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Am. J. Respir. Cell Mol. Biol., Volume 23, Number 4, October, 2000 492-498

Endothelial Sulfated Sialyl Lewis x Glycans, Putative L-Selectin Ligands, Are Preferentially Expressed in Bronchial Asthma but Not in Other Chronic Inflammatory Lung Diseases

Sanna Toppila, Timo Paavonen, Annika Laitinen, Lauri A. Laitinen, and Risto Renkonen

Department of Bacteriology and Immunology; Department of Pathology, Haartman Institute; Department of Anatomy, Institute of Biomedicine, University of Helsinki; Department of Medicine; Laboratory Diagnostics, Helsinki University Central Hospital, Helsinki, Finland

Lymphocyte infiltrate is a hallmark of inflammatory responses. We have previously shown that de novo-induced endothelial sialyl Lewis x (sLex) expression guides lymphocytes in an L-selectin-dependent manner to sites of acute organ transplant rejections. In this research, we have analyzed five groups of chronic lung inflammations to determine the presence of properly glycosylated, i.e., sulfated, sLex-decorated, L-selectin ligands. Two anti-sLex (2F3 and HECA-452) and one anti-6- and/or 6'-sulfated and/or 6,6'-bisulfated (MECA-79) monoclonal antibodies (mAbs) were used. The control lung specimens did not express L-selectin ligands on endothelium. In contrast, the endothelial staining intensity and the number of positive peribronchial venules and capillaries with mAbs 2F3, HECA-452, and MECA-79 were significantly greater in bronchial biopsies from patients with asthma compared with normal specimens (P < 0.003). However, no significant increase of peribronchial endothelial reactivity with these antibodies was observed in adult respiratory distress syndrome, chronic bronchitis, fibrosing alveolitis, and granulomatous inflammation compared with controls. These data suggest that sulfated sLex glycans, acting putatively as ligands for L-selectin, could be instrumental in lymphocyte extravasation into human peribronchial lung tissue during asthma, but not so important in several other inflammatory lung diseases.




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