help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aderibigbe, A. O.
Right arrow Articles by Auten, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aderibigbe, A. O.
Right arrow Articles by Auten, R. L., Jr.

Am. J. Respir. Cell Mol. Biol., Volume 20, Number 2, February, 1999 219-227

Brief Exposure to 95% Oxygen Alters Surfactant Protein D and mRNA in Adult Rat Alveolar and Bronchiolar Epithelium

Adegboyega O. Aderibigbe, Ronald F. Thomas, Robert R. Mercer, and Richard L. Auten Jr.

Department of Pediatrics (Neonatal Medicine), Duke University Medical Center, Durham, North Carolina; and National Institute of Occupational Safety and Health, Morgantown, West Virginia

Surfactant protein D (SP-D), which has structural homology to C-type lectin binding regions, may play a role in host defense and has no known surfactant function. Because other surfactant proteins have been shown to be increased after prolonged periods of hyperoxia, we sought to evaluate the early effects of hyperoxia (95% O2) on expression of SP-D in the adult male rat lung. Animals were exposed to air or to 12, 36, or 60 h of 95% O2. Northern blot analysis of total lung RNA revealed marked SP-D mRNA increases at 12 h 95% O2 compared with air-exposed controls, with decreasing expression to near that of air-exposed animals by 60 h. Semiquantitative in situ RNA hybridization demonstrated parallel results, with increased numbers of labeled alveolar epithelial (AE) and bronchiolar epithelial (BE) cells at 12 h and increased intensity of labeled alveolar cells, compared with air-exposed controls. After 60 h of exposure to 95% O2, mRNA label intensity in AE and BE was decreased to levels near those seen in air-exposed animals. In contrast, Western blotting showed a decline in total lung SP-D with 95% O2 exposure, beginning at 12 h and continuing at 36 and 60 h, respectively. Semiquantitative immunohistochemistry demonstrated a decline in AE labeling parallel to the total lung Western blot results, but labeled total BE cell numbers increased (P = 0.10). Hyperoxia had differential effects on SP-D abundance in AE and BE cells, and therefore may influence the availability of SP-D to bind microbial pathogens in the airways depending on cell type and location.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Jain, E. Atochina-Vasserman, H. Kadire, Y. Tomer, A. Inch, P. Scott, R. C. Savani, A. J. Gow, and M. F. Beers
SP-D-deficient mice are resistant to hyperoxia
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L861 - L871.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. Casey, J. Kaplan, E. N. Atochina-Vasserman, A. J. Gow, H. Kadire, Y. Tomer, J. H. Fisher, S. Hawgood, R. C. Savani, and M. F. Beers
Alveolar Surfactant Protein D Content Modulates Bleomycin-induced Lung Injury
Am. J. Respir. Crit. Care Med., October 1, 2005; 172(7): 869 - 877.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Dave, T. Childs, and J. A. Whitsett
Nuclear Factor of Activated T Cells Regulates Transcription of the Surfactant Protein D Gene (Sftpd) via Direct Interaction with Thyroid Transcription Factor-1 in Lung Epithelial Cells
J. Biol. Chem., August 13, 2004; 279(33): 34578 - 34588.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. N. Ahmed, H. B. Suliman, R. J. Folz, E. Nozik-Grayck, M. L. Golson, S. N. Mason, and R. L. Auten
Extracellular Superoxide Dismutase Protects Lung Development in Hyperoxia-exposed Newborn Mice
Am. J. Respir. Crit. Care Med., February 1, 2003; 167(3): 400 - 405.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. He and E. Crouch
Surfactant Protein D Gene Regulation. INTERACTIONS AMONG THE CONSERVED CCAAT/ENHANCER-BINDING PROTEIN ELEMENTS
J. Biol. Chem., May 24, 2002; 277(22): 19530 - 19537.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. T. Stahlman, M. E. Gray, W. M. Hull, and J. A. Whitsett
Immunolocalization of Surfactant Protein-D (SP-D) in Human Fetal, Newborn, and Adult Tissues
J. Histochem. Cytochem., May 1, 2002; 50(5): 651 - 660.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. L. Auten, M. H. Whorton, and S. Nicholas Mason
Blocking Neutrophil Influx Reduces DNA Damage in Hyperoxia-Exposed Newborn Rat Lung
Am. J. Respir. Cell Mol. Biol., April 1, 2002; 26(4): 391 - 397.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. L. Auten Jr., S. N. Mason, D. T. Tanaka, K. Welty-Wolf, and M. H. Whorton
Anti-neutrophil chemokine preserves alveolar development in hyperoxia-exposed newborn rats
Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L336 - L344.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
H. DENG, S. NICHOLAS MASON, and R. L. AUTEN Jr.
Lung Inflammation in Hyperoxia Can Be Prevented By Antichemokine Treatment in Newborn Rats
Am. J. Respir. Crit. Care Med., December 1, 2000; 162(6): 2316 - 2323.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1999 American Thoracic Society. 		  Red In Translatin