help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, C. G.
Right arrow Articles by Elias, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, C. G.
Right arrow Articles by Elias, J. A.

Am. J. Respir. Cell Mol. Biol., Volume 23, Number 5, November, 2000 662-669

Respiratory Syncytial Virus Stimulation of Vascular Endothelial Cell Growth Factor/Vascular Permeability Factor

Chun Geun Lee, Ho Joo Yoon, Zhou Zhu, Holger Link, Zhongde Wang, Jack M. Gwaltney Jr., Marie Landry, and Jack A. Elias

Section of Pulmonary and Critical Care Medicine, Department of Internal Medicine; Section of Respiratory Medicine, Department of Pediatrics; Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut; and Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville, Virginia

We hypothesized that respiratory syncytial virus (RSV)-induced pathologies could be mediated, in part, by vascular active cytokines elaborated during virus infection. To address this hypothesis, we determined whether RSV stimulated vascular endothelial cell growth factor (VEGF)/vascular permeability factor (VPF) elaboration in vitro. Supernatants from unstimulated A549 cells and normal human bronchial epithelial cells contained modest levels of VEGF. In contrast, supernatants from RSV-infected cells contained elevated levels of VEGF/VPF. This stimulation was seen after as little as 2 h, was still prominent after 48 h, and, by immunoblot, was specific for the 165- and 121-amino acid isoforms of VEGF/VPF. It was not associated with significant cell cytotoxicity or alterations in VEGF messenger RNA. It did, however, require new protein biosynthesis. In accordance with these findings, the 165- and 121-amino acid isoforms of VEGF/VPF were also found in the nasal washings from patients with RSV infections. These studies demonstrate that RSV is a potent stimulator of VEGF/VPF elaboration and that, in vitro, this stimulation is mediated via a noncytotoxic translational and/or post-translational biosynthetic mechanism. VEGF/VPF may play an important role in the pathogenesis of RSV-induced disorders.




This article has been cited by other articles:


Home page
 J. Virol.Home page
L.-W. Qian, W. Greene, F. Ye, and S.-J. Gao
Kaposi's Sarcoma-Associated Herpesvirus Disrupts Adherens Junctions and Increases Endothelial Permeability by Inducing Degradation of VE-Cadherin
J. Virol., December 1, 2008; 82(23): 11902 - 11912.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Singh, K. L. McCann, and F. Imani
MAPK and heat shock protein 27 activation are associated with respiratory syncytial virus induction of human bronchial epithelial monolayer disruption
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L436 - L445.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. V. Avdalovic, L. F. Putney, E. S. Schelegle, L. Miller, J. L. Usachenko, N. K. Tyler, C. G. Plopper, L. J. Gershwin, and D. M. Hyde
Vascular Remodeling Is Airway Generation-Specific in a Primate Model of Chronic Asthma
Am. J. Respir. Crit. Care Med., November 15, 2006; 174(10): 1069 - 1076.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Bhandari, R. Choo-Wing, S. P. Chapoval, C. G. Lee, C. Tang, Y. K. Kim, B. Ma, P. Baluk, M. I. Lin, D. M. McDonald, et al.
Essential role of nitric oxide in VEGF-induced, asthma-like angiogenic, inflammatory, mucus, and physiologic responses in the lung
PNAS, July 18, 2006; 103(29): 11021 - 11026.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
C. G. Lee, H.-R. Kang, R. J. Homer, G. Chupp, and J. A. Elias
Transgenic Modeling of Transforming Growth Factor-{beta}1: Role of Apoptosis in Fibrosis and Alveolar Remodeling.
Proceedings of the ATS, July 1, 2006; 3(5): 418 - 423.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Mura, C. C. dos Santos, D. Stewart, and M. Liu
Vascular endothelial growth factor and related molecules in acute lung injury
J Appl Physiol, November 1, 2004; 97(5): 1605 - 1617.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. M. Kilani, K. A. Mohammed, N. Nasreen, J. A. Hardwick, M. H. Kaplan, R. S. Tepper, and V. B. Antony
Respiratory Syncytial Virus Causes Increased Bronchial Epithelial Permeability
Chest, July 1, 2004; 126(1): 186 - 191.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
A Ohwada, Y Yoshioka, K Iwabuchi, I Nagaoka, T Dambara, and Y Fukuchi
VEGF regulates the proliferation of acid-exposed alveolar lining epithelial cells
Thorax, April 1, 2003; 58(4): 328 - 332.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
D. B. Jacoby
Virus-Induced Asthma Attacks
JAMA, February 13, 2002; 287(6): 755 - 761.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society. 		  ATS Coding and Billing Quarterly