help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujimoto, K.
Right arrow Articles by Satoh, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujimoto, K.
Right arrow Articles by Satoh, K.

Am. J. Respir. Cell Mol. Biol., Volume 25, Number 2, August, 2001 233-238

Interferon-gamma Stimulates Fractalkine Expression in Human Bronchial Epithelial Cells and Regulates Mononuclear Cell Adherence

Koji Fujimoto, Tadaatsu Imaizumi, Hidemi Yoshida, Shingo Takanashi, Ken Okumura, and Kei Satoh

Department of Vascular Biology, Institute of Brain Science; and The Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan

Bronchial epithelial cells may contribute to airway inflammation by releasing chemokines and expressing surface membrane molecules involved in the adhesion of leukocytes. We found that interferon (IFN)-gamma stimulates expression of fractalkine, a potent chemoattractant for monocytes and T lymphocytes, in a time- and concentration-dependent manner by normal human bronchial epithelial cells in culture. Enhanced expression of fractalkine messenger RNA was confirmed by both reverse transcription/polymerase chain reaction and Northern blotting. IFN-gamma also stimulated fractalkine protein production and most of the protein was found in cell lysates. The adherence of blood mononuclear cells to the monolayers of bronchial epithelial cells stimulated with IFN-gamma was partly inhibited by an antifractalkine antibody. An antibody against intercellular adhesion molecule-1 was similarly effective in inhibiting the adhesion. Fractalkine protein levels in bronchoalveolar lavage fluids from patients with inflammatory diseases correlated positively with mononuclear cell counts in the fluids. The bronchial epithelium in a biopsy specimen of lung cancer was stained positively by immunofluorescent staining for fractalkine. We conclude that IFN-gamma stimulates fractalkine expression by bronchial epithelial cells, which may play an important role in inflammatory responses by recruiting mononuclear leukocytes to the bronchial epithelium.

Abbreviations: bronchoalveolar lavage fluid, BALF; digoxygenin, DIG; enzyme-linked immunosorbent assay, ELISA; glyceraldehyde-3-phosphate dehydrogenase, GAPDH, intercellular adhesion molecule, ICAM; interferon, IFN; immunoglobulin, Ig; monocyte chemotactic protein, MCP; messenger RNA, mRNA; polymerase chain reaction, PCR; polymorphonuclear neutrophil, PMN; reverse transcriptase, RT.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Pachot, M.-A. Cazalis, F. Venet, F. Turrel, C. Faudot, N. Voirin, J. Diasparra, N. Bourgoin, F. Poitevin, B. Mougin, et al.
Decreased Expression of the Fractalkine Receptor CX3CR1 on Circulating Monocytes as New Feature of Sepsis-Induced Immunosuppression
J. Immunol., May 1, 2008; 180(9): 6421 - 6429.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Mizutani, T. Sakurai, T. Shibata, K. Uchida, J. Fujita, R. Kawashima, Y. I. Kawamura, N. Toyama-Sorimachi, T. Imai, and T. Dohi
Dose-Dependent Differential Regulation of Cytokine Secretion from Macrophages by Fractalkine
J. Immunol., December 1, 2007; 179(11): 7478 - 7487.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Harcourt, R. Alvarez, L. P. Jones, C. Henderson, L. J. Anderson, and R. A. Tripp
Respiratory Syncytial Virus G Protein and G Protein CX3C Motif Adversely Affect CX3CR1+ T Cell Responses
J. Immunol., February 1, 2006; 176(3): 1600 - 1608.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Suzuki, T. Nanki, T. Imai, H. Kikuchi, S. Hirohata, H. Kohsaka, and N. Miyasaka
Inhibition of CX3CL1 (Fractalkine) Improves Experimental Autoimmune Myositis in SJL/J Mice
J. Immunol., November 15, 2005; 175(10): 6987 - 6996.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
T. Imaizumi, M. Kumagai, K. Taima, T. Fujita, H. Yoshida, and K. Satoh
Involvement of retinoic acid-inducible gene-I in the IFN-{gamma}/STAT1 signalling pathway in BEAS-2B cells
Eur. Respir. J., June 1, 2005; 25(6): 1077 - 1083.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
P. J. Barnes
Mediators of Chronic Obstructive Pulmonary Disease
Pharmacol. Rev., December 1, 2004; 56(4): 515 - 548.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. B. Sukkar, R. Issa, S. Xie, U. Oltmanns, R. Newton, and K. F. Chung
Fractalkine/CX3CL1 production by human airway smooth muscle cells: induction by IFN-{gamma} and TNF-{alpha} and regulation by TGF-{beta} and corticosteroids
Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1230 - L1240.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Yamamoto, I. Kobayashi, K. Tsuji, N. Nishi, E. Muro, M. Miyazaki, M. Zaitsu, S. Inada, T. Ichimaru, and Y. Hamasaki
Upregulation of Interleukin-4 Receptor by Interferon-{gamma}: Enhanced Interleukin-4-Induced Eotaxin-3 Production in Airway Epithelium
Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 456 - 462.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
O. Zegarra-Moran, C. Folli, B. Manzari, R. Ravazzolo, L. Varesio, and L. J. V. Galietta
Double Mechanism for Apical Tryptophan Depletion in Polarized Human Bronchial Epithelium
J. Immunol., July 1, 2004; 173(1): 542 - 549.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
L. R. Latchney, M. A. Fallon, D. J. Culp, H. A. Gelbard, and S. Dewhurst
Immunohistochemical Assessment of Fractalkine, Inflammatory Cells, and Human Herpesvirus 7 in Human Salivary Glands
J. Histochem. Cytochem., May 1, 2004; 52(5): 671 - 682.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
H. Umehara, E. T. Bloom, T. Okazaki, Y. Nagano, O. Yoshie, and T. Imai
Fractalkine in Vascular Biology: From Basic Research to Clinical Disease
Arterioscler. Thromb. Vasc. Biol., January 1, 2004; 24(1): 34 - 40.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
E. Lavergne, B. Combadiere, O. Bonduelle, M. Iga, J.-L. Gao, M. Maho, A. Boissonnas, P. M. Murphy, P. Debre, and C. Combadiere
Fractalkine Mediates Natural Killer-Dependent Antitumor Responses in Vivo
Cancer Res., November 1, 2003; 63(21): 7468 - 7474.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. M. Haynes, L. P. Jones, A. Barskey, L. J. Anderson, and R. A. Tripp
Enhanced Disease and Pulmonary Eosinophilia Associated with Formalin-Inactivated Respiratory Syncytial Virus Vaccination Are Linked to G Glycoprotein CX3C-CX3CR1 Interaction and Expression of Substance P
J. Virol., September 15, 2003; 77(18): 9831 - 9844.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2001 American Thoracic Society.