Am. J. Respir. Cell Mol. Biol., Volume 26, Number 5, May, 2002 541-548

Expression Profiling of the Developing Mouse Lung
Insights into the Establishment of the Extracellular Matrix

Thomas J. Mariani, Jeremy J. Reed, and Steven D. Shapiro

Departments of Pediatrics, Cell Biology and Physiology, Internal Medicine, and the Program in Lung Development, Washington University School of Medicine and Saint Louis Children's Hospital, St. Louis, Missouri

We have undertaken a comprehensive gene expression profiling of the entire process of murine lung development using oligonucleotide-based microarrays. Our data reveals the expression pattern of ~ 11,000 genes throughout the morphologic stages of lung development. This includes known genes with unappreciated pulmonary expression and novel genes with undefined functions. Traditional gene expression analysis techniques verify a high degree of confidence in the microarray data. Examination of the data confirms previously known patterns of expression for extracellular matrix genes and provides new information regarding relationships in temporal expression among groups of these genes. Large-scale cluster analysis reveals associations in the expression profile of specific genes with defined developmental processes. For instance, we identify groups of genes, which are coordinately expressed with extracellular matrix genes during lung development. These data should serve as a resource for the pulmonary research community and assist in deciphering the molecular mechanisms governing normal lung development as well as those involved in aberrant developmental pathology.

This article has been cited by other articles:

				S. E. Calvo, D. J. Pagliarini, and V. K. Mootha Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans PNAS, May 5, 2009; 106(18): 7507 - 7512. [Abstract] [Full Text] [PDF]

				S. Bhattacharya, S. Srisuma, D. L. DeMeo, S. D. Shapiro, R. Bueno, E. K. Silverman, J. J. Reilly, and T. J. Mariani Molecular Biomarkers for Quantitative and Discrete COPD Phenotypes Am. J. Respir. Cell Mol. Biol., March 1, 2009; 40(3): 359 - 367. [Abstract] [Full Text] [PDF]

				C. Pritchard, B. Mecham, R. Dumpit, I. Coleman, M. Bhattacharjee, Q. Chen, R. A. Sikes, and P. S. Nelson Conserved Gene Expression Programs Integrate Mammalian Prostate Development and Tumorigenesis Cancer Res., March 1, 2009; 69(5): 1739 - 1747. [Abstract] [Full Text] [PDF]

				A. T. Kho, S. Bhattacharya, B. H. Mecham, J. Hong, I. S. Kohane, and T. J. Mariani Expression Profiles of the Mouse Lung Identify a Molecular Signature of Time-to-Birth Am. J. Respir. Cell Mol. Biol., January 1, 2009; 40(1): 47 - 57. [Abstract] [Full Text] [PDF]

				K. Huang, R. Rabold, E. Abston, B. Schofield, V. Misra, E. Galdzicka, H. Lee, S. Biswal, W. Mitzner, and C. G. Tankersley Effects of leptin deficiency on postnatal lung development in mice J Appl Physiol, July 1, 2008; 105(1): 249 - 259. [Abstract] [Full Text] [PDF]

				C. P. Hersh, D. L. DeMeo, and E. K. Silverman National Emphysema Treatment Trial State of the Art: Genetics of Emphysema Proceedings of the ATS, May 1, 2008; 5(4): 486 - 493. [Abstract] [Full Text] [PDF]

				O. Boucherat, M.-L. Franco-Montoya, C. Thibault, R. Incitti, B. Chailley-Heu, C. Delacourt, and J. R. Bourbon Gene expression profiling in lung fibroblasts reveals new players in alveolarization Physiol Genomics, December 19, 2007; 32(1): 128 - 141. [Abstract] [Full Text] [PDF]

				V. Misra, H. Lee, A. Singh, K. Huang, R. K. Thimmulappa, W. Mitzner, S. Biswal, and C. G. Tankersley Global expression profiles from C57BL/6J and DBA/2J mouse lungs to determine aging-related genes Physiol Genomics, November 14, 2007; 31(3): 429 - 440. [Abstract] [Full Text] [PDF]

				H. Inaoka, Y. Fukuoka, and I. S. Kohane Evidence of spatially bound gene regulation in Mus musculus: Decreased gene expression proximal to microRNA genomic location PNAS, March 20, 2007; 104(12): 5020 - 5025. [Abstract] [Full Text] [PDF]

