help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wisnewski, A. V.
Right arrow Articles by Redlich, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wisnewski, A. V.
Right arrow Articles by Redlich, C. A.

Am. J. Respir. Cell Mol. Biol., Volume 24, Number 3, March, 2001 332-338

Human gamma /delta T-Cell Lines Derived from Airway Biopsies

Adam V. Wisnewski, Hilary Cain, Nadine Magoski, Hong Wang, Carole T. Holm, and Carrie A. Redlich

Pulmonary and Critical Care Section, Yale School of Medicine, New Haven, Connecticut

gamma /delta T cells have been postulated to play an important role in the immune response at epithelial boundaries, but have not been well described in human lung tissue. We have identified and characterized gamma /delta T-cell lines from human airway biopsies and compared them with T-cell lines from paired peripheral blood samples. Airway-derived T-cell lines stimulated with tetanus toxoid (TT) contained a greater proportion of gamma /delta T cells compared with T-cell lines stimulated with mitogens, other antigens, or without antigen. TT-stimulated airway T cells expressed different T-cell receptors (TCRs) than did blood- derived T cells, and used predominately variable region (V)gamma I family genes rather than Vgamma II family genes. Airway-derived gamma /delta T cells produced high levels of interferon-gamma and were associated with T helper 1-like cytokine profiles. This study describes the presence and antigen-dependent proliferation of gamma /delta T cells from human airway tissue, and demonstrates differences in lung-derived gamma /delta TCRs compared with gamma /delta T cells derived from peripheral blood. The data suggest that gamma /delta T cells may be functionally enriched in human airways relative to peripheral blood.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2001 American Thoracic Society. 		  ATS Coding and Billing Quarterly