help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carayol, N.
Right arrow Articles by Chanez, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carayol, N.
Right arrow Articles by Chanez, P.

Am. J. Respir. Cell Mol. Biol., Volume 26, Number 3, March, 2002 341-347

Modulation of Cadherin and Catenins Expression by Tumor Necrosis Factor-alpha and Dexamethasone in Human Bronchial Epithelial Cells

Nathalie Carayol, Alison Campbell, Isabelle Vachier, Brigitte Mainprice, Jean Bousquet, Philippe Godard, and Pascal Chanez

Clinique des Maladies Respiratoires, Hôpital Arnaud de Villeneuve, Montpellier, France

Asthma is an inflammatory disease, and the epithelial mesenchymal unit appears to be of importance in regulating the disease mechanisms. Cell-cell adhesion plays an important role in tissue morphogenesis and homeostasis and is commonly mediated by cadherins, a family of Ca2+-dependent transmembrane adhesion receptors. The cadherin family is involved in control of the cellular architecture. Proinflammatory cytokines such as tumor necrosis factor (TNF)-alpha are involved in asthma and may interfere with epithelial integrity. In the present study, we investigated the role of TNF-alpha and dexamethasone on the expression of E-cadherin, beta -catenin, and gamma -catenin. We used two bronchial epithelial cell models: primary small airway epithelial cell cultures and primary culture obtained from human bronchial tubes. After 48 h of TNF-alpha stimulation with or without dexamethasone expression of E-cadherin, beta -catenin and gamma -catenin were analyzed using Western blot analysis and immunofluorescence. This study showed a decrease in the expression of adhesion molecules in both epithelial cell cultures after stimulation. Dexamethasone and anti-TNF-alpha inhibited this effect. In unstimulated cells, E-cadherin and beta - and gamma -catenin expression was membranous, expressed only on the lateral cell wall with minimal cytoplasmic expression. Immunoreactivity was cytoplasmic in stimulated cells. We demonstrated, using Western blot analysis and immunofluorescence, that proinflammatory cytokines could be responsible for structural damage to the epithelium and that this process was potentially reversed by steroids.

Abbreviations: bovine serum albumin, BSA; ethylenediaminetetraacetic acid, EDTA; human bronchial epithelial cells, HBEC; human umbilical vein cells, HUVEC; interferon, IFN; interleukin, IL; nuclear factor, NF; phosphate-buffered saline, PBS; small airway epithelial cell, SAEC; tumor necrosis factor, TNF.




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
P. Panagopoulou, C. H. Davos, D. J. Milner, E. Varela, J. Cameron, D. L. Mann, and Y. Capetanaki
Desmin mediates TNF-{alpha}-induced aggregate formation and intercalated disk reorganization in heart failure
J. Cell Biol., May 28, 2008; 181(5): 761 - 775.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Dagenais, R. Frechette, M.-E. Clermont, C. Masse, A. Prive, E. Brochiero, and Y. Berthiaume
Dexamethasone inhibits the action of TNF on ENaC expression and activity
Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1220 - L1231.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Kim, A. J. Schein, and J. A. Nadel
E-cadherin promotes EGFR-mediated cell differentiation and MUC5AC mucin expression in cultured human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1049 - L1060.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. M. Kilani, K. A. Mohammed, N. Nasreen, J. A. Hardwick, M. H. Kaplan, R. S. Tepper, and V. B. Antony
Respiratory Syncytial Virus Causes Increased Bronchial Epithelial Permeability
Chest, July 1, 2004; 126(1): 186 - 191.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
A Bourdin, I Serre, H Flamme, P Vic, D Neveu, P Aubas, P Godard, and P Chanez
Can endobronchial biopsy analysis be recommended to discriminate between asthma and COPD in routine practice?
Thorax, June 1, 2004; 59(6): 488 - 493.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. T. Nguyen, J. Arredondo, A. I. Chernyavsky, Y. Kitajima, M. Pittelkow, and S. A. Grando
Pemphigus Vulgaris IgG and Methylprednisolone Exhibit Reciprocal Effects on Keratinocytes
J. Biol. Chem., January 16, 2004; 279(3): 2135 - 2146.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. Lyu, J.-A Kim, S. K. Chung, K.-S. Kim, and C.-K. Joo
Alteration of Cadherin in Dexamethasone-Induced Cataract Organ-Cultured Rat Lens
Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 2034 - 2040.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
N. Carayol, I. Vachier, A. Campbell, L. Crampette, J. Bousquet, P. Godard, and P. Chanez
Regulation of E-cadherin expression by dexamethasone and tumour necrosis factor-{alpha} in nasal epithelium
Eur. Respir. J., December 1, 2002; 20(6): 1430 - 1436.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2002 American Thoracic Society.