help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Horvath, G.
Right arrow Articles by Wanner, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Horvath, G.
Right arrow Articles by Wanner, A.

Am. J. Respir. Cell Mol. Biol., Volume 25, Number 4, October, 2001 500-506

Steroid Sensitivity of Norepinephrine Uptake by Human Bronchial Arterial and Rabbit Aortic Smooth Muscle Cells

Gabor Horvath, Thomas Lieb, Gregory E. Conner, Matthias Salathe, and Adam Wanner

Division of Pulmonary and Critical Care Medicine, and Department of Cell Biology and Anatomy, University of Miami School of Medicine, Miami, Florida

We have shown that an inhaled glucocorticosteroid (GS) causes alpha 1-adrenergic antagonist-blockable, rapid, and transient bronchial vasoconstriction in healthy and asthmatic subjects. Steroids inhibit norepinephrine (NE) uptake by non-neuronal cells, thereby increasing NE concentration at alpha -adrenergic receptor sites. This could explain the GS-induced bronchial vasoconstriction. We therefore studied expression of the steroid-sensitive extraneuronal monoamine transporter (EMT) and steroid sensitivity of NE uptake in human bronchial artery and rabbit aorta (as a substitute for the limited supply of human bronchial artery). NE uptake was measured using a semiquantitative, sucrose-potassium phosphate-glyoxylic acid fluorescence method that we newly adapted for use in single cells. Both human bronchial arteries and rabbit aorta expressed messenger RNA for EMT, and steroids blocked NE uptake into freshly dissociated human bronchial arterial and rabbit aortic smooth-muscle cells (SMCs). In the latter, inhibition of NE uptake by steroids was not altered, either by a protein synthesis inhibitor (cycloheximide) or by a transcription inhibitor (actinomycin D), and corticosterone made membrane-impermeant by conjugation to bovine serum albumin inhibited NE uptake equipotently. These data show that NE uptake into bronchial arterial and rabbit aortic SMCs is sensitive to steroids, possibly mediated by EMT, and suggest a mechanism for GS-induced bronchial vasoconstriction.

Abbreviations: complementary DNA, cDNA; catecholamine-O-methyltransferase, COMT; differential-interference-contrast, DIC; extraneuronal monoamine transporter, EMT; mean fluorescence intensity value, FN; glucocorticosteroid, GS; Michaelis constant, Km; monoamine oxidase, MAO; messenger RNA, mRNA; norepinephrine, NE; organic cation transporter, OCT; reverse transcriptase/polymerase chain reaction, RT-PCR; standard error of the mean, SEM; smooth muscle cell, SMC; sucrose- potassium phosphate-glyoxylic acid, SPG.




This article has been cited by other articles:


Home page
 ThoraxHome page
P Paredi and P J Barnes
The airway vasculature: recent advances and clinical implications
Thorax, May 1, 2009; 64(5): 444 - 450.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
E. Livingston, R. Chaudhuri, A. D. McMahon, I. Fraser, C. P. McSharry, and N. C. Thomson
Systemic sensitivity to corticosteroids in smokers with asthma
Eur. Respir. J., January 1, 2007; 29(1): 64 - 71.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Horvath, N. Schmid, M. A. Fragoso, A. Schmid, G. E. Conner, M. Salathe, and A. Wanner
Epithelial Organic Cation Transporters Ensure pH-Dependent Drug Absorption in the Airway
Am. J. Respir. Cell Mol. Biol., January 1, 2007; 36(1): 53 - 60.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. Chaudhuri, E. Livingston, A. D. McMahon, J. Lafferty, I. Fraser, M. Spears, C. P. McSharry, and N. C. Thomson
Effects of Smoking Cessation on Lung Function and Airway Inflammation in Smokers with Asthma
Am. J. Respir. Crit. Care Med., July 15, 2006; 174(2): 127 - 133.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
G. Horvath and A. Wanner
Inhaled corticosteroids: effects on the airway vasculature in bronchial asthma
Eur. Respir. J., January 1, 2006; 27(1): 172 - 187.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. Horvath, Z. Sutto, A. Torbati, G. E. Conner, M. Salathe, and A. Wanner
Norepinephrine transport by the extraneuronal monoamine transporter in human bronchial arterial smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L829 - L837.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
E.S. Mendes, A. Pereira, I. Danta, R.C. Duncan, and A. Wanner
Comparative bronchial vasoconstrictive efficacy of inhaled glucocorticosteroids
Eur. Respir. J., June 1, 2003; 21(6): 989 - 993.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Horvath, A. Torbati, G. E. Conner, M. Salathe, and A. Wanner
Systemic Ovalbumin Sensitization Downregulates Norepinephrine Uptake by Rabbit Aortic Smooth Muscle Cells
Am. J. Respir. Cell Mol. Biol., December 1, 2002; 27(6): 746 - 751.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A.A. Acuna, J. Gabrijelcic, E.M. Uribe, R. Rabinovich, J. Roca, J.A. Barbera, K.F. Chung, and R. Rodriguez-Roisin
Fluticasone propionate attenuates platelet-activating factor-induced gas exchange defects in mild asthma
Eur. Respir. J., May 1, 2002; 19(5): 872 - 878.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2001 American Thoracic Society. 		  ATS Coding and Billing Quarterly