help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by White, S. R.
Right arrow Articles by Dorscheid, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by White, S. R.
Right arrow Articles by Dorscheid, D. R.

Am. J. Respir. Cell Mol. Biol., Volume 24, Number 3, March, 2001 282-294

Initiation of Apoptosis by Actin Cytoskeletal Derangement in Human Airway Epithelial Cells

Steven R. White, Paula Williams, Kimberly R. Wojcik, Steven Sun, Pieter S. Hiemstra, Klaus F. Rabe, and Delbert R. Dorscheid

Section of Pulmonary and Critical Care Medicine, Department of Medicine, Division of Biological Sciences, University of Chicago, Chicago, Illinois; and Department of Pulmonology, Leiden University Medical Center, Leiden, The Netherlands

Changes in epithelial cell shape can lead to cell death and detachment. Actin filaments are cleaved during apoptosis, but whether disruption in the actin cytoskeletal network, as one manifestation of cell shape change, can itself induce apoptosis is not known. We tested this hypothesis in the airway epithelial cell line 1HAEo- and in primary airway epithelial cells by preventing actin filament elongation with cytochalasin D or by aggregating actin filaments with jasplakinolide. Disruption of actin filament integrity promptly induced apoptosis in adherent epithelial cells within 5 h. Jasplakinolide-induced apoptosis did not disrupt focal adhesions, whereas cytochalasin D-induced apoptosis decreased focal adhesion protein expression and occurred despite ligation of the fibronectin receptor. Death induction was abrogated by the caspase inhibitors z-VAD-fmk and Ac-DEVD-cho but not by blocking the Fas (CD95) receptor. Whereas cytochalasin D-induced apoptosis was associated with cleavage of pro-caspase-8, jasplakinolide-induced apoptosis was not. Both agents induced formation of a death-inducing signaling complex. These data demonstrate that disruption of actin filament integrity with either cytochalasin D or jasplakinolide induces apoptosis in airway epithelial cells but by different mechanisms, and suggest that actin may be an early modulator of apoptotic commitment.

