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Am. J. Respir. Cell Mol. Biol., Volume 26, Number 2, February, 2002 183-188

Transforming Growth Factor-beta Stabilizes Elastin mRNA by a Pathway Requiring Active Smads, Protein Kinase C-delta , and p38

Umberto Kucich, Joan C. Rosenbloom, William R. Abrams, and Joel Rosenbloom

Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pennsylvania

Transforming growth factors (TGFs)-beta are multipotent in their biologic activity, regulating cell growth and differentiation as well as extracellular matrix deposition and degradation. Most of these activities involve modulation of gene transcription, but TGF-beta 1 has been shown previously to substantially increase the expression of elastin by stabilization of tropoelastin mRNA through a signaling pathway that likely involves a phosphatidylcholine-specific phospholipase C, a protein kinase C, prenylated and acylated protein(s), and one or more tyrosine kinases. However, there is a 4- to 6-h lag period after the addition of TGF-beta 1 before significant stimulation of elastin expression is observed and the question of whether the Smads are involved has not been addressed. In the present work, using cultured human fetal lung fibroblasts, we show through the use of specific inhibitors and transfection of a Smad 7 construct that in addition to de novo protein synthesis and active Smads, the extended activity of protein kinase C (PKC)-delta and the stress-activated protein kinase, p38, is required for TGF-beta 1 to achieve elastin mRNA stabilization.

Abbreviations: Dulbecco's modified Eagle's medium, DMEM; fluorescence-activated cell sorting, FACS; glyceraldehyde phosphate dehydrogenase, GAPDH; latent TGF-beta -binding protein, LTBP; mitogen-activated protein kinase, MAPK; phosphatidylcholine-specific phospholipase C, PC-PLC; protein kinase C, PKC; stress-activated protein kinase, SAPK; TGF-beta -activated kinase, TAK1; transforming growth factor, TGF.




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