help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hamelmann, E.
Right arrow Articles by Gelfand, E. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamelmann, E.
Right arrow Articles by Gelfand, E. W.

Am. J. Respir. Cell Mol. Biol., Volume 21, Number 4, October, 1999 480-489

Development of Eosinophilic Airway Inflammation and Airway Hyperresponsiveness Requires Interleukin-5 but Not Immunoglobulin E or B Lymphocytes

Eckard Hamelmann, Katsuyuki Takeda, Jurgen Schwarze, Anthony T. Vella, Charles G. Irvin, and Erwin W. Gelfand

Division of Basic Sciences and Departments of Pediatrics and Medicine, National Jewish Medical and Research Center; and The Howard Hughes Medical Institute, Denver, Colorado

We previously defined a role for B cells and allergen-specific immunoglobulins in the development of allergic sensitization, airway inflammation, and airway hyperresponsiveness (AHR), using a 10-d protocol in which allergen exposure occurred exclusively via the airways, without adjuvant. In the present protocol, normal and B-cell-deficient (µMt-/-) mice were sensitized intraperitoneally to ovalbumin (OVA) and challenged with OVA via the airways in order to examine the requirements for AHR with this protocol. T-cell activation (antigen-specific proliferative responses and Th2-type cytokine production) and eosinophil infiltration in the peribronchial regions of the airways, with signs of eosinophil activation and degranulation, occurred in both experimental groups. In contrast to the 10-d protocol, increased in vivo airway responsiveness to methacholine and in vitro tracheal smooth-muscle responses to electrical field stimulation were observed in both normal and B-cell-deficient mice, and these responses were inhibited by anti-interleukin (IL)-5 administration before airway challenge. These data show that IL-5, but not B cells or allergen-specific IgE, are required for eosinophil airway infiltration and the development of AHR following allergen/alum sensitization and repeated airway challenge with allergen. These results emphasize that the use of different sensitization and challenge protocols can influence the requirements for development of AHR.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
C. Kruschinski, T. Skripuletz, S. Bedoui, K. Raber, R. H. Straub, T. Hoffmann, K. Grote, R. Jacobs, M. Stephan, R. Pabst, et al.
Postnatal Life Events Affect the Severity of Asthmatic Airway Inflammation in the Adult Rat
J. Immunol., March 15, 2008; 180(6): 3919 - 3925.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. C. Pitchford, S. Momi, S. Baglioni, L. Casali, S. Giannini, R. Rossi, C. P. Page, and P. Gresele
Allergen Induces the Migration of Platelets to Lung Tissue in Allergic Asthma
Am. J. Respir. Crit. Care Med., March 15, 2008; 177(6): 604 - 612.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
K. C. Beier, T. Kallinich, and E. Hamelmann
T-cell co-stimulatory molecules: novel targets for the treatment of allergic airway disease
Eur. Respir. J., August 1, 2007; 30(2): 383 - 390.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. P. Matson, L. Zhu, E. G. Lingenheld, C. M. Schramm, R. B. Clark, D. M. Selander, R. S. Thrall, E. Breen, and L. Puddington
Maternal Transmission of Resistance to Development of Allergic Airway Disease
J. Immunol., July 15, 2007; 179(2): 1282 - 1291.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
T. Kallinich, K. C. Beier, U. Wahn, P. Stock, and E. Hamelmann
T-cell co-stimulatory molecules: their role in allergic immune reactions
Eur. Respir. J., June 1, 2007; 29(6): 1246 - 1255.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Skripuletz, A. Schmiedl, J. Schade, S. Bedoui, T. Glaab, R. Pabst, S. von Horsten, and M. Stephan
Dose-dependent recruitment of CD25+ and CD26+ T cells in a novel F344 rat model of asthma
