help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Minamiya, Y.
Right arrow Articles by Ogawa, J.-i.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Minamiya, Y.
Right arrow Articles by Ogawa, J.-i.

Am. J. Respir. Cell Mol. Biol., Volume 19, Number 1, July, 1998 150-157

Platelet-activating Factor Mediates Intercellular Adhesion Molecule-1-dependent Radical Production in the Nonhypoxic Ischemia Rat Lung

Yoshihiro Minamiya, Kasumi Tozawa, Michihiko Kitamura, Satoshi Saito, and Jun-ichi Ogawa

Second Department of Surgery, Akita University School of Medicine, Akita City, Japan

It has been reported that reperfusion is the most important factor in ischemia-reperfusion (I/R) injury. However, causes of I/R injury in the lung are controversial, because oxygen is always supplied if ventilation continues. Therefore, we hypothesized that nonhypoxic ischemia without reperfusion is sufficient for lung injury. To test our hypothesis, we measured both hydrogen peroxide (H2O2) production in the pulmonary circulation, by digital imaging fluorescent dichlorofluorescein, and microvascular permeability (MVP), by the Evans blue extravasation technique in the nonhypoxic ischemia rat lung. We made a nonhypoxic ischemia rat lung by clamping the left pulmonary artery. Both H2O2 production and MVP increased in the nonhypoxic ischemia rat lung. We also determined the effect of oxygen removal by clamping the bronchus in advance of pulmonary artery occlusion, intercellular adhesion molecule-1 (ICAM-1) neutralization with monoclonal antibody 1A29, and platelet-activating factor (PAF) receptor antagonist CV6209 on H2O2 production and MVP. These treatments inhibited both H2O2 production and MVP increase. At high-power viewing of the fluorescent dichlorofluorescein image, H2O2 was detected in the leukocytes within pulmonary capillaries. These data indicate that the nonhypoxic ischemia without reperfusion alone causes radical production and increases MVP. Furthermore, PAF and ICAM-1 contribute to these reactions.




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
W. M. Kuebler, K. Parthasarathi, J. Lindert, and J. Bhattacharya
Real-time lung microscopy
J Appl Physiol, March 1, 2007; 102(3): 1255 - 1264.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. de Perrot, M. Liu, T. K. Waddell, and S. Keshavjee
Ischemia-Reperfusion-induced Lung Injury
Am. J. Respir. Crit. Care Med., February 15, 2003; 167(4): 490 - 511.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y.-T. Lu, P.-G. Chen, and S. F. Liu
Time course of lung ischemia-reperfusion-induced ICAM-1 expression and its role in ischemia-reperfusion lung injury
J Appl Physiol, August 1, 2002; 93(2): 620 - 628.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Haynes Jr. and B. Obiako
Activated polymorphonuclear cells increase sickle red blood cell retention in lung: role of phospholipids
Am J Physiol Heart Circ Physiol, January 1, 2002; 282(1): H122 - H130.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. M. Dodd-O and D. B. Pearse
Effect of the NADPH oxidase inhibitor apocynin on ischemia-reperfusion lung injury
Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H303 - H312.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1998 American Thoracic Society.