earlier than those without sialomucin. However, it remained unclear whether the expression of sialomucin in lung cancer is caused by an abnormal glycosylation process or by the expression of a specific mucin gene product. To address this problem, we established a modified quantitative competitive polymerase chain reaction (QC-PCR) analysis. RNA internal standards of MUC1, MUC2, and MUC5AC non-tandem repeat sequences were constructed, and known copy numbers of mucin RNA internal standards were introduced into reverse transcription-polymerase chain reactions (RT-PCR) for each mucin gene in order to compete with native mucin gene RNA during the reaction. The RNA of Gbeta -like gene (a housekeeping gene) was used as internal control for the RNA analysis. Twenty-five lung cancer tissues (13 adenocarcinomas and 12 squamous cell carcinomas) were used for analysis. Mann-Whitney rank sum test was applied to compare the expression amounts of different mucin genes in tissues. The results revealed that adenocarcinoma expressed higher amounts of MUC5AC gene than did squamous cell carcinoma (P = 0.03). The expression amount of MUC5AC correlated positively with the expression status of sialomucin (P = 0.012). Further studies are anticipated to elucidate the underlying mechanism contributing to this phenomenon.




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