help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sekhon, H. S.
Right arrow Articles by Spindel, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sekhon, H. S.
Right arrow Articles by Spindel, E. R.

Am. J. Respir. Cell Mol. Biol., Volume 26, Number 1, January, 2002 31-41

Maternal Nicotine Exposure Upregulates Collagen Gene Expression in Fetal Monkey Lung
Association with alpha 7 Nicotinic Acetylcholine Receptors

Harmanjatinder S. Sekhon, Jennifer A. Keller, Becky J. Proskocil, Ellen L. Martin, and Eliot R. Spindel

Division of Neuroscience, Oregon Regional Primate Research Center, Beaverton, Oregon; and Department of Pathology, Oregon Health Sciences University, Portland, Oregon

The recent identification of nicotinic acetylcholine receptors (nAChR) in pulmonary fibroblasts suggests that in utero nicotine exposure may alter collagen expression by these cells in the developing lung. To test this hypothesis, timed-pregnant rhesus monkeys were administered nicotine (1-1.5 mg/kg/d, subcutaneously) using osmotic minipumps from Days 26-134 or 26-160 of gestation (term = 165 d). In utero nicotine exposure significantly increased airway wall area per unit epithelial basement membrane. Collagen type I and III mRNA expression and immunostaining were significantly increased in the airway and alveolar walls of the nicotine-treated group. Elastin mRNA expression increased, but protein expression in parenchyma remained unchanged. Dual labeling studies colocalized alpha 7 nAChR and collagen to the same cells in airway wall cells, and colocalization of alpha 7 nAChR and collagen was confirmed in isolated pulmonary fibroblasts. These findings suggest that nicotine may directly interact with alpha 7 nAChR to increase collagen accumulation in airway and alveolar walls following in utero nicotine exposure. These data suggest that passage of nicotine across the placenta to increase collagen deposition and therefore increase airway wall dimensions in fetal lung may partially explain the observed alterations in lung mechanics in the infants of mothers who smoke during pregnancy.


Abbreviations: critical threshold, CT; epithelial basement membrane, EpiBM; nicotinic cholinergic receptors, nAChR; phosphate-buffered saline, PBS; ratio of time to peak tidal expiratory flow to total expiratory time, TPTEF:TE; tris-buffered saline, TBS.




This article has been cited by other articles:


Home page
Am. J. Respir. Cell Mol. Bio.Home page
X. W. Fu, J. Lindstrom, and E. R. Spindel
Nicotine Activates and Up-Regulates Nicotinic Acetylcholine Receptors in Bronchial Epithelial Cells
Am. J. Respir. Cell Mol. Biol., July 1, 2009; 41(1): 93 - 99.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
M. J. Blacquiere, W. Timens, B. N. Melgert, M. Geerlings, D. S. Postma, and M. N. Hylkema
Maternal smoking during pregnancy induces airway remodelling in mice offspring
Eur. Respir. J., May 1, 2009; 33(5): 1133 - 1140.
[Abstract] [Full Text] [PDF]


Home page
Br. J. Sports. Med.Home page
P Danielson
Reviving the "biochemical" hypothesis for tendinopathy: new findings suggest the involvement of locally produced signal substances
Br. J. Sports Med., April 1, 2009; 43(4): 265 - 268.
[Full Text] [PDF]


Home page
Br. J. Sports. Med.Home page
E Zeisig, B-O Ljung, H Alfredson, and P Danielson
Immunohistochemical evidence of local production of catecholamines in cells of the muscle origins at the lateral and medial humeral epicondyles: of importance for the development of tennis and golfer's elbow?
Br. J. Sports Med., April 1, 2009; 43(4): 269 - 275.
[Abstract] [Full Text] [PDF]