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1564 - L1571.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
M. Witzenrath, B. Ahrens, S. M. Kube, A. C. Hocke, S. Rosseau, E. Hamelmann, N. Suttorp, and H. Schutte
Allergic lung inflammation induces pulmonary vascular hyperresponsiveness
Eur. Respir. J., August 1, 2006; 28(2): 370 - 377.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Taube, J. M. Thurman, K. Takeda, A. Joetham, N. Miyahara, M. C. Carroll, A. Dakhama, P. C. Giclas, V. M. Holers, and E. W. Gelfand
Factor B of the alternative complement pathway regulates development of airway hyperresponsiveness and inflammation
PNAS, May 23, 2006; 103(21): 8084 - 8089.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Matsubara, T. Koya, K. Takeda, A. Joetham, N. Miyahara, P. Pine, E. S. Masuda, C. H. Swasey, and E. W. Gelfand
Syk Activation in Dendritic Cells Is Essential for Airway Hyperresponsiveness and Inflammation
Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 426 - 433.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Thurman and V. M. Holers
The Central Role of the Alternative Complement Pathway in Human Disease
J. Immunol., February 1, 2006; 176(3): 1305 - 1310.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. Matsubara, G. Li, K. Takeda, J. E. Loader, P. Pine, E. S. Masuda, N. Miyahara, S. Miyahara, J. J. Lucas, A. Dakhama, et al.
Inhibition of Spleen Tyrosine Kinase Prevents Mast Cell Activation and Airway Hyperresponsiveness
Am. J. Respir. Crit. Care Med., January 1, 2006; 173(1): 56 - 63.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Terawaki, T. Yokomizo, T. Nagase, A. Toda, M. Taniguchi, K. Hashizume, T. Yagi, and T. Shimizu
Absence of Leukotriene B4 Receptor 1 Confers Resistance to Airway Hyperresponsiveness and Th2-Type Immune Responses
J. Immunol., October 1, 2005; 175(7): 4217 - 4225.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. P. R. Sutherland, L. G. Ng, C. A. Fletcher, B. Shum, R. A. Newton, S. T. Grey, M. S. Rolph, F. Mackay, and C. R. Mackay
BAFF Augments Certain Th1-Associated Inflammatory Responses
J. Immunol., May 1, 2005; 174(9): 5537 - 5544.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. D. Finkelman, M. Yang, C. Perkins, K. Schleifer, A. Sproles, J. Santeliz, J. A. Bernstein, M. E. Rothenberg, S. C. Morris, and M. Wills-Karp
Suppressive Effect of IL-4 on IL-13-Induced Genes in Mouse Lung
J. Immunol., April 15, 2005; 174(8): 4630 - 4638.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
D. P. Ennis, J. P. Cassidy, and B. P. Mahon
Acellular Pertussis Vaccine Protects against Exacerbation of Allergic Asthma Due to Bordetella pertussis in a Murine Model
Clin. Vaccine Immunol., March 1, 2005; 12(3): 409 - 417.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Wagers, L. K. A. Lundblad, M. Ekman, C. G. Irvin, and J. H. T. Bates
The allergic mouse model of asthma: normal smooth muscle in an abnormal lung?
J Appl Physiol, June 1, 2004; 96(6): 2019 - 2027.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
K. Clark, L. Simson, N. Newcombe, A. M. L. Koskinen, J. Mattes, N. A. Lee, J. J. Lee, L. A. Dent, K. I. Matthaei, and P. S. Foster
Eosinophil degranulation in the allergic lung of mice primarily occurs in the airway lumen
J. Leukoc. Biol., June 1, 2004; 75(6): 1001 - 1009.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Chen, K. A. Grabowski, J.-p. Xin, J. Coleman, Z. Huang, B. Espiritu, S. Alkan, H. B. Xie, Y. Zhu, F. A. White, et al.
IL-4 Induces Differentiation and Expansion of Th2 Cytokine-Producing Eosinophils
J. Immunol., February 15, 2004; 172(4): 2059 - 2066.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. Taube, Y.-H. Rha, K. Takeda, J.-W. Park, A. Joetham, A. Balhorn, A. Dakhama, P. C. Giclas, V. M. Holers, and E. W. Gelfand
Inhibition of Complement Activation Decreases Airway Inflammation and Hyperresponsiveness
