help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hirst, S. J.
Right arrow Articles by Lee, T. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hirst, S. J.
Right arrow Articles by Lee, T. H.

Am. J. Respir. Cell Mol. Biol., Volume 23, Number 3, September, 2000 335-344

Differential Effects of Extracellular Matrix Proteins on Human Airway Smooth Muscle Cell Proliferation and Phenotype

Stuart J. Hirst, Charles H. C. Twort, and Tak H. Lee

Department of Respiratory Medicine and Allergy, The Guy's, King's, and St. Thomas' School of Medicine, King's College London, Guy's Hospital Campus, London, United Kingdom

Mature airway smooth muscle cells are characterized by a low proliferative index and expression of contractile marker proteins such as smooth muscle alpha -actin (sm-alpha -actin), calponin, and smooth muscle myosin heavy chain (sm-MHC). In the present study, defined extracellular matrix (ECM) components were examined on the proliferative and phenotypic status of mitogen-stimulated, cultured human airway smooth muscle cells. The results demonstrate that although cells adhered and spread on plates precoated with (1 to 100 µg/ml) of fibronectin (FN), collagen I (Col I), laminin (LN), or Matrigel, their subsequent proliferative response varied qualitatively. FN and Col I enhanced proliferation in response to either platelet-derived growth factor (PDGF)-BB or alpha -thrombin, compared with cells on plastic. LN, however, reduced mitogen-stimulated proliferation. A similar reduction was found in cells cultured on Matrigel. The effect of ECM substrates on contractile phenotype was determined by examining cellular expression of sm-alpha -actin, sm-MHC, and calponin using immunocytochemical and flow cytometric methods. Approximately 75% of PDGF-BB-stimulated cells, cultured on LN or Matrigel, expressed sm-alpha -actin, calponin, and sm-MHC, but only 8 to 10% stained for the Ki67 nuclear antigen proliferation marker. In contrast, more than 75% of cells cultured on FN or Col I were positive for Ki67 antigen, but only 20% were positive for contractile proteins. Flow cytometric analysis of sm-alpha -actin and DNA content confirmed the immunocytochemical findings and showed that the observed reduction in sm-alpha -actin content after culture on FN or Col I, compared with LN and Matrigel, occurred in the majority of the cell population, supporting bidirectional phenotype modulation. Overall, the data suggest that ECM substrates modulate both proliferation and phenotype of human airway smooth muscle cells in culture.




This article has been cited by other articles:


