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Am. J. Respir. Cell Mol. Biol., Volume 23, Number 2, August, 2000 182-187

Inhaled Particulate Matter Causes Expression of Nuclear Factor (NF)-kappa B-Related Genes and Oxidant-Dependent NF-kappa B Activation In Vitro

Arti Shukla, Cynthia Timblin, Kelly BeruBe, Terry Gordon, Willie McKinney, Kevin Driscoll, Pamela Vacek, and Brooke T. Mossman

Departments of Pathology and Biostatistics, University of Vermont, Burlington, Vermont; School of Biosciences, Cardiff University, Wales, United Kingdom; Environmental Medicine, New York University School of Medicine, Tuxedo, New York; and Cardiovascular Research, Procter & Gamble, Mason, Ohio

High levels of ambient air pollution are associated with exacerbation of asthma and respiratory morbidity, yet little is known concerning the mechanisms of inflammation and toxicity by components of inhaled particulate matter (PM). Brief inhalation of PM2.5 (particles of an aerodynamic diameter of < 2.5 microns) (300 µg/m3 air for 6 h followed by a period of 24 h in clean air) by either C3H/HeJ or C57/BL6 mice caused significant (P =< 0.05) increases in steady-state messenger RNA (mRNA) levels of a number of nuclear factor (NF)-kappa B-associated and/ or -regulated genes, including tumor necrosis factor-alpha and -beta , interleukin-6, interferon-gamma , and transforming growth factor-beta . Lung mRNA levels of lymphotoxin-beta and macrophage migration inhibitory factor were unchanged. In murine C10 alveolar cells and an NF-kappa B-luciferase reporter cell line, exposure to PM2.5 at noncytotoxic concentrations resulted in increases in transcriptional activation of NF-kappa B-dependent gene expression which were inhibited in the presence of catalase. Early and persistent increases in intracellular oxidants, as measured by flow cytometry and cell imaging using the oxidant probe 2'-7'-dichlorofluoroscin diacetate, were observed in epithelial cells exposed to PM2.5 and ultrafine carbon black particles. Studies here are the first to show NF-kappa B-related inflammatory and cytokine gene expression after inhalation of PM2.5 and oxidant-dependent induction of NF-kappa B activity by PM2.5 in pulmonary epithelial cells.




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