Home page
HeartHome page
A. Goette, U. Lendeckel, A. Kuchenbecker, A. Bukowska, B. Peters, H. U Klein, C. Huth, and C. Rocken
Cigarette smoking induces atrial fibrosis in humans via nicotine
Heart, September 1, 2007; 93(9): 1056 - 1063.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Wongtrakool, S. Roser-Page, H. N. Rivera, and J. Roman
Nicotine alters lung branching morphogenesis through the {alpha}7 nicotinic acetylcholine receptor
Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L611 - L618.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. K. Rehan, Y. Wang, S. Sugano, J. Santos, S. Patel, R. Sakurai, L. W. Boros, W.-P. Lee, and J. S. Torday
In utero nicotine exposure alters fetal rat lung alveolar type II cell proliferation, differentiation, and metabolism
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L323 - L333.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Cell Mol. Bio.Home page
S. Matthiesen, A. Bahulayan, S. Kempkens, S. Haag, M. Fuhrmann, C. Stichnote, U. R. Juergens, and K. Racke
Muscarinic Receptors Mediate Stimulation of Human Lung Fibroblast Proliferation
Am. J. Respir. Cell Mol. Biol., December 1, 2006; 35(6): 621 - 627.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. K. Rehan, Y. Wang, S. Sugano, S. Romero, X. Chen, J. Santos, A. Khazanchi, and J. S. Torday
Mechanism of nicotine-induced pulmonary fibroblast transdifferentiation
Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L667 - L676.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
M. Miyauchi, Z. Qu, Y. Miyauchi, S.-M. Zhou, H. Pak, W. J. Mandel, M. C. Fishbein, P.-S. Chen, and H. S. Karagueuzian
Chronic nicotine in hearts with healed ventricular myocardial infarction promotes atrial flutter that resembles typical human atrial flutter
Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2878 - H2886.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
B. J. Proskocil, H. S. Sekhon, J. A. Clark, S. L. Lupo, Y. Jia, W. M. Hull, J. A. Whitsett, B. C. Starcher, and E. R. Spindel
Vitamin C Prevents the Effects of Prenatal Nicotine on Pulmonary Function in Newborn Monkeys
Am. J. Respir. Crit. Care Med., May 1, 2005; 171(9): 1032 - 1039.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
R. S. Tepper, T. Williams-Nkomo, T. Martinez, J. Kisling, C. Coates, and J. Daggy
Parental Smoking and Airway Reactivity in Healthy Infants
Am. J. Respir. Crit. Care Med., January 1, 2005; 171(1): 78 - 82.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
H.S. Sekhon, B.J. Proskocil, J.A. Clark, and E.R. Spindel
Prenatal nicotine exposure increases connective tissue expression in foetal monkey pulmonary vessels
Eur. Respir. J., June 1, 2004; 23(6): 906 - 915.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
B. J. Proskocil, H. S. Sekhon, Y. Jia, V. Savchenko, R. D. Blakely, J. Lindstrom, and E. R. Spindel
Acetylcholine Is an Autocrine or Paracrine Hormone Synthesized and Secreted by Airway Bronchial Epithelial Cells
Endocrinology, May 1, 2004; 145(5): 2498 - 2506.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
X. Q. Xiao, K. L. Grove, B. E. Grayson, and M. S. Smith
Inhibition of Uncoupling Protein Expression during Lactation: Role of Leptin
Endocrinology, February 1, 2004; 145(2): 830 - 838.
[Abstract] [Full Text] [PDF]


Home page
J. Histochem. Cytochem.Home page
K. S. Lips, U. Pfeil, K. Reiners, C. Rimasch, K. Kuchelmeister, R. C. Braun-Dullaeus, R. V. Haberberger, R. Schmidt, and W. Kummer
Expression of the High-affinity Choline Transporter CHT1 in Rat and Human Arteries
J. Histochem. Cytochem., December 1, 2003; 51(12): 1645 - 1654.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
P. Song, H. S. Sekhon, Y. Jia, J. A. Keller, J. K. Blusztajn, G. P. Mark, and E. R. Spindel
Acetylcholine Is Synthesized by and Acts as an Autocrine Growth Factor for Small Cell Lung Carcinoma
Cancer Res., January 1, 2003; 63(1): 214 - 221.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Cell Mol. Bio.Home page
R. A. Pierce and N. M. Nguyen
Prenatal Nicotine Exposure and Abnormal Lung Function
Am. J. Respir. Cell Mol. Biol., January 1, 2002; 26(1): 10 - 13.
[Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2002 American Thoracic Society.