help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gibbs, D. F.
Right arrow Articles by Varani, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gibbs, D. F.
Right arrow Articles by Varani, J.

Am. J. Respir. Cell Mol. Biol., Volume 20, Number 6, June, 1999 1136-1144

Characterization of Matrix Metalloproteinases Produced by Rat Alveolar Macrophages

Douglas F. Gibbs, Roscoe L. Warner, Stephen J. Weiss, Kent J. Johnson, and James Varani

Departments of Pathology and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan

Evidence presented in the accompanying article (Gibbs, D. F., T. P. Shanley, R. L. Warner, H. S. Murphy, J. Varani, and K. J. Johnson. 1999. Role of matrix metalloproteinases in models of macrophage-dependent acute lung injury: evidence for alveolar macrophage as source of proteinases. Am. J. Respir. Cell Mol. Biol. 20:1145-1154) implicates alveolar macrophage matrix metalloproteinases (MMPs) in two models of acute lung inflammation in the rat. As a prerequisite to understanding which specific MMPs might be involved in the injury and how they might function, it was necessary to know the spectrum of enzymes present. To this end, alveolar macrophages were obtained from normal rat lungs by bronchoalveolar lavage, placed in culture with and without various agonists, and assessed by a variety of techniques for MMPs. The identification process involved characterization by gelatin, beta -casein, and kappa -elastin zymography, with confirmation of identity by Western blot/immunoprecipitation. Message levels of detected MMPs were assessed by Northern blot. Rat alveolar macrophages were found to produce a low constitutive level of MMP-2 (72-kD gelatinase A) that was only modestly upregulated following stimulation with phorbol myristate acetate, bacterial lipopolysaccharide, or immunoglobulin A-containing immune complexes. Although control cells were found to produce little or no MMP-9 (92-kD gelatinase B) or MMP-12 (metalloelastase), both enzymes were markedly upregulated upon stimulation. In the same stimulated macrophages there was little activity against type I collagen (associated with MMP-13 [collagenase-3] on the basis of Western blotting), no activity suggestive of stromelysin or matrilysin, and no measurable secretion of the serine proteinases, elastase and cathepsin G. These data demonstrate the ability of rat alveolar macrophages to elaborate certain MMPs under proinflammatory conditions, consistent with their possible involvement in the progression of acute inflammation.




This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
Y. Yasuda, K. Kasahara, F. Mizuno, K. Nishi, K. Mikasa, and E. Kita
Roxithromycin Favorably Modifies the Initial Phase of Resistance against Infection with Macrolide-Resistant Streptococcus pneumoniae in a Murine Pneumonia Model
Antimicrob. Agents Chemother., May 1, 2007; 51(5): 1741 - 1752.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
N. L. Webster and S. M. Crowe
Matrix metalloproteinases, their production by monocytes and macrophages and their potential role in HIV-related diseases
J. Leukoc. Biol., November 1, 2006; 80(5): 1052 - 1066.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
I K Demedts, A Morel-Montero, S Lebecque, Y Pacheco, D Cataldo, G F Joos, R A Pauwels, and G G Brusselle
Elevated MMP-12 protein levels in induced sputum from patients with COPD
Thorax, March 1, 2006; 61(3): 196 - 201.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
N. Bhagavathula, K. C. Nerusu, M. Reddy, C. N. Ellis, A. Chittiboyina, M. Avery, H. A. Pershadsingh, T. W. Kurtz, and J. Varani
BP-1107 [{2-[4-(2,4-Dioxo-thiazolidin-5-ylmethyl)-phenoxy]-ethyl}-methyl-amide]: A Novel Synthetic Thiazolidinedione That Inhibits Epidermal Hyperplasia in Psoriatic Skin-Severe-Combined Immunodeficient Mouse Transplants after Topical Application
J. Pharmacol. Exp. Ther., December 1, 2005; 315(3): 996 - 1004.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K.-H. Kim, K. Burkhart, P. Chen, C. W. Frevert, J. Randolph-Habecker, R. C. Hackman, P. D. Soloway, and D. K. Madtes
Tissue Inhibitor of Metalloproteinase-1 Deficiency Amplifies Acute Lung Injury in Bleomycin-Exposed Mice
Am. J. Respir. Cell Mol. Biol., September 1, 2005; 33(3): 271 - 279.
[Abstract] [Full Text] [PDF]

