help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tokieda, K.
Right arrow Articles by Whitsett, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tokieda, K.
Right arrow Articles by Whitsett, J. A.

Am. J. Respir. Cell Mol. Biol., Volume 21, Number 4, October, 1999 463-472

Surfactant Protein-B-Deficient Mice Are Susceptible to Hyperoxic Lung Injury

Keisuke Tokieda, Harriet S. Iwamoto, Cindy Bachurski, Susan E. Wert, William M. Hull, Kazushige Ikeda, and Jeffrey A. Whitsett

Division of Neonatology and Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio

Surfactant protein-B (SP-B) is a small, hydrophobic peptide that plays a critical role in pulmonary function and surfactant homeostasis. To determine whether SP-B protects mice from oxygen-induced injury, heterozygous SP-B+/- gene-targeted mice and wild-type SP-B+/+ littermates were exposed to hyperoxia (95% oxygen for 3 d) or room air. Although specific lung compliance in room air in SP-B+/- mice was slightly reduced as compared with that in SP-B+/+ mice, it was reduced more markedly during hyperoxia (46% versus 25% decrease, respectively). The larger decrease in lung compliance in SP-B+/- mice was associated with increased severity of pulmonary edema, hemorrhage and inflammation, lung permeability and protein leakage into the alveolar space. Hyperoxia increased SP-B messenger RNA (mRNA) and total protein concentrations by 2-fold in SP-B+/+ and SP-B+/- mice, but decreased the abundance of SP-B protein in lavage fluid relative to total protein only in SP-B+/- mice. Hyperoxia increased SP-B expression, but apparently not enough to maintain SP-B function and lung compliance in the presence of increased protein leakage in SP-B+/- mice. Increased alveolar-capillary leakage and relative deficiency of SP-B may therefore contribute to oxygen-induced pulmonary dysfunction in SP-B+/- mice. These data support the concept that SP-B plays an important protective role in the lung.




