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Published ahead of print on October 23, 2009
Am. J. Respir. Cell Mol. Biol. 2009, doi:10.1165/rcmb.2009-0205OC
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Submitted on June 11, 2009
Accepted on October 23, 2009

ECG Suppresses Oxidative Stress-Induced MUC5AC Overexpression by Interaction with EGFR

Hyun Jik Kim1, Ji-Hwan Ryu2, Chang-Hoon Kim3, Jun Wan Lim4, Uk Yeol Moon5, Gyu Ho Lee1, Jeung-Gweon Lee4, Seung Joon Baek6, and Joo-Heon Yoon7*

1 Otolaryngology, Head and Neck Surgery, Chung-Ang University Collegel of Medicine, Seoul, Korea, Republic of, 2 Research Center for Human Natural Defense System, Seoul, Korea, Republic of, 3 Otorhinolaryngology, Yonsei University, College of Medicine, Seoul, Korea, Republic of; The Airway Mucus Institute, Yonsei University, College of Medicine, Seoul, Korea, Republic of, 4 Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea, Republic of, 5 The Airway Mucus Institute, Yonsei University, College of Medicine, Seoul, Korea, Republic of, 6 Department of Pathobiology, University of Tennessee, College of Veterinary Medicine, Tennessee, United States, 7 Otorhinolaryngology, Yonsei University, College of Medicine, Seoul, Korea, Republic of; The Airway Mucus Institute, Yonsei University, College of Medicine , Seoul, Korea, Republic of; The Brain Korea 21 Project for Medical Science, Yonsei University, College of Medicine , Seoul, Korea, Republic of; Research Center for Human Natural Defense System, Yonsei University, College of Medicine, Seoul, Korea, Republic of

* To whom correspondence should be addressed. E-mail: jhyoon{at}yuhs.ac.

The goal of this study was to investigate the effect of epicatechin gallate (ECG), a component of green tea polyphenols, on the signal pathway for oxidative stress-induced intracellular reactive oxygen species (ROS) generation and MUC5AC overexpression in normal human nasal epithelial (NHNE) cells. Passage-2 NHNE cells were used. ECG was administered before stimulation with exogenous H2O2. MUC5AC gene and protein level were measured with real-time PCR and dot blot analysis. Western blot analysis and immunocytofluorescence study were carried out for detecting the activity of epidermal growth factor receptor (EGFR). Exogenous H2O2 increases intracellular ROS generation, leading to the overexpression of MUC5AC. The phosphorylation and internalization of EGFR were associated with this ROS generation. ECG decreased the phosphorylation and internalization of EGFR at the cell surface of NHNE cells, resulting in the attenuation of exogenous H2O2-induced intracellular ROS generation and MUC5AC overexpression. ECG may be a therapeutic material against oxidative stress-induced ROS generation and mucus hypersecretion in airways.
Key words: Epicatechin Gallate • Epidermal growth factor receptor • Mucus hypersecretion • Oxidative stress






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