help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ramakrishnan, M.
Right arrow Articles by Hershenson, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramakrishnan, M.
Right arrow Articles by Hershenson, M. B.

Am. J. Respir. Cell Mol. Biol., Volume 18, Number 6, June, 1998 736-740

RAPID COMMUNICATION
Catalytic Activation of Extracellular Signal-regulated Kinases Induces Cyclin D1 Expression in Primary Tracheal Myocytes

Meera Ramakrishnan, Ndidiamaka L. Musa, Jing Li, Pai T. Liu, Richard G. Pestell, and Marc B. Hershenson

Department of Pediatrics, University of Chicago, Chicago, Illinois; and the Albert Einstein Cancer Center, Department of Medicine and Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York

We have demonstrated that extracellular signal-regulated kinases (ERKs) and cyclin D1 are required for bovine tracheal myocyte DNA synthesis. We hypothesized that catalytic activation by ERKs may regulate cyclin D1 expression in these cells. To test this hypothesis, we examined the effects of two inhibitors of ERKs and two reagents that increase the level of activated ERKs on cyclin D1 protein abundance and promoter activity. ERK activity was inhibited either by PD98059, a synthetic inhibitor of mitogen-activated protein kinase (MAPK)/ERK kinase (MEK), the upstream signaling intermediate required and sufficient for ERK activation, or by transient transfection with a dominant-negative mutant of MEK1 (MEK-2A). The level of activated ERKs was increased by transient transfection with either a constitutively active form of MEK1 (MEK-2E) or wild-type ERK2 (MAPKwt). Cyclin D1 expression was assessed either by immunoblot or cotransfection with the full-length cyclin D1 promoter subcloned into a luciferase reporter. We found that pretreatment of bovine tracheal myocytes with PD98059 significantly attenuated platelet- derived growth factor (PDGF)-induced cyclin D1 protein abundance. Furthermore, transfection with MEK-2A reduced PDGF-induced cyclin D1 promoter activity. Finally, transfection with either MEK-2E or MAPKwt induced cyclin D1 promoter activity in the absence of growth factor treatment. We conclude that catalytic activation of ERKs regulates cyclin D1 expression in airway smooth-muscle cells.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. N. Kang, J. A. Jude, R. A. Panettieri Jr., T. F. Walseth, and M. S. Kannan
Glucocorticoid regulation of CD38 expression in human airway smooth muscle cells: role of dual specificity phosphatase 1
Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L186 - L193.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K.-K. Ho, A. A. Anderson, E. Rosivatz, E. W.-F. Lam, R. Woscholski, and D. J. Mann
Identification of Cyclin A2 as the Downstream Effector of the Nuclear Phosphatidylinositol 4,5-Bisphosphate Signaling Network
J. Biol. Chem., February 29, 2008; 283(9): 5477 - 5485.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
J. K. Bentley and M. B. Hershenson
Airway Smooth Muscle Growth in Asthma: Proliferation, Hypertrophy, and Migration
Proceedings of the ATS, January 1, 2008; 5(1): 89 - 96.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Xie, M. B. Sukkar, R. Issa, N. M. Khorasani, and K. F. Chung
Mechanisms of induction of airway smooth muscle hyperplasia by transforming growth factor-beta
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L245 - L253.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. G. Tirumurugaan, J. A. Jude, B. N. Kang, R. A. Panettieri, T. F. Walseth, and M. S. Kannan
TNF-{alpha} induced CD38 expression in human airway smooth muscle cells: role of MAP kinases and transcription factors NF-{kappa}B and AP-1
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1385 - L1395.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Osawa, D. Xu, D. Sternberg, J. R. Sonett, J. D'Armiento, R. A. Panettieri, and C. W. Emala
Functional expression of the GABAB receptor in human airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L923 - L931.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Mizuno, M. Kadowaki, Y. Demura, S. Ameshima, I. Miyamori, and T. Ishizaki
p42/44 Mitogen-Activated Protein Kinase Regulated by p53 and Nitric Oxide in Human Pulmonary Arterial Smooth Muscle Cells
Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2): 184 - 192.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. Zhou, A. Tan, S. Iasvovskaia, J. Li, A. Lin, and M. B. Hershenson
Ras and Mitogen-Activated Protein Kinase Kinase Kinase-1 Coregulate Activator Protein-1- and Nuclear Factor-{kappa}B-Mediated Gene Expression in Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., June 1, 2003; 28(6): 762 - 769.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Page, J. Li, L. Zhou, S. Iasvoyskaia, K. C. Corbit, J.-W. Soh, I. B. Weinstein, A. R. Brasier, A. Lin, and M. B. Hershenson
Regulation of Airway Epithelial Cell NF-{kappa}B-Dependent Gene Expression by Protein Kinase C{delta}
J. Immunol., June 1, 2003; 170(11): 5681 - 5689.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Li, X. D. Johnson, S. Iazvovskaia, A. Tan, A. Lin, and M. B. Hershenson
Signaling intermediates required for NF-kappa B activation and IL-8 expression in CF bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L307 - L315.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Li, S. Kartha, S. Iasvovskaia, A. Tan, R. K. Bhat, J. M. Manaligod, K. Page, A. R. Brasier, and M. B. Hershenson
Regulation of human airway epithelial cell IL-8 expression by MAP kinases
Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L690 - L699.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. Page, J. Li, K. C. Corbit, K. M. Rumilla, J.-W. Soh, I. B. Weinstein, C. Albanese, R. G. Pestell, M. R. Rosner, and M. B. Hershenson
Regulation of Airway Smooth Muscle Cyclin D1 Transcription by Protein Kinase C-{delta}
Am. J. Respir. Cell Mol. Biol., August 1, 2002; 27(2): 204 - 213.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. J. Ammit and R. A. Panettieri Jr.
Signal Transduction in Smooth Muscle: Invited Review: The circle of life: cell cycle regulation in airway smooth muscle
J Appl Physiol, September 1, 2001; 91(3): 1431 - 1437.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Page, J. Li, and M. B. Hershenson
p38 MAP kinase negatively regulates cyclin D1 expression in airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L955 - L964.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. P. Bauerfeld, M. B. Hershenson, and K. Page
Cdc42, but not RhoA, regulates cyclin D1 expression in bovine tracheal myocytes
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L974 - L982.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J.-H. Lee, P. R. A. Johnson, M. Roth, N. H. Hunt, and J. L. Black
ERK activation and mitogenesis in human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L1019 - L1029.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. Page, J. Li, Y. Wang, S. Kartha, R. G. Pestell, and M. B. Hershenson
Regulation of Cyclin D1 Expression and DNA Synthesis by Phosphatidylinositol 3-Kinase in Airway Smooth Muscle Cells
Am. J. Respir. Cell Mol. Biol., October 1, 2000; 23(4): 436 - 443.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
V. P. Krymskaya, M. J. Orsini, A. J. Eszterhas, K. C. Brodbeck, J. L. Benovic, R. A. Panettieri Jr., and R. B. Penn
Mechanisms of Proliferation Synergy by Receptor Tyrosine Kinase and G Protein-Coupled Receptor Activation in Human Airway Smooth Muscle
Am. J. Respir. Cell Mol. Biol., October 1, 2000; 23(4): 546 - 554.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. B. Hershenson and M. K. Abe
Mitogen-Activated Signaling in Airway Smooth Muscle . A Central Role for Ras
Am. J. Respir. Cell Mol. Biol., December 1, 1999; 21(6): 651 - 654.
[Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
E. T. NAURECKAS, I. MAURICE NDUKWU, A. J. HALAYKO, C. MAXWELL, M. B. HERSHENSON, and J. SOLWAY
Bronchoalveolar Lavage Fluid from Asthmatic Subjects Is Mitogenic for Human Airway Smooth Muscle
Am. J. Respir. Crit. Care Med., December 1, 1999; 160(6): 2062 - 2066.
[Abstract] [Full Text]