Home page
 Eur Respir JHome page
S. Zuyderduyn, M. B. Sukkar, A. Fust, S. Dhaliwal, and J. K. Burgess
Treating asthma means treating airway smooth muscle cells
Eur. Respir. J., August 1, 2008; 32(2): 265 - 274.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
B. B. Araujo, M. Dolhnikoff, L. F. F. Silva, J. Elliot, J. H. N. Lindeman, D. S. Ferreira, A. Mulder, H. A. P. Gomes, S. M. Fernezlian, A. James, et al.
Extracellular matrix components and regulators in the airway smooth muscle in asthma
Eur. Respir. J., July 1, 2008; 32(1): 61 - 69.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
Z. Gong and L. E. Niklason
Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs)
FASEB J, June 1, 2008; 22(6): 1635 - 1648.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. L. D'Antoni, C. Torregiani, P. Ferraro, M.-C. Michoud, B. Mazer, J. G. Martin, and M. S. Ludwig
Effects of decorin and biglycan on human airway smooth muscle cell proliferation and apoptosis
Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L764 - L771.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
J. G. Martin and T. Jo
Genetic Differences in Airway Smooth Muscle Function
Proceedings of the ATS, January 1, 2008; 5(1): 73 - 79.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
A. J. Halayko, T. Tran, and R. Gosens
Phenotype and Functional Plasticity of Airway Smooth Muscle: Role of Caveolae and Caveolins
Proceedings of the ATS, January 1, 2008; 5(1): 80 - 88.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
T. Tran, K. Ens-Blackie, E. S. Rector, G. L. Stelmack, K. D. McNeill, G. Tarone, W. T. Gerthoffer, H. Unruh, and A. J. Halayko
Laminin-Binding Integrin {alpha}7 Is Required for Contractile Phenotype Expression by Human Airway Myocytes
Am. J. Respir. Cell Mol. Biol., December 1, 2007; 37(6): 668 - 680.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. G. J. Dekkers, D. Schaafsma, S. A. Nelemans, J. Zaagsma, and H. Meurs
Extracellular matrix proteins differentially regulate airway smooth muscle phenotype and function
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1405 - L1413.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
S. S. An, T. R. Bai, J. H. T. Bates, J. L. Black, R. H. Brown, V. Brusasco, P. Chitano, L. Deng, M. Dowell, D. H. Eidelman, et al.
Airway smooth muscle dynamics: a common pathway of airway obstruction in asthma
Eur. Respir. J., May 1, 2007; 29(5): 834 - 860.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Henderson, L. J. Markwick, S. R. Elshaw, A. M. Freyer, A. J. Knox, and S. R. Johnson
Collagen I and thrombin activate MMP-2 by MMP-14-dependent and -independent pathways: implications for airway smooth muscle migration
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L1030 - L1038.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
E. D. Fixman, A. Stewart, and J. G. Martin
Basic mechanisms of development of airway structural changes in asthma
Eur. Respir. J., February 1, 2007; 29(2): 379 - 389.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
L. Pini, Q. Hamid, J. Shannon, L. Lemelin, R. Olivenstein, P. Ernst, C. Lemiere, J. G. Martin, and M. S. Ludwig
Differences in proteoglycan deposition in the airways of moderate and severe asthmatics
Eur. Respir. J., January 1, 2007; 29(1): 71 - 77.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. J. Ammit, L. M. Moir, B. G. Oliver, J. M. Hughes, H. Alkhouri, Q. Ge, J. K. Burgess, J. L. Black, and M. Roth
Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L199 - L206.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. Bachar, M. Adner, and L.-O. Cardell
Toll-like receptor activation in airway smooth muscle: dual actions via separate MAPK pathways
Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L322 - L323.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
V. Chan, J. K. Burgess, J. C. Ratoff, B. J. O'Connor, A. Greenough, T. H. Lee, and S. J. Hirst
Extracellular Matrix Regulates Enhanced Eotaxin Expression in Asthmatic Airway Smooth Muscle Cells
Am. J. Respir. Crit. Care Med., August 15, 2006; 174(4): 379 - 385.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
K. F. Chung
The Role of Airway Smooth Muscle in the Pathogenesis of Airway Wall Remodeling in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, November 1, 2005; 2(4): 347 - 354.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
V. Govindaraju, J. G. Martin, K. Maghni, P. Ferraro, and M.-C. Michoud
The Effects of Extracellular Purines and Pyrimidines on Human Airway Smooth Muscle Cells
J. Pharmacol. Exp. Ther., November 1, 2005; 315(2): 941 - 948.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
L. Yu, D. A. Quinn, H. G. Garg, and C. A. Hales
Cyclin-Dependent Kinase Inhibitor p27Kip1, But Not p21WAF1/Cip1, Is Required for Inhibition of Hypoxia-Induced Pulmonary Hypertension and Remodeling by Heparin in Mice
Circ. Res., October 28, 2005; 97(9): 937 - 945.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
U. Oltmanns, M. B. Sukkar, S. Xie, M. John, and K. F. Chung
Induction of Human Airway Smooth Muscle Apoptosis by Neutrophils and Neutrophil Elastase
Am. J. Respir. Cell Mol. Biol., April 1, 2005; 32(4): 334 - 341.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Q. Peng, D. Lai, T. T.-B. Nguyen, V. Chan, T. Matsuda, and S. J. Hirst
Multiple {beta}1 Integrins Mediate Enhancement of Human Airway Smooth Muscle Cytokine Secretion by Fibronectin and Type I Collagen
J. Immunol., February 15, 2005; 174(4): 2258 - 2264.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T. T.-B. Nguyen, J. P. T. Ward, and S. J. Hirst
{beta}1-Integrins Mediate Enhancement of Airway Smooth Muscle Proliferation by Collagen and Fibronectin
Am. J. Respir. Crit. Care Med., February 1, 2005; 171(3): 217 - 223.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A.G. Stewart
Emigration and immigration of mesenchymal cells: a multicultural airway wall
Eur. Respir. J., October 1, 2004; 24(4): 515 - 517.
[Full Text] [PDF]

