help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ahmed, S. I.
Right arrow Articles by Woll, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ahmed, S. I.
Right arrow Articles by Woll, P. J.

Am. J. Respir. Cell Mol. Biol., Volume 22, Number 4, April, 2000 422-431

Studies on the Expression of Endothelin, Its Receptor Subtypes, and Converting Enzymes in Lung Cancer and in Human Bronchial Epithelium

Samreen I. Ahmed, James Thompson, Judy M. Coulson, and Penella J. Woll

Cancer Research Campaign, Academic Department of Clinical Oncology, University of Nottingham, Nottingham City Hospital, Nottingham, United Kingdom

Lung cancer, particularly small cell lung cancer (SCLC), is characterized by production of numerous peptides and their resulting clinical syndromes. Such peptides can act as autocrine growth factors for these tumors. In this study, we investigated the role of endothelin (ET)-1 in lung cancer. Using reverse transcription/polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay, and immunocytochemistry, we screened a panel of lung cancer cell lines for ET-1, its receptors, and endothelin converting enzyme-1 (ECE-1), which generates the active form of ET-1. ET-1 messenger RNA was expressed in five of seven SCLC, four of four non-small cell lung cancer (NSCLC), and human bronchial epithelial (HBE) cells. The intracellular isoform of ECE-1, important in processing ET-1 if an autocrine growth loop is to function, was downregulated in the lung cancer cell lines as compared with expression of the extracellular isoform. Endothelin A receptor (ETAR), which mediates the mitogenic effects of ET-1 in prostate and ovarian cancer, was upregulated in HBE cells compared with expression in three of seven SCLC and two of four NSCLC cell lines. Endothelin B receptor (ETBR) was more widespread, being expressed in seven of seven SCLC, four of four NSCLC, and the HBE cells. We used flow cytometry to measure mobilization of intracellular calcium as a functional assay for the ETAR. These data concurred with the RT-PCR results, indicating that the ETAR was downregulated or was involved in an alternative signal transduction pathway in lung cancer, and no evidence of functional receptor mediating an autocrine growth loop was found. From our study, the data do not support the putative functional autocrine growth role of ET-1 in lung cancer. We propose instead that ET-1 may act as a paracrine growth factor for surrounding epithelial and endothelial cells via alternative pathways, promoting angiogenesis and stromal growth.




This article has been cited by other articles:


Home page
 ChestHome page
P. S. Hodkinson, A. MacKinnon, and T. Sethi
Targeting Growth Factors in Lung Cancer
Chest, May 1, 2008; 133(5): 1209 - 1216.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Surg. Oncol.Home page
H. Namazi
A Novel Use of Botulinum Toxin to Ameliorate Bone Cancer Pain
Ann. Surg. Oncol., April 1, 2008; 15(4): 1259 - 1260.
[Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
G. M Tse, B. Chaiwun, K.-M. Lau, R. Scolyer, C S. Lee, R. Z Karim, T. C Putti, B. K Law, P. C Lui, and P. H. Tan
Endothelin-1 expression correlates with atypical histological features in mammary phyllodes tumours
J. Clin. Pathol., September 1, 2007; 60(9): 1051 - 1056.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. Jain, P. W. Shaul, Z. Borok, and B. C. Willis
Endothelin-1 Induces Alveolar Epithelial-Mesenchymal Transition through Endothelin Type A Receptor-Mediated Production of TGF-beta1
Am. J. Respir. Cell Mol. Biol., July 1, 2007; 37(1): 38 - 47.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
L. Rosano, V. Di Castro, F. Spinella, M. R. Nicotra, P. G. Natali, and A. Bagnato
ZD4054, a specific antagonist of the endothelin A receptor, inhibits tumor growth and enhances paclitaxel activity in human ovarian carcinoma in vitro and in vivo
Mol. Cancer Ther., July 1, 2007; 6(7): 2003 - 2011.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
I. Fishel, A. Kaufman, and E. Ruppin
Meta-analysis of gene expression data: a predictor-based approach
Bioinformatics, July 1, 2007; 23(13): 1599 - 1606.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
M J Grimshaw
Endothelins and hypoxia-inducible factor in cancer
Endocr. Relat. Cancer, June 1, 2007; 14(2): 233 - 244.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
R. Benoliel, J. Epstein, E. Eliav, R. Jurevic, and S. Elad
Orofacial Pain in Cancer: Part I--Mechanisms
J. Dent. Res., June 1, 2007; 86(6): 491 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
D. Ribatti, M. T. Conconi, and G. G. Nussdorfer
Nonclassic Endogenous Novel Regulators of Angiogenesis
Pharmacol. Rev., June 1, 2007; 59(2): 185 - 205.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
B. Houweling, D. Merkus, O. Sorop, F. Boomsma, and D. J. Duncker
Role of endothelin receptor activation in secondary pulmonary hypertension in awake swine after myocardial infarction
J. Physiol., July 15, 2006; 574(2): 615 - 626.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Milan, C. Charalambous, R. Elhag, T. C. Chen, W. Li, S. Guan, F. M. Hofman, and R. Zidovetzki
Multiple signaling pathways are involved in endothelin-1-induced brain endothelial cell migration
Am J Physiol Cell Physiol, July 1, 2006; 291(1): C155 - C164.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
L. A. Dawson, N. J. Maitland, P. Berry, A. J. Turner, and B. A. Usmani
Expression and localization of endothelin-converting enzyme-1 in human prostate cancer.
Experimental Biology and Medicine, June 1, 2006; 231(6): 1106 - 1110.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. C. Reubi
Peptide Receptors as Molecular Targets for Cancer Diagnosis and Therapy
Endocr. Rev., August 1, 2003; 24(4): 389 - 427.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Blouquit, A. Sari, A. Lombet, M. D'herbomez, E. Naline, R. Matran, and T. Chinet
Effects of Endothelin-1 on Epithelial Ion Transport in Human Airways
Am. J. Respir. Cell Mol. Biol., August 1, 2003; 29(2): 245 - 251.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
M. J. Grimshaw, S. Naylor, and F. R. Balkwill
Endothelin-2 Is a Hypoxia-induced Autocrine Survival Factor for Breast Tumor Cells
Mol. Cancer Ther., December 1, 2002; 1(14): 1273 - 1281.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. Favier, P.-F. Plouin, P. Corvol, and J.-M. Gasc
Angiogenesis and Vascular Architecture in Pheochromocytomas : Distinctive Traits in Malignant Tumors
Am. J. Pathol., October 1, 2002; 161(4): 1235 - 1246.
[Abstract] [Full Text] [PDF]

Home page
 JNMHome page
S. Aleksic, Z. Szabo, U. Scheffel, H. T. Ravert, W. B. Mathews, L. Kerenyi, P. A. Rauseo, R. E. Gibson, H. D. Burns, and R. F. Dannals
In Vivo Labeling of Endothelin Receptors with [11C]L-753,037: Studies in Mice and a Dog
J. Nucl. Med., August 1, 2001; 42(8): 1274 - 1280.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society.