				T. Weng, Z. Chen, N. Jin, L. Gao, and L. Liu Gene expression profiling identifies regulatory pathways involved in the late stage of rat fetal lung development Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L1027 - L1037. [Abstract] [Full Text] [PDF]

				I. Mandeville, J. Aubin, M. LeBlanc, M. Lalancette-Hebert, M.-F. Janelle, G. M. Tremblay, and L. Jeannotte Impact of the Loss of Hoxa5 Function on Lung Alveogenesis Am. J. Pathol., October 1, 2006; 169(4): 1312 - 1327. [Abstract] [Full Text] [PDF]

				R. A. Pierce, C. H. Moore, and M. C. Arikan Positive transcriptional regulatory element located within exon 1 of elastin gene. Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L391 - L399. [Abstract] [Full Text] [PDF]

				A. Shifren and R. P. Mecham The stumbling block in lung repair of emphysema: elastic fiber assembly. Proceedings of the ATS, July 1, 2006; 3(5): 428 - 433. [Abstract] [Full Text] [PDF]

				D. M. Simon, M. C. Arikan, S. Srisuma, S. Bhattacharya, L. W. Tsai, E. P. Ingenito, F. Gonzalez, S. D. Shapiro, and T. J. Mariani Epithelial cell PPAR{gamma} contributes to normal lung maturation FASEB J, July 1, 2006; 20(9): 1507 - 1509. [Abstract] [Full Text] [PDF]

				M. Meyerson and D. Carbone Genomic and Proteomic Profiling of Lung Cancers: Lung Cancer Classification in the Age of Targeted Therapy J. Clin. Oncol., May 10, 2005; 23(14): 3219 - 3226. [Abstract] [Full Text] [PDF]

				B. H. Mecham, D. Z. Wetmore, Z. Szallasi, Y. Sadovsky, I. Kohane, and T. J. Mariani Increased measurement accuracy for sequence-verified microarray probes Physiol Genomics, August 11, 2004; 18(3): 308 - 315. [Abstract] [Full Text] [PDF]

				J. A. Whitsett, C. J. Bachurski, K. C. Barnes, P. A. Bunn Jr., L. M. Case, D. N. Cook, D. Crooks, M. W. Duncan, L. Dwyer-Nield, R. C. Elston, et al. Functional Genomics of Lung Disease Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2/S1): S1 - S81. [Full Text] [PDF]

				A. T. Kho, Q. Zhao, Z. Cai, A. J. Butte, J. Y.H. Kim, S. L. Pomeroy, D. H. Rowitch, and I. S. Kohane Conserved mechanisms across development and tumorigenesis revealed by a mouse development perspective of human cancers Genes & Dev., March 15, 2004; 18(6): 629 - 640. [Abstract] [Full Text] [PDF]

				M. A. Shultz, L. Zhang, Y.-Z. Gu, G. L. Baker, M. V. Fannuchi, A. M. Padua, W. A. Gurske, D. Morin, S. G. Penn, S. B. Jovanovich, et al. Gene Expression Analysis in Response to Lung Toxicants: I. Sequencing and Microarray Development Am. J. Respir. Cell Mol. Biol., March 1, 2004; 30(3): 296 - 310. [Abstract] [Full Text] [PDF]

				A. C. Borczuk, L. Gorenstein, K. L. Walter, A. A. Assaad, L. Wang, and C. A. Powell Non-Small-Cell Lung Cancer Molecular Signatures Recapitulate Lung Developmental Pathways Am. J. Pathol., November 1, 2003; 163(5): 1949 - 1960. [Abstract] [Full Text] [PDF]

				A E Bonner, W J Lemon, and M You Gene expression signatures identify novel regulatory pathways during murine lung development: implications for lung tumorigenesis J. Med. Genet., June 1, 2003; 40(6): 408 - 417. [Abstract] [Full Text] [PDF]

				L. Smith and A. Greenfield DNA microarrays and development Hum. Mol. Genet., April 2, 2003; 12(90001): R1 - 8. [Abstract] [Full Text] [PDF]

				R C Chambers Gene expression profiling: good housekeeping and a clean message Thorax, September 1, 2002; 57(9): 754 - 756. [Full Text]