help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shukla, A.
Right arrow Articles by Mossman, B. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shukla, A.
Right arrow Articles by Mossman, B. T.

Am. J. Respir. Cell Mol. Biol., Volume 23, Number 2, August, 2000 182-187

Inhaled Particulate Matter Causes Expression of Nuclear Factor (NF)-kappa B-Related Genes and Oxidant-Dependent NF-kappa B Activation In Vitro

Arti Shukla, Cynthia Timblin, Kelly BeruBe, Terry Gordon, Willie McKinney, Kevin Driscoll, Pamela Vacek, and Brooke T. Mossman

Departments of Pathology and Biostatistics, University of Vermont, Burlington, Vermont; School of Biosciences, Cardiff University, Wales, United Kingdom; Environmental Medicine, New York University School of Medicine, Tuxedo, New York; and Cardiovascular Research, Procter & Gamble, Mason, Ohio

High levels of ambient air pollution are associated with exacerbation of asthma and respiratory morbidity, yet little is known concerning the mechanisms of inflammation and toxicity by components of inhaled particulate matter (PM). Brief inhalation of PM2.5 (particles of an aerodynamic diameter of < 2.5 microns) (300 µg/m3 air for 6 h followed by a period of 24 h in clean air) by either C3H/HeJ or C57/BL6 mice caused significant (P =< 0.05) increases in steady-state messenger RNA (mRNA) levels of a number of nuclear factor (NF)-kappa B-associated and/ or -regulated genes, including tumor necrosis factor-alpha and -beta , interleukin-6, interferon-gamma , and transforming growth factor-beta . Lung mRNA levels of lymphotoxin-beta and macrophage migration inhibitory factor were unchanged. In murine C10 alveolar cells and an NF-kappa B-luciferase reporter cell line, exposure to PM2.5 at noncytotoxic concentrations resulted in increases in transcriptional activation of NF-kappa B-dependent gene expression which were inhibited in the presence of catalase. Early and persistent increases in intracellular oxidants, as measured by flow cytometry and cell imaging using the oxidant probe 2'-7'-dichlorofluoroscin diacetate, were observed in epithelial cells exposed to PM2.5 and ultrafine carbon black particles. Studies here are the first to show NF-kappa B-related inflammatory and cytokine gene expression after inhalation of PM2.5 and oxidant-dependent induction of NF-kappa B activity by PM2.5 in pulmonary epithelial cells.