Abbreviations: N-acetyl-(Asp-Glu-Val)-3-amino-4-oxobutaonic acid, Ac-DEVD-cho; acetyl-Ile-Glu-Thr-Asp-alpha -7-amino-4-trifluoromethylcoumarin, Ac-IETD-AFC; acetyl-Ile-Glu-Thr-Asp-CHO aldehyde, Ac-IETD-CHO; bovine pituitary extract, BPE; death-inducing signaling complex, DISC; dimethylsulfoxide, DMSO; ethyleneglycol-bis-(beta -aminoethyl ether)-N,N'-tetraacetic acid, EGTA; fetal calf serum, FCS; glycine-arginine-glycine- aspartic acid-serine-proline, GRGDSP; glycine-arginine-glycine-glutamic acid-serine-proline, GRGESP; immunoglobulin, Ig; monoclonal antibody, mAb; Madin-Darby canine kidney, MDCK; normal human bronchial epithelial, NHBE; phosphate-buffered saline, PBS; sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE; terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling, TUNEL; Z-Val-Ala-Asp-fluoromethylketone, z-VAD-fmk.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
J. L. Vinas, G. Hotter, F. Pi, L. Palacios, and A. Sola
Role of peroxynitrite on cytoskeleton alterations and apoptosis in renal ischemia-reperfusion
Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1673 - F1680.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
F. Vandermoere, I. E. Yazidi-Belkoura, Y. Demont, C. Slomianny, J. Antol, J. Lemoine, and H. Hondermarck
Proteomics Exploration Reveals That Actin Is a Signaling Target of the Kinase Akt
Mol. Cell. Proteomics, January 1, 2007; 6(1): 114 - 124.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. R. Dorscheid, B. J. Patchell, O. Estrada, B. Marroquin, R. Tse, and S. R. White
Effects of corticosteroid-induced apoptosis on airway epithelial wound closure in vitro
Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L794 - L801.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Tesfaigzi
Roles of Apoptosis in Airway Epithelia
Am. J. Respir. Cell Mol. Biol., May 1, 2006; 34(5): 537 - 547.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. V. Cybulsky, T. Takano, J. Papillon, K. Bijian, and J. Guillemette
Activation of the extracellular signal-regulated kinase by complement C5b-9
Am J Physiol Renal Physiol, September 1, 2005; 289(3): F593 - F603.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Li, Q. Li, C. Xie, H. Zhou, Y. Wang, N. Zhang, H. Shao, S. C. Chan, X. Peng, S.-C. Lin, et al.
{beta}-actin is required for mitochondria clustering and ROS generation in TNF-induced, caspase-independent cell death
J. Cell Sci., September 15, 2004; 117(20): 4673 - 4680.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. S. Undevia, D. R. Dorscheid, B. A. Marroquin, W. L. Gugliotta, R. Tse, and S. R. White
Smad and p38-MAPK signaling mediates apoptotic effects of transforming growth factor-{beta}1 in human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L515 - L524.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Moore, B. A. Marroquin, W. Gugliotta, R. Tse, and S. R. White
Rho Kinase Inhibition Initiates Apoptosis in Human Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., March 1, 2004; 30(3): 379 - 387.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. V. Cybulsky, T. Takano, J. Papillon, A. Khadir, K. Bijian, and L. Le Berre
The actin cytoskeleton facilitates complement-mediated activation of cytosolic phospholipase A2
Am J Physiol Renal Physiol, March 1, 2004; 286(3): F466 - F476.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S.-J. Kim, S.-G. Hwang, I.-C. Kim, and J.-S. Chun
Actin Cytoskeletal Architecture Regulates Nitric Oxide-induced Apoptosis, Dedifferentiation, and Cyclooxygenase-2 Expression in Articular Chondrocytes via Mitogen-activated Protein Kinase and Protein Kinase C Pathways
J. Biol. Chem., October 24, 2003; 278(43): 42448 - 42456.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Hiruma, T. Katakura, S. Takahashi, T. Ichikawa, and T. Kawakami
Glutamate and Amyloid {beta}-Protein Rapidly Inhibit Fast Axonal Transport in Cultured Rat Hippocampal Neurons by Different Mechanisms
J. Neurosci., October 1, 2003; 23(26): 8967 - 8977.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. Tse, B. A. Marroquin, D. R. Dorscheid, and S. R. White
{beta}-Adrenergic agonists inhibit corticosteroid-induced apoptosis of airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L393 - L404.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
B. Jian, N. Narula, Q.-y. Li, E. R. Mohler III, and R. J. Levy
Progression of aortic valve stenosis: TGF-{beta}1 is present in calcified aortic valve cusps and promotes aortic valve interstitial cell calcification via apoptosis
Ann. Thorac. Surg., February 1, 2003; 75(2): 457 - 465.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
F. Bucchieri, S. M. Puddicombe, J. L. Lordan, A. Richter, D. Buchanan, S. J. Wilson, J. Ward, G. Zummo, P. H. Howarth, R. Djukanovic, et al.
Asthmatic Bronchial Epithelium Is More Susceptible to Oxidant-Induced Apoptosis
Am. J. Respir. Cell Mol. Biol., August 1, 2002; 27(2): 179 - 185.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Sanchez-Esteban, Y. Wang, L. A. Cicchiello, and L. P. Rubin
Pre- and Postnatal Lung Development, Maturation, and Plasticity: Cyclic mechanical stretch inhibits cell proliferation and induces apoptosis in fetal rat lung fibroblasts
Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L448 - L456.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. R. DORSCHEID, K. R. WOJCIK, S. SUN, B. MARROQUIN, and S. R. WHITE
Apoptosis of Airway Epithelial Cells Induced by Corticosteroids
Am. J. Respir. Crit. Care Med., November 15, 2001; 164(10): 1939 - 1947.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2001 American Thoracic Society.