help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jaspers, I.
Right arrow Articles by Reed, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jaspers, I.
Right arrow Articles by Reed, W.

Am. J. Respir. Cell Mol. Biol., Volume 23, Number 1, July, 2000 95-102

Vanadium-Induced kappa B-Dependent Transcription Depends upon Peroxide-Induced Activation of the p38 Mitogen-Activated Protein Kinase

Ilona Jaspers, James. M. Samet, Serpil Erzurum, and William Reed

Center for Environmental Medicine and Lung Biology, University of North Carolina School of Medicine, Chapel Hill; Human Studies Division, National Health and Environmental Effects Research Laboratory, Environmental Protection Agency, Research Triangle Park, North Carolina; and Cleveland Clinic Lerner Research Institute, Pulmonary and Critical Care Medicine/A90, Cleveland, Ohio

Activation of nuclear factor (NF)-kappa B and subsequent proinflammatory gene expression in human airway epithelial cells can be evoked by oxidative stress. In this study we examined signal transduction pathways activated by vanadyl sulfate (VIV)-induced oxidative stress in normal human bronchial epithelial cells. Both nuclear translocation of NF-kappa B and enhanced kappa B-dependent transcription induced by VIV were inhibited by overexpression of catalase, but not Cu,Zn superoxide dismutase (Cu,Zn-SOD), indicating that peroxides rather than superoxides initiated signaling. Catalase selectively blocked the response to VIV because it inhibited neither NF-kappa B translocation nor kappa B-dependent transcription evoked by the proinflammatory cytokine tumor necrosis factor (TNF)-alpha . The VIV-induced kappa B-dependent transcription was dependent upon activation of the p38 mitogen-activated protein kinase because overexpression of dominant-negative mutants of the p38 MAPK pathway inhibited VIV-induced kappa B-dependent transcription. This inhibition was not due to suppression of NF-kappa B nuclear translocation because NF-kappa B DNA binding was unaffected by the inhibition of p38 activity. Overexpression of catalase, but not Cu,Zn-SOD, inhibited p38 activation, indicating that peroxides activated p38. Catalase failed to block VIV- induced increases in phosphotyrosine levels, suggesting that the catalase-sensitive signaling components were independent of VIV-induced tyrosine phosphorylation. The data demonstrate that VIV-induced oxidative stress activates at least two distinct pathways, NF-kappa B nuclear translocation and p38-dependent transactivation of NF-kappa B, both of which are required to fully activate kappa B-dependent transcription. Moreover, VIV-induced oxidative stress activated these pathways in bronchial epithelial cells by upstream signaling cascades that were distinct at some level from those used by the proinflammatory cytokine TNF-alpha .




This article has been cited by other articles:


Home page
 Toxicol PatholHome page
J. C. Bonner
Lung Fibrotic Responses to Particle Exposure
Toxicol Pathol, January 1, 2007; 35(1): 148 - 153.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Turi, X. Wang, A. T. McKie, E. Nozik-Grayck, L. B. Mamo, K. Crissman, C. A. Piantadosi, and A. J. Ghio
Duodenal cytochrome b: a novel ferrireductase in airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L272 - L280.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y.-M. Kim, W. Reed, W. Wu, P. A. Bromberg, L. M. Graves, and J. M. Samet
Zn2+-induced IL-8 expression involves AP-1, JNK, and ERK activities in human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L1028 - L1035.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. Nakamichi, M. Saiki, M. Sawada, M. Takayama-Ito, Y. Yamamuro, K. Morimoto, and I. Kurane
Rabies Virus-Induced Activation of Mitogen-Activated Protein Kinase and NF-{kappa}B Signaling Pathways Regulates Expression of CXC and CC Chemokine Ligands in Microglia
J. Virol., September 15, 2005; 79(18): 11801 - 11812.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
I. Jaspers, J. M. Ciencewicki, W. Zhang, L. E. Brighton, J. L. Carson, M. A. Beck, and M. C. Madden
Diesel Exhaust Enhances Influenza Virus Infections in Respiratory Epithelial Cells
Toxicol. Sci., June 1, 2005; 85(2): 990 - 1002.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y.-M. Kim, W. Reed, A. G. Lenz, I. Jaspers, R. Silbajoris, H. S. Nick, and J. M. Samet
Ultrafine carbon particles induce interleukin-8 gene transcription and p38 MAPK activation in normal human bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L432 - L441.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Ingram, A. B. Rice, J. Santos, B. Van Houten, and J. C. Bonner
Vanadium-induced HB-EGF expression in human lung fibroblasts is oxidant dependent and requires MAP kinases
Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L774 - L782.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. S. Gilmour, I. Rahman, K. Donaldson, and W. MacNee
Histone acetylation regulates epithelial IL-8 release mediated by oxidative stress from environmental particles
Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L533 - L540.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. J. Ghio, H. B. Suliman, J. D. Carter, A. M. Abushamaa, and R. J. Folz
Overexpression of extracellular superoxide dismutase decreases lung injury after exposure to oil fly ash
Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L211 - L218.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. Wu, I. Jaspers, W. Zhang, L. M. Graves, and J. M. Samet
Role of Ras in metal-induced EGF receptor signaling and NF-kappa B activation in human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L1040 - L1048.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. P. BOWLER, J. ARCAROLI, J. D. CRAPO, A. ROSS, J. W. SLOT, and E. ABRAHAM
Extracellular Superoxide Dismutase Attenuates Lung Injury after Hemorrhage
Am. J. Respir. Crit. Care Med., July 15, 2001; 164(2): 290 - 294.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
I. Jaspers, W. Zhang, A. Fraser, J. M. Samet, and W. Reed
Hydrogen Peroxide Has Opposing Effects on IKK Activity and Ikappa Balpha Breakdown in Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., June 1, 2001; 24(6): 769 - 777.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Dai and A. Churg
Relationship of Fiber Surface Iron and Active Oxygen Species to Expression of Procollagen, PDGF-A, and TGF-{beta}1 in Tracheal Explants Exposed to Amosite Asbestos
Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 427 - 435.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society.