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Am. J. Respir. Cell Mol. Biol., Volume 24, Number 1, January, 2001 1-11

Triggering the Induction of Myofibroblast and Fibrogenesis by Airway Epithelial Shedding

Yuko Morishima, Akihiro Nomura, Yoshiyuki Uchida, Yoshiko Noguchi, Tohru Sakamoto, Yukio Ishii, Yukio Goto, Kuniko Masuyama, Min Jie Zhang, Kuniyoshi Hirano, Mie Mochizuki, Morio Ohtsuka, and Kiyohisa Sekizawa

Department of Pulmonary Medicine, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan

Myofibroblasts have been thought to participate in subepithelial fibrosis in asthma, but the mechanism of myofibroblast induction has not been fully understood. In this study we investigated injury-related myofibroblast induction in a coculture system of guinea-pig epithelial cells and fibroblasts cocultured in a human amnion chamber. After pseudostratified epithelial cells were mechanically scraped, migrated flat epithelial cells differentiated into cuboidal appearances on Day 4 and then returned to their original shapes on Day 8. During the course of the epithelial redifferentiation, it was found by Northern blot analysis, immunohistochemistry for alpha -smooth muscle actin, and electron microscopic observation that the myofibroblasts were transiently induced on Day 4. The myofibroblast induction was inhibited by the blocking of transforming growth factor (TGF)-beta 1 and thrombospondin (TSP)-1, indicating that the activation of TGF-beta 1 by TSP-1 would induce myofibroblasts. This finding was also supported by a transient upregulation of TSP immunoreactivity and TSP-1 messenger RNA (mRNA) in fibroblasts. Interestingly, epithelial injury reduced TGF-beta 1 immunoreactivity in the amnion membrane but did not affect TGF-beta 1 mRNA in epithelial cells and fibroblasts, indicating that TGF-beta 1 supplied from the extracellular matrix can participate in myofibroblast induction. Concurrently with myofibroblast induction, procollagen type I and III mRNAs were upregulated in fibroblasts, and obvious collagen deposition was observed ultrastructurally around the myofibroblasts compared with the fibroblasts. These results indicate that induced myofibroblasts can be functionally more active in producing collagen than are resting fibroblasts. The present study suggests that epithelial injury stimulates TGF-beta 1 release from the extracellular matrix and its activation via TSP-1 production, causing collagen synthesis through myofibroblast induction.




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