help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Black, H. R.
Right arrow Articles by Noah, T. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Black, H. R.
Right arrow Articles by Noah, T. L.

Am. J. Respir. Cell Mol. Biol., Volume 19, Number 2, August, 1998 210-215

Interleukin-8 Production by Cystic Fibrosis Nasal Epithelial Cells after Tumor Necrosis Factor-alpha and Respiratory Syncytial Virus Stimulation

Hugh R. Black, James R. Yankaskas, Larry G. Johnson, and Terry L. Noah

Departments of Pediatrics and Internal Medicine, Division of Pulmonary Medicine; and Center for Enviromental Medicine and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

High levels of neutrophils and the neutrophil-attracting chemokine interleukin (IL)-8 have been observed in the airways of patients with cystic fibrosis (CF). We hypothesized that CF respiratory epithelium produces excessive amounts of IL-8 either at baseline or after stimulation. To test this hypothesis we compared immunoreactive IL-8 release by primary nasal epithelial cell (NEC) cultures established from young children with or without CF, at several time points after stimulation of cultures with tumor necrosis factor-alpha (TNF-alpha ) or infection with respiratory syncytial virus (RSV). Both stimuli induced significantly increased IL-8 release by both CF and control cultures. However, there was no difference between CF and control cells in either the magnitude or duration of the IL-8 response. The effect of transduction of CF cells with Ad5-CBCFTR, an adenovirus vector mediating expression of cystic fibrosis transmembrane regulator (CFTR), on IL-8 production was also determined. TNF-alpha stimulated IL-8 production was not different in Ad5-CBCFTR-transduced, -untransduced, or Ad5-CMVLacZ-transduced control cells. Lastly, immortalized CF tracheal epithelial cell lines, both uncorrected and retrovirally corrected with CFTR, were compared. Again, TNF-alpha -stimulated IL-8 production did not differ significantly between cell lines with and without functioning CFTR. Our data suggest that isolated CF NECs cultured under these conditions do not produce more IL-8 than do non-CF control cultures, either at baseline or after incubation with the nonspecific stimuli TNF-alpha and RSV. We conclude that the absence of functioning CFTR alone is not sufficient to cause excessive production of IL-8.




This article has been cited by other articles:


Home page
 ChestHome page
A. Elizur, C. L. Cannon, and T. W. Ferkol
Airway Inflammation in Cystic Fibrosis
Chest, February 1, 2008; 133(2): 489 - 495.
[Abstract] [Full Text] [PDF]

Home page
 Arch Otolaryngol Head Neck SurgHome page
K. Dejima, S. H. Randell, M. J. Stutts, B. A. Senior, and R. C. Boucher
Potential Role of Abnormal Ion Transport in the Pathogenesis of Chronic Sinusitis
Arch Otolaryngol Head Neck Surg, December 1, 2006; 132(12): 1352 - 1362.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
O. Zegarra-Moran, C. Folli, B. Manzari, R. Ravazzolo, L. Varesio, and L. J. V. Galietta
Double Mechanism for Apical Tryptophan Depletion in Polarized Human Bronchial Epithelium
J. Immunol., July 1, 2004; 173(1): 542 - 549.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. N. Becker, M. S. Sauer, M. S. Muhlebach, A. J. Hirsh, Q. Wu, M. W. Verghese, and S. H. Randell
Cytokine Secretion by Cystic Fibrosis Airway Epithelial Cells
Am. J. Respir. Crit. Care Med., March 1, 2004; 169(5): 645 - 653.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T. L. Noah, P. C. Murphy, J. J. Alink, M. W. Leigh, W. M. Hull, M. T. Stahlman, and J. A. Whitsett
Bronchoalveolar Lavage Fluid Surfactant Protein-A and Surfactant Protein-D Are Inversely Related to Inflammation in Early Cystic Fibrosis
Am. J. Respir. Crit. Care Med., September 15, 2003; 168(6): 685 - 691.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Li, X. D. Johnson, S. Iazvovskaia, A. Tan, A. Lin, and M. B. Hershenson
Signaling intermediates required for NF-kappa B activation and IL-8 expression in CF bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L307 - L315.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Saba, G. Soong, S. Greenberg, and A. Prince
Bacterial Stimulation of Epithelial G-CSF and GM-CSF Expression Promotes PMN Survival in CF Airways
Am. J. Respir. Cell Mol. Biol., November 1, 2002; 27(5): 561 - 567.
[Abstract] [Full Text] [PDF]

Home page
 Arch Otolaryngol Head Neck SurgHome page
S. E. Sobol, P. Christodoulopoulos, J. J. Manoukian, H.-P. Hauber, S. Frenkiel, M. Desrosiers, M. Fukakusa, M. D. Schloss, and Q. Hamid
Cytokine Profile of Chronic Sinusitis in Patients With Cystic Fibrosis
Arch Otolaryngol Head Neck Surg, November 1, 2002; 128(11): 1295 - 1298.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. Aldallal, E. E. McNaughton, L. J. Manzel, A. M. Richards, J. Zabner, T. W. Ferkol, and D. C. Look
Inflammatory Response in Airway Epithelial Cells Isolated from Patients with Cystic Fibrosis
Am. J. Respir. Crit. Care Med., November 1, 2002; 166(9): 1248 - 1256.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
M. Y. Kazachkov, P.C. Hu, J. L. Carson, P. C. Murphy, F. W. Henderson, and T. L. Noah
Release of Cytokines by Human Nasal Epithelial Cells and Peripheral Blood Mononuclear Cells Infected with Mycoplasma pneumoniae
Experimental Biology and Medicine, May 1, 2002; 227(5): 330 - 335.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. O. Daines and G. K. K. Hershey
A Novel Mechanism by Which Interferon-gamma Can Regulate Interleukin (IL)-13 Responses. EVIDENCE FOR INTRACELLULAR STORES OF IL-13 RECEPTOR alpha -2 AND THEIR RAPID MOBILIZATION BY INTERFERON-gamma
J. Biol. Chem., March 15, 2002; 277(12): 10387 - 10393.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Kube, U. Sontich, D. Fletcher, and P. B. Davis
Proinflammatory cytokine responses to P. aeruginosa infection in human airway epithelial cell lines
Am J Physiol Lung Cell Mol Physiol, March 1, 2001; 280(3): L493 - L502.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. Venkatakrishnan, A. A. Stecenko, G. King, T. R. Blackwell, K. L. Brigham, J. W. Christman, and T. S. Blackwell
Exaggerated Activation of Nuclear Factor-kappa B and Altered Ikappa B-beta Processing in Cystic Fibrosis Bronchial Epithelial Cells
Am. J. Respir. Cell Mol. Biol., September 1, 2000; 23(3): 396 - 403.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
L. Pizurki, M. A. Morris, M. Chanson, M. Solomon, A. Pavirani, I. Bouchardy, and S. Suter
Cystic Fibrosis Transmembrane Conductance Regulator Does Not Affect Neutrophil Migration across Cystic Fibrosis Airway Epithelial Monolayers
Am. J. Pathol., April 1, 2000; 156(4): 1407 - 1416.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. S. MUHLEBACH, P. W. STEWART, M. W. LEIGH, and T. L. NOAH
Quantitation of Inflammatory Responses to Bacteria in Young Cystic Fibrosis and Control Patients
Am. J. Respir. Crit. Care Med., July 1, 1999; 160(1): 186 - 191.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1998 American Thoracic Society.