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
F. Alessandrini, I. Beck-Speier, D. Krappmann, I. Weichenmeier, S. Takenaka, E. Karg, B. Kloo, H. Schulz, T. Jakob, M. Mempel, et al.
Role of Oxidative Stress in Ultrafine Particle-induced Exacerbation of Allergic Lung Inflammation
Am. J. Respir. Crit. Care Med., June 1, 2009; 179(11): 984 - 991.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Zhao, P. V. Usatyuk, I. A. Gorshkova, D. He, T. Wang, L. Moreno-Vinasco, A. S. Geyh, P. N. Breysse, J. M. Samet, E. Wm. Spannhake, et al.
Regulation of COX-2 Expression and IL-6 Release by Particulate Matter in Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., January 1, 2009; 40(1): 19 - 30.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
I. Romieu, F. Castro-Giner, N. Kunzli, and J. Sunyer
Air pollution, oxidative stress and dietary supplementation: a review
Eur. Respir. J., January 1, 2008; 31(1): 179 - 197.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. A. Barlow, T. F. Barrett, A. Shukla, B. T. Mossman, and K. M. Lounsbury
Asbestos-mediated CREB phosphorylation is regulated by protein kinase A and extracellular signal-regulated kinases 1/2
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1361 - L1369.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Haegens, T. F. Barrett, J. Gell, A. Shukla, M. MacPherson, P. Vacek, M. E. Poynter, K. J. Butnor, Y. M. Janssen-Heininger, C. Steele, et al.
Airway Epithelial NF-{kappa}B Activation Modulates Asbestos-Induced Inflammation and Mucin Production In Vivo
J. Immunol., February 1, 2007; 178(3): 1800 - 1808.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. Shukla, K. M. Lounsbury, T. F. Barrett, J. Gell, M. Rincon, K. J. Butnor, D. J. Taatjes, G. S. Davis, P. Vacek, K. I. Nakayama, et al.
Asbestos-Induced Peribronchiolar Cell Proliferation and Cytokine Production Are Attenuated in Lungs of Protein Kinase C-{delta} Knockout Mice
Am. J. Pathol., January 1, 2007; 170(1): 140 - 151.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. Bhatnagar
Environmental Cardiology: Studying Mechanistic Links Between Pollution and Heart Disease
Circ. Res., September 29, 2006; 99(7): 692 - 705.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. A. Pulver-Kaste, C. A. Barlow, J. Bond, A. Watson, P. L. Penar, B. Tranmer, and K. M. Lounsbury
Ca2+ source-dependent transcription of CRE-containing genes in vascular smooth muscle
Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H97 - H105.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. Shukla, T. F. Barrett, K. I. Nakayama, K. Nakayama, B. T. Mossman, and K. M. Lounsbury
Transcriptional up-regulation of MMP12 and MMP13 by asbestos occurs via a PKC{delta}-dependent pathway in murine lung
FASEB J, May 1, 2006; 20(7): 997 - 999.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. A. Barlow, A. Shukla, B. T. Mossman, and K. M. Lounsbury
Oxidant-Mediated cAMP Response Element Binding Protein Activation: Calcium Regulation and Role in Apoptosis of Lung Epithelial Cells
Am. J. Respir. Cell Mol. Biol., January 1, 2006; 34(1): 7 - 14.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
Q. Sun, A. Wang, X. Jin, A. Natanzon, D. Duquaine, R. D. Brook, J.-G. S. Aguinaldo, Z. A. Fayad, V. Fuster, M. Lippmann, et al.
Long-term Air Pollution Exposure and Acceleration of Atherosclerosis and Vascular Inflammation in an Animal Model
JAMA, December 21, 2005; 294(23): 3003 - 3010.
[Abstract] [Full Text] [PDF]

Home page
 ERRHome page
D. Olivieri and E. Scoditti
Impact of environmental factors on lung defences
Eur. Respir. Rev., December 1, 2005; 14(95): 51 - 56.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y.-M. Kim, W. Reed, A. G. Lenz, I. Jaspers, R. Silbajoris, H. S. Nick, and J. M. Samet
Ultrafine carbon particles induce interleukin-8 gene transcription and p38 MAPK activation in normal human bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L432 - L441.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Shukla, T. Flanders, K. M. Lounsbury, and B. T. Mossman
The {gamma}-Glutamylcysteine Synthetase and Glutathione Regulate Asbestos-induced Expression of Activator Protein-1 Family Members and Activity
Cancer Res., November 1, 2004; 64(21): 7780 - 7786.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
C. R. Rhoden, J. Lawrence, J. J. Godleski, and B. Gonzalez-Flecha
N-Acetylcysteine Prevents Lung Inflammation After Short-Term Inhalation Exposure to Concentrated Ambient Particles
Toxicol. Sci., June 1, 2004; 79(2): 296 - 303.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
R. D. Brook, B. Franklin, W. Cascio, Y. Hong, G. Howard, M. Lipsett, R. Luepker, M. Mittleman, J. Samet, S. C. Smith Jr, et al.
Air Pollution and Cardiovascular Disease: A Statement for Healthcare Professionals From the Expert Panel on Population and Prevention Science of the American Heart Association
Circulation, June 1, 2004; 109(21): 2655 - 2671.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Blanchet, K. Ramgolam, A. Baulig, F. Marano, and A. Baeza-Squiban
Fine Particulate Matter Induces Amphiregulin Secretion by Bronchial Epithelial Cells
Am. J. Respir. Cell Mol. Biol., April 1, 2004; 30(4): 421 - 427.
[Abstract] [Full Text] [PDF]

