help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Million, K.
Right arrow Articles by Marano, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Million, K.
Right arrow Articles by Marano, F.

Am. J. Respir. Cell Mol. Biol., Volume 25, Number 6, December, 2001 744-750

Effects of Retinoic Acid Receptor-Selective Agonists on Human Nasal Epithelial Cell Differentiation

Karine Million, Frédéric Tournier, Odile Houcine, Philippe Ancian, Uwe Reichert, and Francelyne Marano

Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Paris; and Galderma, Research and Development, Valbonne, France

Retinoids play a critical role in the maintenance of the mucociliary phenotype of epithelial cells in the upper respiratory tract. To determine the role of retinoic acid receptors (RARs) in the regulation of epithelial differentiation, we tested the effect of the synthetic retinoids CD336, CD2019, and CD666, selective agonists for RARalpha , RARbeta , and RARgamma , respectively, during differentiation of human nasal epithelial (HNE) cells in vitro. Using glutamylated tubulin and transglutaminase I (Tg I) as markers of ciliated cell and squamous cell differentiation, respectively, we showed that retinoic acid (RA) stimulated mucociliary differentiation and, in parallel, inhibited squamous cell differentiation. The agonists of the three RARs independently induced ciliogenesis and inhibited squamous cell differentiation by downregulating Tg I expression in a dose- and time-dependent manner. Antagonists specific for the three RARs abolished the effects of the corresponding agonists, demonstrating an RAR-specific mediated effect. Moreover, treatment of retinoid-deficient cultures with RAR agonists induced conversion of the squamous-like phenotype into a ciliated phenotype. In conclusion, all three RARs are potentially involved in the differentiating effects of RA in respiratory epithelial cells.

Abbreviations: air-liquid interface, ALI; bovine serum albumin, BSA; Dulbecco's modified Eagle medium, DMEM; fluorescein isothiocyanate, FITC; forward angle scatter, FSC; human nasal epithelial, HNE; phosphate-buffered saline, PBS; retinoic acid, RA; retinoic acid receptor, RAR; retinoic acid response element, RARE; reverse transcriptase/polymerase chain reaction, RT-PCR; retinoid X receptor, RXR; right angle scatter, SSC; Tris-buffered saline, TBS; transglutaminase I, Tg I.




This article has been cited by other articles:


Home page
 J. Med. Genet.Home page
M Geremek, E Zietkiewicz, S R Diehl, B Z Alizadeh, C Wijmenga, and M Witt
Linkage analysis localises a Kartagener syndrome gene to a 3.5 cM region on chromosome 15q24-25
J. Med. Genet., January 1, 2006; 43(1): e1 - e1.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2001 American Thoracic Society.