help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sueoka, N.
Right arrow Articles by Kurie, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sueoka, N.
Right arrow Articles by Kurie, J. M.

Am. J. Respir. Cell Mol. Biol., Volume 23, Number 3, September, 2000 297-303

Insulin-Like Growth Factor Binding Protein-6 Inhibits the Growth of Human Bronchial Epithelial Cells and Increases in Abundance with All-trans-Retinoic Acid Treatment

Naoko Sueoka, Ho-Young Lee, Garrett L. Walsh, Bingliang Fang, Lin Ji, Jack A. Roth, Ruth LaPushin, Waun K. Hong, Pinchas Cohen, and Jonathan M. Kurie

Departments of Thoracic/Head and Neck Medical Oncology, Thoracic and Cardiovascular Surgery, and Molecular Oncology, University of Texas-M. D. Anderson Cancer Center, Houston, Texas; and Department of Pediatrics, University of California at Los Angeles, Los Angeles, California

Retinoids are potent inhibitors of human bronchial epithelial (HBE) cell growth. Retinoids initiate signaling through activation of nuclear receptors, but the signal transduction pathways that mediate growth inhibition have not been defined. In this study, we investigated the expression of insulin-like growth factor (IGF)-binding protein (IGFBP)-6 as a potential mediator of retinoid actions. IGFBP-6 is a secreted glycoprotein that inhibits the bioavailability of IGFs, which are potent mitogens of HBE cells. IGFBP-6 was detected by immunohistochemical staining in the basal epithelial layer of human bronchial organ cultures, and all-trans-retinoic acid (t-RA) treatment increased the intensity of IGFBP-6 immunostaining. In primary cultures of HBE cells treated with t-RA, IGFBP-6 messenger RNA and protein levels increased within 6 and 24 h, respectively, and IGFBP-6 was detected in the conditioned media at 48 h. The effect of IGFBP-6 on HBE cell growth was investigated with a recombinant adenoviral vector, Ad5CMV-BP6, which expresses IGFBP-6 under the control of a cytomegalovirus promoter. IGFBP-6 overexpression induced a proliferative arrest of HBE cells with no evidence of apoptosis. These findings provide the first evidence that IGFBP-6 is expressed in the bronchial epithelium and that IGFBP-6 may contribute to the biologic effects of retinoids on HBE cells.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
I. P. Uray, Q. Shen, H.-S. Seo, H. Kim, W. W. Lamph, R. P. Bissonnette, and P. H. Brown
Rexinoid-induced Expression of IGFBP-6 Requires RAR{beta}-dependent Permissive Cooperation of Retinoid Receptors and AP-1
J. Biol. Chem., January 2, 2009; 284(1): 345 - 353.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
E. A. Acheampong, Z. Parveen, L. W. Muthoga, V. Wasmuth-Peroud, M. Kalayeh, A. Bashir, R. Diecidue, M. Mukhtar, and R. J. Pomerantz
Molecular Interactions of Human Immunodeficiency Virus Type 1 with Primary Human Oral Keratinocytes
J. Virol., July 1, 2005; 79(13): 8440 - 8453.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. A. Arslan, L. I. Gold, K. Mittal, T.-C. Suen, I. Belitskaya-Levy, M.-S. Tang, and P. Toniolo
Gene expression studies provide clues to the pathogenesis of uterine leiomyoma: new evidence and a systematic review
Hum. Reprod., April 1, 2005; 20(4): 852 - 863.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Daniely, G. Liao, D. Dixon, R. I. Linnoila, A. Lori, S. H. Randell, M. Oren, and A. M. Jetten
Critical role of p63 in the development of a normal esophageal and tracheobronchial epithelium
Am J Physiol Cell Physiol, July 1, 2004; 287(1): C171 - C181.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
D. Newman, M. Sakaue, J. S. Koo, K.-S. Kim, S. J. Baek, T. Eling, and A. M. Jetten
Differential Regulation of Nonsteroidal Anti-Inflammatory Drug-Activated Gene in Normal Human Tracheobronchial Epithelial and Lung Carcinoma Cells by Retinoids
Mol. Pharmacol., March 1, 2003; 63(3): 557 - 564.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H.-Y. Lee, K.-H. Chun, B. Liu, S. A. Wiehle, R. J. Cristiano, W. K. Hong, P. Cohen, and J. M. Kurie
Insulin-like Growth Factor Binding Protein-3 Inhibits the Growth of Non-Small Cell Lung Cancer
Cancer Res., June 1, 2002; 62(12): 3530 - 3537.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. A. Shoubridge, C.-B. Steeb, and L. C. Read
IGFBP mRNA expression in small intestine of rat during postnatal development
Am J Physiol Gastrointest Liver Physiol, December 1, 2001; 281(6): G1378 - G1384.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society. 		  CCM abstracts