Home page
 HeartHome page
H C Routledge, J G Ayres, and J N Townend
Why cardiologists should be interested in air pollution
Heart, December 1, 2003; 89(12): 1383 - 1388.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. KUMAR, S. LNU, R. MALYA, D. BARRON, J. MOORE, D. B. CORRY, and A. M. BORIEK
Mechanical stretch activates nuclear factor-kappaB, activator protein-1, and mitogen-activated protein kinases in lung parenchyma: implications in asthma
FASEB J, October 1, 2003; 17(13): 1800 - 1811.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Dai, C. Xie, R. Vincent, and A. Churg
Air Pollution Particles Produce Airway Wall Remodeling in Rat Tracheal Explants
Am. J. Respir. Cell Mol. Biol., September 1, 2003; 29(3): 352 - 358.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. E. Ramos-Nino, L. Scapoli, M. Martinelli, S. Land, and B. T. Mossman
Microarray Analysis and RNA Silencing Link fra-1 to cd44 and c-met Expression in Mesothelioma
Cancer Res., July 1, 2003; 63(13): 3539 - 3545.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Nemmar, M. F. Hoylaerts, P. H. M. Hoet, D. Dinsdale, T. Smith, H. Xu, J. Vermylen, and B. Nemery
Ultrafine Particles Affect Experimental Thrombosis in an In Vivo Hamster Model
Am. J. Respir. Crit. Care Med., October 1, 2002; 166(7): 998 - 1004.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
P. H. N. Saldiva, R. W. Clarke, B. A. Coull, R. C. Stearns, J. Lawrence, G. G. K. Murthy, E. Diaz, P. Koutrakis, H. Suh, A. Tsuda, et al.
Lung Inflammation Induced by Concentrated Ambient Air Particles Is Related to Particle Composition
Am. J. Respir. Crit. Care Med., June 15, 2002; 165(12): 1610 - 1617.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Dai, C. Xie, and A. Churg
Iron Loading Makes a Nonfibrogenic Model Air Pollutant Particle Fibrogenic In Rat Tracheal Explants
Am. J. Respir. Cell Mol. Biol., June 1, 2002; 26(6): 685 - 693.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. A. Jimenez, E. M. Drost, P. S. Gilmour, I. Rahman, F. Antonicelli, H. Ritchie, W. MacNee, and K. Donaldson
PM10-exposed macrophages stimulate a proinflammatory response in lung epithelial cells via TNF-alpha
Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L237 - L248.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. Hakonarson, E. Halapi, R. Whelan, J. Gulcher, K. Stefansson, and M. M. Grunstein
Association Between IL-1beta /TNF-alpha -Induced Glucocorticoid-Sensitive Changes in Multiple Gene Expression and Altered Responsiveness in Airway Smooth Muscle
Am. J. Respir. Cell Mol. Biol., December 1, 2001; 25(6): 761 - 771.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Shukla, C. R. Timblin, A. K. Hubbard, J. Bravman, and B. T. Mossman
Silica-induced Activation of c-Jun-NH2-Terminal Amino Kinases, Protracted Expression of the Activator Protein-1 Proto-Oncogene, fra-1, and S-Phase Alterations Are Mediated via Oxidative Stress
Cancer Res., March 1, 2001; 61(5): 1791 - 1795.
[Abstract] [Full Text]

Home page
 Hum Exp ToxicolHome page
R B Hefland, P E Schwarzel, B V Johansen, T Myran, N Uthus, and M Refsnes
Silica-induced cytokine release from A549 cells: importance of surface area versus size
Human and Experimental Toxicology, January 1, 2001; 20(1): 46 - 55.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. R. Sunil, A. J. Connor, Y. Guo, J. D. Laskin, and D. L. Laskin
Activation of type II alveolar epithelial cells during acute endotoxemia
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L872 - L880.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society. 		  ATS Coding and Billing Quarterly