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Am. J. Respir. Cell Mol. Biol., Volume 24, Number 6, June, 2001 727-732

Prevention of Polymerization of M and Z alpha 1-Antitrypsin (alpha 1-AT) with Trimethylamine N-Oxide
Implications for the Treatment of alpha 1-AT Deficiency

Glyn L. Devlin, Helen Parfrey, Deborah J. Tew, David A. Lomas, and Stephen P. Bottomley

Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia; and Respiratory Medicine Unit, Department of Medicine, University of Cambridge, Wellcome Trust Center for Molecular Mechanisms in Disease, Cambridge Institute for Medical Research, Cambridge, United Kingdom

alpha 1-Antitrypsin (alpha 1-AT) is the most abundant circulating proteinase inhibitor. The Z variant results in profound plasma deficiency as the mutant polymerizes within hepatocytes. The retained polymers are associated with cirrhosis, and the lack of circulating protein predisposes to early onset emphysema. We have investigated the role of the naturally occurring solute trimethylamine N-oxide (TMAO) in modulating the polymerization of normal M and disease-associated Z alpha 1-AT. TMAO stabilized both M and Z alpha 1-AT in an active conformation against heat-induced polymerization. Spectroscopic analysis demonstrated that this was due to inhibition of the conversion of the native state to a polymerogenic intermediate. However, TMAO did not aid the refolding of denatured alpha 1-AT to a native conformation; instead, it enhanced polymerization. These data show that TMAO can be used to control the conformational transitions of folded alpha 1-AT but that it is ineffective in promoting folding of the polypeptide chain within the secretory pathway.

Abbreviations: alpha 1-antitrypsin, alpha 1-AT; 4,4'-di-anilino-1,1'-binaphthyl-5,5'-disulfonate, bis-ANS; circular dichroism, CD; guanidine hydrochloride, GuHCl; association rate constant, kass; emission wavelength, lambda em; excitation wavelength, lambda ex; polyacrylamide gel electrophoresis, PAGE; stoichiometry of inhibition, SI; melting temperature, TM; trimethylamine N-oxide, TMAO.




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