help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shannon, J. M.
Right arrow Articles by Mason, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shannon, J. M.
Right arrow Articles by Mason, R. J.

Am. J. Respir. Cell Mol. Biol., Volume 24, Number 3, March, 2001 235-244

Lung Fibroblasts Improve Differentiation of Rat Type II Cells in Primary Culture

John M. Shannon, Tianli Pan, Larry D. Nielsen, Karen E. Edeen, and Robert J. Mason

Department of Medicine, National Jewish Medical and Research Center; and Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Sciences Center, Denver, Colorado

Epithelial-mesenchymal interactions mediate prenatal lung morphogenesis and differentiation, yet little is known about their effects in the adult. In this study we have examined the influence of cocultured lung fibroblasts on rat alveolar type II cell differentiation in primary culture. Type II cells that were co-cultured with lung fibroblasts showed significant increases in messenger RNA (mRNA) levels of surfactant protein (SP)-A, SP-B, SP-C, and SP-D. Metabolic labeling and immunohistochemistry demonstrated that these mRNAs were translated and processed. Addition of 10-7 M dexamethasone (DEX) to cocultures antagonized the effects of the fibroblasts on SP-A and SP-C, but significantly augmented the effects on SP-B; expression of SP-D was unaffected. Coculture of type II cells with lung fibroblasts also increased acetate incorporation into phospholipids 10-fold, which was antagonized by DEX. Keratinocyte growth factor (KGF) mimicked the effects of lung fibroblasts on SP gene expression, but KGF neutralizing antibodies only partially reduced the effects of lung fibroblasts. KGF increased acetate incorporation into surfactant phospholipids, and the addition of DEX augmented this response. Together, our observations suggest that epithelial-mesenchymal interactions affect type II cell differentiation in the adult lung, and that these effects are partially mediated by KGF.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M.-L. Franco-Montoya, J. R. Bourbon, X. Durrmeyer, S. Lorotte, P.-H. Jarreau, and C. Delacourt
Pulmonary effects of keratinocyte growth factor in newborn rats exposed to hyperoxia
Am J Physiol Lung Cell Mol Physiol, November 1, 2009; 297(5): L965 - L976.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Dasgupta, R. Sakurai, Y. Wang, P. Guo, N. Ambalavanan, J. S. Torday, and V. K. Rehan
Hyperoxia-induced neonatal rat lung injury involves activation of TGF-{beta} and Wnt signaling and is protected by rosiglitazone
Am J Physiol Lung Cell Mol Physiol, June 1, 2009; 296(6): L1031 - L1041.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. R. Newman, E. Walsh, K. B. C. Apparao, and P. L. Sannes
Fibroblast growth factor-binding protein and N-deacetylase/N-sulfotransferase-1 expression in type II cells is modulated by heparin and extracellular matrix
Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1314 - L1320.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. J. Mondrinos, S. Koutzaki, P. I. Lelkes, and C. M. Finck
A tissue-engineered model of fetal distal lung tissue
Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L639 - L650.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Wang, K. Edeen, R. Manzer, Y. Chang, S. Wang, X. Chen, C.J. Funk, G. P. Cosgrove, X. Fang, and R. J. Mason
Differentiated Human Alveolar Epithelial Cells and Reversibility of their Phenotype In Vitro
Am. J. Respir. Cell Mol. Biol., June 1, 2007; 36(6): 661 - 668.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Deimling, K. Thompson, I. Tseu, J. Wang, R. Keijzer, A. K. Tanswell, and M. Post
Mesenchymal maintenance of distal epithelial cell phenotype during late fetal lung development
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L725 - L741.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. A. Leiner, D. Newman, C.-M. Li, E. Walsh, J. Khosla, and P. L. Sannes
Heparin and Fibroblast Growth Factors Affect Surfactant Protein Gene Expression in Type II Cells
Am. J. Respir. Cell Mol. Biol., November 1, 2006; 35(5): 611 - 618.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Chen, B. A. Hyatt, M. L. Mucenski, R. J. Mason, and J. M. Shannon