Home page
 Arch Facial Plast SurgHome page
N. Monhian, B. S. Jewett, S. R. Baker, and J. Varani
Matrix Metalloproteinase Expression in Normal Skin Associated With Basal Cell Carcinoma and in Distal Skin From the Same Patients
Arch Facial Plast Surg, July 1, 2005; 7(4): 238 - 243.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
H. Lateef, O. I. Abatan, M. N. Aslam, M. J. Stevens, and J. Varani
Topical Pretreatment of Diabetic Rats With All-trans Retinoic Acid Improves Healing of Subsequently Induced Abrasion Wounds
Diabetes, March 1, 2005; 54(3): 855 - 861.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Agorreta, J. J. Zulueta, L. M. Montuenga, and M. Garayoa
Adrenomedullin expression in a rat model of acute lung injury induced by hypoxia and LPS
Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L536 - L545.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. L. Warner, N. W. Lukacs, S. D. Shapiro, N. Bhagarvathula, K. C. Nerusu, J. Varani, and K. J. Johnson
Role of Metalloelastase in a Model of Allergic Lung Responses Induced by Cockroach Allergen
Am. J. Pathol., December 1, 2004; 165(6): 1921 - 1930.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
C. Whatling, H. Bjork, S. Gredmark, A. Hamsten, and P. Eriksson
Effect of macrophage differentiation and exposure to mildly oxidized LDL on the proteolytic repertoire of THP-1 monocytes
J. Lipid Res., September 1, 2004; 45(9): 1768 - 1776.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Churg, R. D. Wang, H. Tai, X. Wang, C. Xie, and J. L. Wright
Tumor Necrosis Factor-{alpha} Drives 70% of Cigarette Smoke-induced Emphysema in the Mouse
Am. J. Respir. Crit. Care Med., September 1, 2004; 170(5): 492 - 498.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. Lateef, M. J. Stevens, and J. Varani
All-trans-Retinoic Acid Suppresses Matrix Metalloproteinase Activity and Increases Collagen Synthesis in Diabetic Human Skin in Organ Culture
Am. J. Pathol., July 1, 2004; 165(1): 167 - 174.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
A. Bar-Or, R. K. Nuttall, M. Duddy, A. Alter, H. J. Kim, I. Ifergan, C. J. Pennington, P. Bourgoin, D. R. Edwards, and V. W. Yong
Analyses of all matrix metalloproteinase members in leukocytes emphasize monocytes as major inflammatory mediators in multiple sclerosis
Brain, December 1, 2003; 126(12): 2738 - 2749.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. L. Miller, R. M. Strieter, A. D. Gruber, S. B. Ho, and N. W. Lukacs
CXCR2 Regulates Respiratory Syncytial Virus-Induced Airway Hyperreactivity and Mucus Overproduction
J. Immunol., March 15, 2003; 170(6): 3348 - 3356.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. Varani, P. Perone, M. G. Merfert, S. E. Moon, D. Larkin, and M. J. Stevens
All-trans Retinoic Acid Improves Structure and Function of Diabetic Rat Skin in Organ Culture
Diabetes, December 1, 2002; 51(12): 3510 - 3516.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
F Ratjen, C-M Hartog, K Paul, J Wermelt, and J Braun
Matrix metalloproteases in BAL fluid of patients with cystic fibrosis and their modulation by treatment with dornase alpha
Thorax, November 1, 2002; 57(11): 930 - 934.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. E. K. Russell, A. Thorley, S. V. Culpitt, S. Dodd, L. E. Donnelly, C. Demattos, M. Fitzgerald, and P. J. Barnes
Alveolar macrophage-mediated elastolysis: roles of matrix metalloproteinases, cysteine, and serine proteases
Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L867 - L873.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. L. KANG, H. W. LEE, H. S. LEE, I. S. PACK, Y. CHONG, V. CASTRANOVA, and Y. KOH
Genistein Prevents Nuclear Factor-Kappa B Activation and Acute Lung Injury Induced by Lipopolysaccharide
Am. J. Respir. Crit. Care Med., December 15, 2001; 164(12): 2206 - 2212.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Shibata, Z. Zsengeller, K. Otake, N. Palaniyar, and B. C. Trapnell
Alveolar macrophage deficiency in osteopetrotic mice deficient in macrophage colony-stimulating factor is spontaneously corrected with age and associated with matrix metalloproteinase expression and emphysema
Blood, November 1, 2001; 98(9): 2845 - 2852.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. L. Warner, C. S. Lewis, L. Beltran, E. M. Younkin, J. Varani, and K. J. Johnson
The Role of Metalloelastase in Immune Complex-Induced Acute Lung Injury
Am. J. Pathol., June 1, 2001; 158(6): 2139 - 2144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Araya, M. Maruyama, K. Sassa, T. Fujita, R. Hayashi, S. Matsui, T. Kashii, N. Yamashita, E. Sugiyama, and M. Kobayashi
Ionizing radiation enhances matrix metalloproteinase-2 production in human lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, January 1, 2001; 280(1): L30 - L38.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W.-Y. Su, R. H. Jaskot, J. Richards, S. R. Abramson, J. F. Woessner Jr., W.-H. Yu, and K. L. Dreher
Induction of pulmonary matrilysin expression by combustion and ambient air particles
Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L152 - L160.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. D. Shapiro and R. M. Senior
Matrix Metalloproteinases . Matrix Degradation and More
Am. J. Respir. Cell Mol. Biol., June 1, 1999; 20(6): 1100 - 1102.
[Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. F. Gibbs, T. P. Shanley, R. L. Warner, H. S. Murphy, J. Varani, and K. J. Johnson
Role of Matrix Metalloproteinases in Models of Macrophage-Dependent Acute Lung Injury . Evidence for Alveolar Macrophage as Source of Proteinases
Am. J. Respir. Cell Mol. Biol., June 1, 1999; 20(6): 1145 - 1154.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1999 American Thoracic Society.