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Matsuzaki, V. Besnard, J. C. Clark, Y. Xu, S. E. Wert, M. Ikegami, and J. A. Whitsett
STAT3 Regulates ABCA3 Expression and Influences Lamellar Body Formation in Alveolar Type II Cells
Am. J. Respir. Cell Mol. Biol., May 1, 2008; 38(5): 551 - 558.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Jain, E. Atochina-Vasserman, H. Kadire, Y. Tomer, A. Inch, P. Scott, R. C. Savani, A. J. Gow, and M. F. Beers
SP-D-deficient mice are resistant to hyperoxia
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L861 - L871.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Akei, J. A. Whitsett, M. Buroker, T. Ninomiya, H. Tatsumi, T. E. Weaver, and M. Ikegami
Surface tension influences cell shape and phagocytosis in alveolar macrophages
Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L572 - L579.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. H. Thomas, P. Meyn, and N. Suttorp
Single nucleotide polymorphism in 5'-flanking region reduces transcription of surfactant protein B gene in H441 cells
Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L386 - L390.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, J. A. Whitsett, P. C. Martis, and T. E. Weaver
Reversibility of lung inflammation caused by SP-B deficiency
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L962 - L970.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. L. Martin, L. A. McCaig, B. Z. Moyer, M. C. Pape, K. J. Leco, J. F. Lewis, and R. A. W. Veldhuizen
Differential response of TIMP-3 null mice to the lung insults of sepsis, mechanical ventilation, and hyperoxia
Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L244 - L251.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, T. D. Le Cras, W. D. Hardie, M. T. Stahlman, J. A. Whitsett, and T. R. Korfhagen
TGF-{alpha} perturbs surfactant homeostasis in vivo
Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L34 - L43.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. L. Nesslein, K. R. Melton, M. Ikegami, C.-L. Na, S. E. Wert, W. R. Rice, J. A. Whitsett, and T. E. Weaver
Partial SP-B deficiency perturbs lung function and causes air space abnormalities
Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1154 - L1161.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. A. Whitsett, C. J. Bachurski, K. C. Barnes, P. A. Bunn Jr., L. M. Case, D. N. Cook, D. Crooks, M. W. Duncan, L. Dwyer-Nield, R. C. Elston, et al.
Functional Genomics of Lung Disease
Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2/S1): S1 - S81.
[Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Rova, R. Haataja, R. Marttila, V. Ollikainen, O. Tammela, and M. Hallman
Data mining and multiparameter analysis of lung surfactant protein genes in bronchopulmonary dysplasia
Hum. Mol. Genet., June 1, 2004; 13(11): 1095 - 1104.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Hokuto, A.-K. T. Perl, and J. A. Whitsett
FGF signaling is required for pulmonary homeostasis following hyperoxia
Am J Physiol Lung Cell Mol Physiol, March 1, 2004; 286(3): L580 - L587.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Pagano, Y. Donati, I. Metrailler, and C. Barazzone Argiroffo
Mitochondrial cytochrome c release is a key event in hyperoxia-induced lung injury: protection by cyclosporin A
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L275 - L283.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. N. Gong, Z. Wei, L.-L. Xu, D. P. Miller, B. T. Thompson, and D. C. Christiani
Polymorphism in the Surfactant Protein-B Gene, Gender, and the Risk of Direct Pulmonary Injury and ARDS
Chest, January 1, 2004; 125(1): 203 - 211.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. R. Melton, L. L. Nesslein, M. Ikegami, J. W. Tichelaar, J. C. Clark, J. A. Whitsett, and T. E. Weaver
SP-B deficiency causes respiratory failure in adult mice
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L543 - L549.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. Epaud, M. Ikegami, J. A. Whitsett, A. H. Jobe, T. E. Weaver, and H. T. Akinbi
Surfactant Protein B Inhibits Endotoxin-Induced Lung Inflammation
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 373 - 378.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
S. Dauger, L. Ferkdadji, G. Saumon, G. Vardon, M. Peuchmaur, C. Gaultier, and J. Gallego
Neonatal Exposure to 65% Oxygen Durably Impairs Lung Architecture and Breathing Pattern in Adult Mice
Chest, February 1, 2003; 123(2): 530 - 538.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
J. A. Whitsett and T. E. Weaver
Hydrophobic Surfactant Proteins in Lung Function and Disease
N. Engl. J. Med., December 26, 2002; 347(26): 2141 - 2148.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. F. Seymour and J. J. Presneill
Pulmonary Alveolar Proteinosis: Progress in the First 44 Years
Am. J. Respir. Crit. Care Med., July 15, 2002; 166(2): 215 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
W. D. Hardie, D. R. Prows, A. Piljan-Gentle, M. R. Dunlavy, S. C. Wesselkamper, G. D. Leikauf, and T. R. Korfhagen
Dose-Related Protection from Nickel-Induced Lung Injury in Transgenic Mice Expressing Human Transforming Growth Factor-alpha
Am. J. Respir. Cell Mol. Biol., April 1, 2002; 26(4): 430 - 437.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. M. Krane, C. N. Fortner, A. R. Hand, D. W. McGraw, J. N. Lorenz, S. E. Wert, J. E. Towne, R. J. Paul, J. A. Whitsett, and A. G. Menon
Aquaporin 5-deficient mouse lungs are hyperresponsive to cholinergic stimulation
PNAS, November 9, 2001; (2001) 231273398.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
E. P. Ingenito, R. Mora, M. Cullivan, Y. Marzan, K. Haley, L. Mark, and L. A. Sonna
Decreased Surfactant Protein-B Expression and Surfactant Dysfunction in a Murine Model of Acute Lung Injury
Am. J. Respir. Cell Mol. Biol., July 1, 2001; 25(1): 35 - 44.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Ikegami, A. H. Jobe, J. Whitsett, and T. Korfhagen
Tolerance of SP-A-deficient mice to hyperoxia or exercise
J Appl Physiol, August 1, 2000; 89(2): 644 - 648.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
L. M. NOGEE, S. E. WERT, S. A. PROFFIT, W. M. HULL, and J. A. WHITSETT
Allelic Heterogeneity in Hereditary Surfactant Protein B (SP-B) Deficiency
Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): 973 - 981.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. E. GREENE, J. R. WRIGHT, K. P. STEINBERG, J. T. RUZINSKI, E. CALDWELL, W. B. WONG, W. HULL, J. A. WHITSETT, T. AKINO, Y. KUROKI, et al.
Serial Changes in Surfactant-associated Proteins in Lung and Serum before and after Onset of ARDS
Am. J. Respir. Crit. Care Med., December 1, 1999; 160(6): 1843 - 1850.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Hickman-Davis and S. Matalon
Surfactant Protein B Deficiency Worsens Hyperoxic Injury to the Alveolar Epithelium
Am. J. Respir. Cell Mol. Biol., October 1, 1999; 21(4): 449 - 450.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. M. Krane, C. N. Fortner, A. R. Hand, D. W. McGraw, J. N. Lorenz, S. E. Wert, J. E. Towne, R. J. Paul, J. A. Whitsett, and A. G. Menon
Aquaporin 5-deficient mouse lungs are hyperresponsive to cholinergic stimulation
PNAS, November 20, 2001; 98(24): 14114 - 14119.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. V. Kalinichenko, Y. Zhou, B. Shin, D. B. Stolz, S. C. Watkins, J. A. Whitsett, and R. H. Costa
Wild-type levels of the mouse Forkhead Box f1 gene are essential for lung repair
Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1253 - L1265.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1999 American Thoracic Society.