Identification and characterization of a lysophosphatidylcholine acyltransferase in alveolar type II cells
PNAS, August 1, 2006; 103(31): 11724 - 11729.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. Nishina, F. Zhang, L. D. Nielsen, K. Edeen, J. Wang, and R. J. Mason
Expression of CINC-2{beta} Is Related to the State of Differentiation of Alveolar Epithelial Cells
Am. J. Respir. Cell Mol. Biol., November 1, 2005; 33(5): 505 - 512.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. W. Lee, R. F. Gonzalez, C. J. Chapin, J. Busch, J. R. Fineman, and J. A. Gutierrez
Nitric oxide decreases surfactant protein gene expression in primary cultures of type II pneumocytes
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L950 - L957.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. Raoul, B. Chailley-Heu, A.-M. Barlier-Mur, C. Delacourt, B. Maitre, and J. R. Bourbon
Effects of vascular endothelial growth factor on isolated fetal alveolar type II cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1293 - L1301.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
F. Zhang, T. Pan, L. D. Nielsen, and R. J. Mason
Lipogenesis in Fetal Rat Lung: Importance of C/EBP{alpha}, SREBP-1c, and Stearoyl-CoA Desaturase
Am. J. Respir. Cell Mol. Biol., February 1, 2004; 30(2): 174 - 183.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A.-M. Barlier-Mur, B. Chailley-Heu, C. Pinteur, A. Henrion-Caude, C. Delacourt, and J. R. Bourbon
Maturational Factors Modulate Transcription Factors CCAAT/Enhancer-Binding Proteins {alpha}, {beta}, {delta}, and Peroxisome Proliferator-Activated Receptor-{gamma} in Fetal Rat Lung Epithelial Cells
Am. J. Respir. Cell Mol. Biol., November 1, 2003; 29(5): 620 - 626.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. E. Isakson, G. J. Seedorf, R. L. Lubman, W. H. Evans, and S. Boitano
Cell-Cell Communication in Heterocellular Cultures of Alveolar Epithelial Cells
Am. J. Respir. Cell Mol. Biol., November 1, 2003; 29(5): 552 - 561.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
F. Zhang, W. Pao, S. M. Umphress, S. B. Jakowlew, A. M. Meyer, L. D. Dwyer-Nield, L. D. Nielsen, K. Takeda, E. W. Gelfand, J. H. Fisher, et al.
Serum Levels of Surfactant Protein D Are Increased in Mice with Lung Tumors
Cancer Res., September 15, 2003; 63(18): 5889 - 5894.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. M. Roper, R. J. Staversky, J. N. Finkelstein, P. C. Keng, and M. A. O'Reilly
Identification and isolation of mouse type II cells on the basis of intrinsic expression of enhanced green fluorescent protein
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L691 - L700.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Fehrenbach, C. Bube, J. M. Hohlfeld, P. Stevens, T. Tschernig, H. G. Hoymann, N. Krug, and H. Fehrenbach
Surfactant Homeostasis Is Maintained In Vivo during Keratinocyte Growth Factor-induced Rat Lung Type II Cell Hyperplasia
Am. J. Respir. Crit. Care Med., May 1, 2003; 167(9): 1264 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Koval
Sharing signals: connecting lung epithelial cells with gap junction channels
Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L875 - L893.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. R. Rice, J. J. Conkright, C.-L. Na, M. Ikegami, J. M. Shannon, and T. E. Weaver
Maintenance of the mouse type II cell phenotype in vitro
Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L256 - L264.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. B. Ware and M. A. Matthay
Keratinocyte and hepatocyte growth factors in the lung: roles in lung development, inflammation, and repair
Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L924 - L940.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. R. Bates, L. W. Gonzales, J.-Q. Tao, P. Rueckert, P. L. Ballard, and A. B. Fisher
Recovery of rat type II cell surfactant components during primary cell culture
Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L267 - L276.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2001 American Thoracic Society. 		  New Orleans Int'l Conf