help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Man, Y.
Right arrow Articles by West, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Man, Y.
Right arrow Articles by West, M. R.

Am. J. Respir. Cell Mol. Biol., Volume 23, Number 5, November, 2000 610-617

Loss of Epithelial Integrity Resulting from E-Cadherin Dysfunction Predisposes Airway Epithelial Cells to Adenoviral Infection

Yim Man, Vincent J. Hart, Christopher J. A. Ring, Shahin Sanjar, and Michael R. West

Cell Biology Department and Clinical Virology Department, Medicines Research Centre, GlaxoWellcome, Stevenage, United Kingdom; and Respiratory Therapeutic Development, GlaxoWellcome, Inc., Research Triangle Park, North Carolina

Epithelial intercellular adhesion is fundamental to the formation of the airway epithelial protective barrier. In this respect, cadherins are important because these adhesion molecules regulate formation and maintenance of epithelial intercellular junctions. To study the importance of airway epithelial integrity in determining susceptibility to virus infection, we used a replication-incompetent adenovirus, RAd35, and an E-cadherin specific function-blocking antibody, SHE78-7, to disrupt intercellular contacts in human bronchial epithelial cell line 16HBE14o- and primary bronchial epithelial cells. After exposure of 16HBE14o- cell cultures to SHE78-7, disruption of the transepithelial permeability barrier was indicated by a loss of transepithelial electrical resistance and an associated increase of mannitol, inulin, and dextran paracellular flux. Subsequent exposure of SHE78-7-treated cell cultures to RAd35 showed a remarkable increase in adenoviral infection as assessed by beta -galactosidase reporter gene expression. In cultures exposed to SHE78-7, disruption of E-cadherin function resulted in infection equivalent to that in control cultures using 16-fold lower viral titers. These studies show that manipulation of E-cadherin function provides a specific means of altering epithelial integrity that in turn determines resistance of airway epithelia to adenoviral infection.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Liu, X. Guo, J. N. Rao, T. Zou, L. Xiao, T. Yu, J. A. Timmons, D. J. Turner, and J.-Y. Wang
Polyamines regulate E-cadherin transcription through c-Myc modulating intestinal epithelial barrier function
Am J Physiol Cell Physiol, April 1, 2009; 296(4): C801 - C810.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Salter, K. Biggadike, J. L. Matthews, M. R. West, M. V. Haase, S. N. Farrow, I. J. Uings, and D. W. Gray
Pharmacological properties of the enhanced-affinity glucocorticoid fluticasone furoate in vitro and in an in vivo model of respiratory inflammatory disease
Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L660 - L667.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. L. Humlicek, L. J. Manzel, C. L. Chin, L. Shi, K. J. D. A. Excoffon, M. C. Winter, D. M. Shasby, and D. C. Look
Paracellular Permeability Restricts Airway Epithelial Responses to Selectively Allow Activation by Mediators at the Basolateral Surface
J. Immunol., May 15, 2007; 178(10): 6395 - 6403.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
P. P Y Lie, W. Xia, C. Q F Wang, D. D Mruk, H. H N Yan, C.-h. Wong, W. M Lee, and C Y. Cheng
Dynamin II interacts with the cadherin- and occludin-based protein complexes at the blood-testis barrier in adult rat testes
J. Endocrinol., December 1, 2006; 191(3): 571 - 586.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Kim, A. J. Schein, and J. A. Nadel
E-cadherin promotes EGFR-mediated cell differentiation and MUC5AC mucin expression in cultured human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1049 - L1060.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. H. N. Yan and C. Y. Cheng
Blood-testis barrier dynamics are regulated by an engagement/disengagement mechanism between tight and adherens junctions via peripheral adaptors
PNAS, August 16, 2005; 102(33): 11722 - 11727.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
X.-F. Wang, J. Z. Cui, S. S. Prasad, and J. A. Matsubara
Altered Gene Expression of Angiogenic Factors Induced by Calcium-Mediated Dissociation of Retinal Pigment Epithelial Cells
Invest. Ophthalmol. Vis. Sci., April 1, 2005; 46(4): 1508 - 1515.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
X. Guo, J. N. Rao, L. Liu, T.-T. Zou, D. J. Turner, B. L. Bass, and J.-Y. Wang
Regulation of adherens junctions and epithelial paracellular permeability: a novel function for polyamines
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1174 - C1187.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. M. Evans, D. I. Blyth, T. Wong, S. Sanjar, and M. R. West
Decreased Distribution of Lung Epithelial Junction Proteins after Intratracheal Antigen or Lipopolysaccharide Challenge: Correlation with Neutrophil Influx and Levels of BALF sE-Cadherin
Am. J. Respir. Cell Mol. Biol., October 1, 2002; 27(4): 446 - 454.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society. 		  Red In Translatin