help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coyne, C. B.
Right arrow Articles by Johnson, L. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coyne, C. B.
Right arrow Articles by Johnson, L. G.

Am. J. Respir. Cell Mol. Biol., Volume 23, Number 5, November, 2000 602-609

Enhanced Epithelial Gene Transfer by Modulation of Tight Junctions with Sodium Caprate

Carolyn B. Coyne, Miriam M. Kelly, Richard C. Boucher, and Larry G. Johnson

Departments of Pharmacology and Medicine, and Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

The airway epithelium is resistant to infection by gene transfer vectors when infected from the luminal surface. One strategy for enhancing airway epithelial gene transfer is to modify paracellular permeability, thereby permitting the diffusion of vectors to the basolateral surface, where uptake receptors are expressed. We investigated the ability of a medium-chain fatty acid known to enhance drug absorption, sodium caprate (C10), to increase airway paracellular permeability in comparison with ethyleneglycol-bis-(beta -aminoethyl ether)-N,N'-tetraacetic acid (EGTA). Apical application of C10 decreased transepithelial resistance by > 90% within minutes, whereas EGTA required an hour or more to produce a similar effect. C10 increased mannitol and dextran permeability by sevenfold, as compared with a twofold increase produced by EGTA. A greater enhancement of adenoviral lacZ gene transfer was mediated by C10 (50-fold over controls) than by EGTA (10-fold over controls). This correlated with a significant enhancement of adenoviral CFTR-mediated correction of Cl- transport in polarized human airway epithelial (HAE) cells from cystic fibrosis (CF) patients. Confocal microscopy revealed a redistribution of claudin-1 following C10 but not EGTA treatment as a possible mechanism of gene-transfer enhancement by C10. These data suggest that C10 may be a better agent for enhancing gene transfer than is EGTA, and that this effect occurs through disruption of claudin-1.




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
X.-b. Chang, A. Mengos, Y.-x. Hou, L. Cui, T. J. Jensen, A. Aleksandrov, J. R. Riordan, and M. Gentzsch
Role of N-linked oligosaccharides in the biosynthetic processing of the cystic fibrosis membrane conductance regulator
J. Cell Sci., September 1, 2008; 121(17): 2814 - 2823.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Pan, Y. You, T. Huang, and S. L. Brody
RhoA-mediated apical actin enrichment is required for ciliogenesis and promoted by Foxj1
J. Cell Sci., June 1, 2007; 120(11): 1868 - 1876.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. R. Hallows, A. C. Fitch, C. A. Richardson, P. R. Reynolds, J. P. Clancy, P. C. Dagher, L. A. Witters, J. K. Kolls, and J. M. Pilewski
Up-regulation of AMP-activated Kinase by Dysfunctional Cystic Fibrosis Transmembrane Conductance Regulator in Cystic Fibrosis Airway Epithelial Cells Mitigates Excessive Inflammation
J. Biol. Chem., February 17, 2006; 281(7): 4231 - 4241.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
R. S. Everett, M. K. Vanhook, N. Barozzi, I. Toth, and L. G. Johnson
Specific Modulation of Airway Epithelial Tight Junctions by Apical Application of an Occludin Peptide
Mol. Pharmacol., February 1, 2006; 69(2): 492 - 500.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
J Hu, X Zhang, X Dong, L Collins, G J Sawyer, and J W Fabre
A remarkable permeability of canalicular tight junctions might facilitate retrograde, non-viral gene delivery to the liver via the bile duct
Gut, October 1, 2005; 54(10): 1473 - 1479.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. L. Sinn, A. J. Shah, M. D. Donovan, and P. B. McCray Jr.
Viscoelastic Gel Formulations Enhance Airway Epithelial Gene Transfer with Viral Vectors
Am. J. Respir. Cell Mol. Biol., May 1, 2005; 32(5): 404 - 410.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. Zhang, A. Bukreyev, C. I. Thompson, B. Watson, M. E. Peeples, P. L. Collins, and R. J. Pickles
Infection of Ciliated Cells by Human Parainfluenza Virus Type 3 in an In Vitro Model of Human Airway Epithelium
J. Virol., January 15, 2005; 79(2): 1113 - 1124.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. R. Stonebraker, D. Wagner, R. W. Lefensty, K. Burns, S. J. Gendler, J. M. Bergelson, R. C. Boucher, W. K. O'Neal, and R. J. Pickles
Glycocalyx Restricts Adenoviral Vector Access to Apical Receptors Expressed on Respiratory Epithelium In Vitro and In Vivo: Role for Tethered Mucins as Barriers to Lumenal Infection
J. Virol., December 15, 2004; 78(24): 13755 - 13768.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
R. J. Pickles
Physical and Biological Barriers to Viral Vector-mediated Delivery of Genes to the Airway Epithelium
Proceedings of the ATS, December 1, 2004; 1(4): 302 - 308.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. R. Broughman, R. M. Brandt, C. Hastings, T. Iwamoto, J. M. Tomich, and B. D. Schultz
Channel-forming peptide modulates transepithelial electrical conductance and solute permeability
Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1312 - C1323.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
U. E. Schwab, C. M. P. Ribeiro, H. Neubauer, and R. C. Boucher
Role of Actin Filament Network in Burkholderia multivorans Invasion in Well-Differentiated Human Airway Epithelia
Infect. Immun., November 1, 2003; 71(11): 6607 - 6609.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. B. Coyne, T. M. Gambling, R. C. Boucher, J. L. Carson, and L. G. Johnson
Role of claudin interactions in airway tight junctional permeability
Am J Physiol Lung Cell Mol Physiol, November 1, 2003; 285(5): L1166 - L1178.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
C. B. Coyne, C. M. P. Ribeiro, R. C. Boucher, and L. G. Johnson
Acute Mechanism of Medium Chain Fatty Acid-Induced Enhancement of Airway Epithelial Permeability
J. Pharmacol. Exp. Ther., May 1, 2003; 305(2): 440 - 450.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. R. Hallows, G. P. Kobinger, J. M. Wilson, L. A. Witters, and J. K. Foskett
Physiological modulation of CFTR activity by AMP-activated protein kinase in polarized T84 cells
Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1297 - C1308.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Perez-Vilar, J. K. Sheehan, and S. H. Randell
Making More MUCS
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 267 - 270.
[Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M.-C. Nlend, R. J. Bookman, G. E. Conner, and M. Salathe
Regulator of G-Protein Signaling Protein 2 Modulates Purinergic Calcium and Ciliary Beat Frequency Responses in Airway Epithelia
Am. J. Respir. Cell Mol. Biol., October 1, 2002; 27(4): 436 - 445.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. M. Pilewski
Gene Therapy for Airway Diseases: Continued Progress toward Identifying and Overcoming Barriers to Efficiency
Am. J. Respir. Cell Mol. Biol., August 1, 2002; 27(2): 117 - 121.
[Full Text] [PDF]

Home page
 J. Virol.Home page
L. Zhang, M. E. Peeples, R. C. Boucher, P. L. Collins, and R. J. Pickles
Respiratory Syncytial Virus Infection of Human Airway Epithelial Cells Is Polarized, Specific to Ciliated Cells, and without Obvious Cytopathology
J. Virol., May 3, 2002; 76(11): 5654 - 5666.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society.