Am. J. Respir. Crit. Care Med., December 1, 2003; 168(11): 1333 - 1341.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. B. Hogan, D. N. Weissman, A. F. Hubbs, L. F. Gibson, D. Piktel, and K. S. Landreth
Regulation of Eosinophilopoiesis in a Murine Model of Asthma
J. Immunol., September 1, 2003; 171(5): 2644 - 2651.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. P. Justice, M. T. Borchers, J. R. Crosby, E. M. Hines, H. H. Shen, S. I. Ochkur, M. P. McGarry, N. A. Lee, and J. J. Lee
Ablation of eosinophils leads to a reduction of allergen-induced pulmonary pathology
Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L169 - L178.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
J Douwes, P Gibson, J Pekkanen, and N Pearce
Non-eosinophilic asthma: importance and possible mechanisms
Thorax, July 1, 2002; 57(7): 643 - 648.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D.-w. Jeong, M.-H. Yoo, T. S. Kim, J.-H. Kim, and I. Y. Kim
Protection of Mice from Allergen-induced Asthma by Selenite. PREVENTION OF EOSINOPHIL INFILTRATION BY INHIBITION OF NF-kappa B ACTIVATION
J. Biol. Chem., May 10, 2002; 277(20): 17871 - 17876.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. R. Crosby, G. Cieslewicz, M. Borchers, E. Hines, P. Carrigan, J. J. Lee, and N. A. Lee
Early Phase Bronchoconstriction in the Mouse Requires Allergen-Specific IgG
J. Immunol., April 15, 2002; 168(8): 4050 - 4054.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
I. Topilski, A. Harmelin, R. A. Flavell, Y. Levo, and I. Shachar
Preferential Th1 Immune Response in Invariant Chain-Deficient Mice
J. Immunol., February 15, 2002; 168(4): 1610 - 1617.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. T. Borchers, J. Crosby, P. Justice, S. Farmer, E. Hines, J. J. Lee, and N. A. Lee
Intrinsic AHR in IL-5 transgenic mice is dependent on CD4+ cells and CD49d-mediated signaling
Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L653 - L659.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Takeda, A. Haczku, J. J. Lee, C. G. Irvin, and E. W. Gelfand
Strain dependence of airway hyperresponsiveness reflects differences in eosinophil localization in the lung
Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L394 - L402.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. M. Fleming, H. He, A. Ciota, D. Perkins, and P. W. Finn
Administration of Pentoxifylline During Allergen Sensitization Dissociates Pulmonary Allergic Inflammation from Airway Hyperresponsiveness
J. Immunol., August 1, 2001; 167(3): 1703 - 1711.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Malm-Erjefalt, C. G. A. Persson, and J. S. Erjefalt
Degranulation Status of Airway Tissue Eosinophils in Mouse Models of Allergic Airway Inflammation
Am. J. Respir. Cell Mol. Biol., March 1, 2001; 24(3): 352 - 359.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. Bautsch, H.-G. Hoymann, Q. Zhang, I. Meier-Wiedenbach, U. Raschke, R. S. Ames, B. Sohns, N. Flemme, A. Meyer zu Vilsendorf, M. Grove, et al.
Cutting Edge: Guinea Pigs with a Natural C3a-Receptor Defect Exhibit Decreased Bronchoconstriction in Allergic Airway Disease: Evidence for an Involvement of the C3a Anaphylatoxin in the Pathogenesis of Asthma
J. Immunol., November 15, 2000; 165(10): 5401 - 5405.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
E. Hamelmann, K. Takeda, A. Haczku, G. Cieslewicz, L. Shultz, Q. Hamid, Z. Xing, J. Gauldie, and E. W. Gelfand
Interleukin (IL)-5 but Not Immunoglobulin E Reconstitutes Airway Inflammation and Airway Hyperresponsiveness in IL-4-Deficient Mice
Am. J. Respir. Cell Mol. Biol., September 1, 2000; 23(3): 327 - 334.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. S. Foster
Allergic Networks Regulating Eosinophilia
Am. J. Respir. Cell Mol. Biol., October 1, 1999; 21(4): 451 - 454.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 1999 American Thoracic Society.