Am. J. Respir. Cell Mol. Biol., Volume 22, Number 3, March, 2000 280-288

Degree of Lung Maturity Determines the Direction of the Interleukin-1- Induced Effect on the Expression of Surfactant Proteins

Virpi Glumoff, Outi Väyrynen, Tiina Kangas, and Mikko Hallman

Department of Pediatrics and Biocenter Oulu, University of Oulu, Oulu, Finland

Intra-amniotic interleukin (IL)-1 increases surfactant components in immature fetal lung, whereas high IL-1 after birth is associated with surfactant dysfunction. Our aim was to investigate whether the fetal age influences the responsiveness of surfactant proteins (SPs) to IL-1. Rabbit lung explants from fetuses at 19, 22, 27, and 30 d of gestation and 1-d-old newborns were cultured in serum-free medium in the presence of recombinant human (rh) IL-1 or vehicle. The influence of IL-1 on SP-A, -B, and -C messenger RNA (mRNA) content was dependent on the conceptional age. In very immature lung on Day 19, rhIL-1 (570 ng/ml for 20 h) increased SP-A, -B, and -C mRNA by 860 ± 15%, 314 ± 108%, and 64 ± 17%, respectively. The increase in SP-A mRNA was evident within 4 to 6 h. IL-1 increased the SP-A concentration in alveolar epithelial cells and in the culture medium within 20 h. In contrast, at 27 to 30 d of gestation and in newborns, IL-1 decreased SP-C, -B, and -A mRNA by means of 64 to 67%, 48 to 59%, and 12 to 15%, respectively. SP-B protein decreased by 45 to 60%. The decrease in mRNA became evident within 8 to 12 h and was dependent on IL-1 concentration. On Day 27, IL-1 accelerated the degradation of SP-B mRNA in the presence of actinomycin D. IL-1 did not increase the degradation rate of SP-A mRNA unless both actinomycin D and cycloheximide were added to the explants. The present findings may explain some of the contrasting associations between inflammatory cytokines and lung diseases during the perinatal period. The determinants of the direction of the IL-1 effect on the expression of SPs remain to be identified.

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