help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Girón-Calle, J.
Right arrow Articles by Forman, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Girón-Calle, J.
Right arrow Articles by Forman, H. J.

Am. J. Respir. Cell Mol. Biol., Volume 23, Number 6, December, 2000 748-754

Phospholipase D and Priming of the Respiratory Burst by H2O2 in NR8383 Alveolar Macrophages

Julio Girón-Calle and Henry Jay Forman

Department of Environmental Health Sciences, School of Public Health, and Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama

Previous investigation showed that preincubation within a range of nontoxic H2O2 concentrations enhanced subsequently stimulated superoxide production by rat alveolar macrophages in response to various stimuli. In the present study, the NR8383 rat alveolar macrophage cell line was used to further investigate the priming effect of H2O2. Using nitroblue tetrazolium, which formed an insoluble formazan when reduced by superoxide, modulation of the respiratory burst was visualized in a cell population exposed to a concentration gradient of H2O2 before stimulation. This model system illustrates how H2O2 may constitute a signaling molecule for a feed-forward regulation of the respiratory burst during inflammation. n-Butanol, which allows consumption of phosphatidic acid by the transphosphatidylation reaction, and propanolol, which inhibits phosphatidic acid phosphohydrolase, were used to investigate the possible involvement of phospholipase D in this phenomenon. These two agents were found to inhibit the basal adenosine diphosphate-stimulated respiratory burst. Inhibition of the H2O2-enhanced respiratory burst was equally or slightly less effective when expressed as percentage of controls. Furthermore, phospholipase D was not activated by H2O2 concentrations that enhance superoxide production. Thus, phospholipase D does not mediate the enhancement of the respiratory burst by H2O2, although it may be activated by high concentrations of this hydroperoxide.




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. Liu, H. Zhang, and H. J. Forman
Silica Induces Macrophage Cytokines through Phosphatidylcholine-Specific Phospholipase C with Hydrogen Peroxide
Am. J. Respir. Cell Mol. Biol., May 1, 2007; 36(5): 594 - 599.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. J. Cortez, C. A. Lyman, S. Kottilil, H. S. Kim, E. Roilides, J. Yang, B. Fullmer, R. Lempicki, and T. J. Walsh
Functional Genomics of Innate Host Defense Molecules in Normal Human Monocytes in Response to Aspergillus fumigatus
Infect. Immun., April 1, 2006; 74(4): 2353 - 2365.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
C. Steele, L. Marrero, S. Swain, A. G. Harmsen, M. Zheng, G. D. Brown, S. Gordon, J. E. Shellito, and J. K. Kolls
Alveolar Macrophage-mediated Killing of Pneumocystis carinii f. sp. muris Involves Molecular Recognition by the Dectin-1 {beta}-Glucan Receptor
J. Exp. Med., December 1, 2003; 198(11): 1677 - 1688.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
P. M. Baldini, P. De Vito, A. Martino, M. Fraziano, C. Grimaldi, P. Luly, F. Zalfa, and V. Colizzi
Differential sensitivity of human monocytes and macrophages to ANP: a role of intracellular pH on reactive oxygen species production through the phospholipase involvement
J. Leukoc. Biol., April 1, 2003; 73(4): 502 - 510.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. Giron-Calle, K. Srivatsa, and H. J. Forman
Priming of Alveolar Macrophage Respiratory Burst by H2O2 Is Prevented by Phosphatidylcholine-Specific Phospholipase C Inhibitor Tricyclodecan-9-yl-xanthate (D609)
J. Pharmacol. Exp. Ther., April 1, 2002; 301(1): 87 - 94.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2000 American Thoracic Society. 		  CCM abstracts