help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brouns, I.
Right arrow Articles by Timmermans, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brouns, I.
Right arrow Articles by Timmermans, J.-P.

Am. J. Respir. Cell Mol. Biol., Volume 23, Number 1, July, 2000 52-61

Intraepithelial Vagal Sensory Nerve Terminals in Rat Pulmonary Neuroepithelial Bodies Express P2X3 Receptors

Inge Brouns, Dirk Adriaensen, Geoff Burnstock, and Jean-Pierre Timmermans

Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium; and Autonomic Neuroscience Institute, Royal Free Hospital School of Medicine, London, United Kingdom

The neurotransmitters/modulators involved in the interaction between pulmonary neuroepithelial bodies (NEBs) and the vagal sensory component of their innervation have not yet been elucidated. Because P2X3 purinoreceptors are known to be strongly expressed in peripheral sensory neurons, the aim of the present study was to examine the localization of nerve endings expressing P2X3 purinoreceptors in the rat lung in general and those contacting pulmonary NEBs in particular. Most striking were intraepithelial arborizations of P2X3 purinoceptor-immunoreactive (IR) nerve terminals, which in all cases appeared to ramify between calcitonin gene-related peptide (CGRP)- or calbindin D28k (CB)-labeled NEB cells. However, not all NEBs received nerve endings expressing P2X3 receptors. Using CGRP and CB staining as markers for two different sensory components of the innervation of NEBs, it was revealed that P2X3 receptor and CB immunoreactivity were colocalized, whereas CGRP-IR fibers clearly formed a different population. The disappearance of characteristic P2X3 receptor-positive nerve fibers in contact with NEBs after infranodosal vagal crush and colocalization of tracer and P2X3 receptor immunoreactivity in vagal nodose neuronal cell bodies in retrograde tracing experiments further supports our hypothesis that the P2X3 receptor-IR nerve fibers contacting NEBs have their origin in the vagal sensory nodose ganglia. Combination of quinacrine accumulation in NEBs, suggestive of the presence of high concentrations of adenosine triphosphate (ATP) in their secretory vesicles, and P2X3 receptor staining showed that the branching intraepithelial P2X3 receptor-IR nerve terminals in rat lungs were exclusively associated with quinacrine-stained NEBs. We conclude that ATP might act as a neurotransmitter/neuromodulator in the vagal sensory innervation of NEBs via a P2X3 receptor-mediated pathway. Further studies are necessary to determine whether the P2X3 receptor-expressing neurons, specifically innervating NEBs in the rat lung, belong to a population of P2X3 receptor-IR nociceptive vagal nodose neurons.




This article has been cited by other articles:


Home page
 FASEB J.Home page
I. De Proost, I. Pintelon, W. J. Wilkinson, S. Goethals, I. Brouns, L. Van Nassauw, D. Riccardi, J.-P. Timmermans, P. J. Kemp, and D. Adriaensen
Purinergic signaling in the pulmonary neuroepithelial body microenvironment unraveled by live cell imaging
FASEB J, April 1, 2009; 23(4): 1153 - 1160.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
I. Pintelon, I. Brouns, I. De Proost, F. Van Meir, J.-P. Timmermans, and D. Adriaensen
Sensory Receptors in the Visceral Pleura: Neurochemical Coding and Live Staining in Whole Mounts
Am. J. Respir. Cell Mol. Biol., May 1, 2007; 36(5): 541 - 551.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Burnstock
Physiology and Pathophysiology of Purinergic Neurotransmission
Physiol Rev, April 1, 2007; 87(2): 659 - 797.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. Adriaensen, I. Brouns, I. Pintelon, I. De Proost, and J.-P. Timmermans
Evidence for a role of neuroepithelial bodies as complex airway sensors: comparison with smooth muscle-associated airway receptors
J Appl Physiol, September 1, 2006; 101(3): 960 - 970.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
G. Burnstock
Pathophysiology and therapeutic potential of purinergic signaling.
Pharmacol. Rev., March 1, 2006; 58(1): 58 - 86.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Pan, I. Copland, M. Post, H. Yeger, and E. Cutz
Mechanical stretch-induced serotonin release from pulmonary neuroendocrine cells: implications for lung development
Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L185 - L193.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. Dang, K. Bielfeldt, K. Lamb, and G. F. Gebhart
Gastric Ulcers Evoke Hyperexcitability and Enhance P2X Receptor Function in Rat Gastric Sensory Neurons
J Neurophysiol, June 1, 2005; 93(6): 3112 - 3119.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Van Genechten, I. Brouns, G. Burnstock, J.-P. Timmermans, and D. Adriaensen
Quantification of Neuroepithelial Bodies and Their Innervation in Fawn-Hooded and Wistar Rat Lungs
Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 20 - 30.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
W. Rong, A. V. Gourine, D. A. Cockayne, Z. Xiang, A. P. D. W. Ford, K. M. Spyer, and G. Burnstock
Pivotal Role of Nucleotide P2X2 Receptor Subunit of the ATP-Gated Ion Channel Mediating Ventilatory Responses to Hypoxia
J. Neurosci., December 10, 2003; 23(36): 11315 - 11321.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. W. Fu, C. A. Nurse, S. M. Farragher, and E. Cutz
Expression of functional nicotinic acetylcholine receptors in neuroepithelial bodies of neonatal hamster lung
Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1203 - L1212.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
P. P. Bertrand
ATP and Sensory Transduction in the Enteric Nervous System
Neuroscientist, August 1, 2003; 9(4): 243 - 260.
[Abstract] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
W. L. Neuhuber
Lung Sensors: Complex Functions Require Complex Structures
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 265 - 266.
[Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
I. Brouns, J. Van Genechten, H. Hayashi, M. Gajda, T. Gomi, G. Burnstock, J.-P. Timmermans, and D. Adriaensen
Dual Sensory Innervation of Pulmonary Neuroepithelial Bodies
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 275 - 285.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
I. Brouns, L. Van Nassauw, J. Van Genechten, M. Majewski, D. W. Scheuermann, J.-P. Timmermans, and D. Adriaensen
Triple Immunofluorescence Staining with Antibodies Raised in the Same Species to Study the Complex Innervation Pattern of Intrapulmonary Chemoreceptors
J. Histochem. Cytochem., April 1, 2002; 50(4): 575 - 582.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
B. A. Chizh and P. Illes
P2X Receptors and Nociception
Pharmacol. Rev., December 1, 2001; 53(4): 553 - 568.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society. 		  New Orleans Int'l Conf