Home page
 Eur Respir JHome page
K. Parameswaran, K. Radford, J. Zuo, L.J. Janssen, P.M. O'Byrne, and P.G. Cox
Extracellular matrix regulates human airway smooth muscle cell migration
Eur. Respir. J., October 1, 2004; 24(4): 545 - 551.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. M. Freyer, C. K. Billington, R. B. Penn, and I. P. Hall
Extracellular Matrix Modulates {beta}2-Adrenergic Receptor Signaling in Human Airway Smooth Muscle Cells
Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 440 - 445.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Yu, I. Fantozzi, C. V. Remillard, J. W. Landsberg, N. Kunichika, O. Platoshyn, D. D. Tigno, P. A. Thistlethwaite, L. J. Rubin, and J. X.-J. Yuan
Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension
PNAS, September 21, 2004; 101(38): 13861 - 13866.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Y. Cho, M. Miller, K. J. Baek, J. W. Han, J. Nayar, M. Rodriguez, S. Y. Lee, K. McElwain, S. McElwain, E. Raz, et al.
Immunostimulatory DNA Inhibits Transforming Growth Factor-{beta} Expression and Airway Remodeling
Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 651 - 661.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
D. C. Grootendorst and K. F. Rabe
Mechanisms of Bronchial Hyperreactivity in Asthma and Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, April 1, 2004; 1(2): 77 - 87.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. Johansson and K. Persson
Phenotypic modulation of cultured bladder smooth muscle cells and the expression of inducible nitric oxide synthase
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2004; 286(4): R642 - R648.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. S. Kazi, S. Lotfi, E. A. Goncharova, O. Tliba, Y. Amrani, V. P. Krymskaya, and A. L. Lazaar
Vascular endothelial growth factor-induced secretion of fibronectin is ERK dependent
Am J Physiol Lung Cell Mol Physiol, March 1, 2004; 286(3): L539 - L545.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
L. Benayoun, A. Druilhe, M.-C. Dombret, M. Aubier, and M. Pretolani
Airway Structural Alterations Selectively Associated with Severe Asthma
Am. J. Respir. Crit. Care Med., May 15, 2003; 167(10): 1360 - 1368.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. M. Moir, S.-Y. Leung, P. R. Eynott, C. G. McVicker, J. P. T. Ward, K. F. Chung, and S. J. Hirst
Repeated allergen inhalation induces phenotypic modulation of smooth muscle in bronchioles of sensitized rats
Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L148 - L159.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. M. Freyer, S. R. Johnson, and I. P. Hall
Effects of Growth Factors and Extracellular Matrix on Survival of Human Airway Smooth Muscle Cells
Am. J. Respir. Cell Mol. Biol., November 1, 2001; 25(5): 569 - 576.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. J. Ammit and R. A. Panettieri Jr.
Signal Transduction in Smooth Muscle: Invited Review: The circle of life: cell cycle regulation in airway smooth muscle
J Appl Physiol, September 1, 2001; 91(3): 1431 - 1437.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. J. Yarwood and J. R. Woodgett
Extracellular matrix composition determines the transcriptional response to epidermal growth factor receptor activation
PNAS, March 29, 2001; (2001) 81069098.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
A. J. Halayko and J. Solway
Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Molecular mechanisms of phenotypic plasticity in smooth muscle cells
J Appl Physiol, January 1, 2001; 90(1): 358 - 368.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. J. Yarwood and J. R. Woodgett
Extracellular matrix composition determines the transcriptional response to epidermal growth factor receptor activation
PNAS, April 10, 2001; 98(8): 4472 - 4477.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society.