Home page
 Mol. Pharmacol.Home page
A. G. Stewart, T. Harris, D. J. Fernandes, L. C. Schachte, V. Koutsoubos, E. Guida, C. E. Ravenhall, P. Vadiveloo, and J. W. Wilson
beta 2-Adrenergic Receptor Agonists and cAMP Arrest Human Cultured Airway Smooth Muscle Cells in the G1 Phase of the Cell Cycle: Role of Proteasome Degradation of Cyclin D1
Mol. Pharmacol., November 1, 1999; 56(5): 1079 - 1086.
[Abstract] [Full Text]

Home page
 Endocr. Rev.Home page
R. G. Pestell, C. Albanese, A. T. Reutens, J. E. Segall, R. J. Lee, and A. Arnold
The Cyclins and Cyclin-Dependent Kinase Inhibitors in Hormonal Regulation of Proliferation and Differentiation
Endocr. Rev., August 1, 1999; 20(4): 501 - 534.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Page, J. Li, J. A. Hodge, P. T. Liu, T. L. Vanden Hoek, L. B. Becker, R. G. Pestell, M. R. Rosner, and M. B. Hershenson
Characterization of a Rac1 Signaling Pathway to Cyclin D1 Expression in Airway Smooth Muscle Cells
J. Biol. Chem., July 30, 1999; 274(31): 22065 - 22071.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. Page, J. Li, and M. B. Hershenson
Platelet-Derived Growth Factor Stimulation of Mitogen-Activated Protein Kinases and Cyclin D1 Promoter Activity in Cultured Airway Smooth-Muscle Cells . Role of Ras
Am. J. Respir. Cell Mol. Biol., June 1, 1999; 20(6): 1294 - 1302.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
R. J. Lee, C. Albanese, R. J. Stenger, G. Watanabe, G. Inghirami, G. K. Haines III, M. Webster, W. J. Muller, J. S. Brugge, R. J. Davis, et al.
pp60v-src Induction of Cyclin D1 Requires Collaborative Interactions between the Extracellular Signal-regulated Kinase, p38, and Jun Kinase Pathways. A ROLE FOR cAMP RESPONSE ELEMENT-BINDING PROTEIN AND ACTIVATING TRANSCRIPTION FACTOR-2 IN pp60v-src SIGNALING IN BREAST CANCER CELLS
J. Biol. Chem., March 12, 1999; 274(11): 7341 - 7350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Glassford, M. Holman, L. Banerji, E. Clayton, G. G. B. Klaus, M. Turner, and E. W.-F. Lam
Vav Is Required for Cyclin D2 Induction and Proliferation of Mouse B Lymphocytes Activated via the Antigen Receptor
J. Biol. Chem., October 26, 2001; 276(44): 41040 - 41048.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1998 American Thoracic Society.