help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jones, C. E.
Right arrow Articles by Chan, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jones, C. E.
Right arrow Articles by Chan, K.

Am. J. Respir. Cell Mol. Biol., Volume 26, Number 6, June, 2002 748-753

Interleukin-17 Stimulates the Expression of Interleukin-8, Growth-Related Oncogene-alpha , and Granulocyte-Colony-Stimulating Factor by Human Airway Epithelial Cells

Carol E. Jones and Katie Chan

Novartis Horsham Research Centre, West Sussex, United Kingdom

Interleukin (IL)-17 is a recently discovered cytokine, which is proposed to play a role in neutrophilic airway inflammation via the release of proinflammatory cytokines and chemokines. To evaluate the role of IL-17 in inflammatory protein production from the airway epithelium, we have analyzed the effects of IL-17 on primary human bronchial epithelial cells (HBECs). Using gene arrays, changes in gene expression in response to IL-17 stimulation were investigated and only IL-8, growth-related oncogene (Gro)alpha , and granulocyte colony-stimulating factor (G-CSF) were found to be upregulated. Secretion of IL-8, Groalpha , and G-CSF in response to IL-17 was measured in HBEC cell culture supernatants by enzyme-linked immunosorbent assay. Upregulation of Groalpha , IL-8, and G-CSF was observed to be 8-, 5-, and 8-fold, respectively, after 48 h stimulation with IL-17. When tested at equivalent concentrations, IL-17 was found to be 2- to 3-fold more potent than tumor necrosis factor (TNF)-alpha in stimulating release of Groalpha and G-CSF from HBECs. In addition, IL-17 was found to synergistically enhance TNF-alpha -induced production of IL-8, Groalpha , and G-CSF. It is proposed that IL-17 may play an important role in neutrophil recruitment via stimulating the release of IL-8, Groalpha , and G-CSF from airway epithelial cells.

Abbreviations: base pair(s), bp; enzyme-linked immunosorbent assay, ELISA; glyceraldehyde-3-phosphate dehydrogenase, GAPDH; granulocyte colony-stimulating factor, G-CSF; growth-related oncogene-alpha , Groalpha ; human bronchial epithelial cell, HBEC; interleukin, IL; reverse transcription- polymerase chain reaction, RT-PCR; tumor necrosis factor-alpha , TNF-alpha .




This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Freitas, J. C. Alves-Filho, T. Victoni, T. Secher, H. P. Lemos, F. Sonego, F. Q. Cunha, and B. Ryffel
IL-17 Receptor Signaling Is Required to Control Polymicrobial Sepsis
J. Immunol., June 15, 2009; 182(12): 7846 - 7854.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
N. Takahashi, I. Vanlaere, R. de Rycke, A. Cauwels, L. A.B Joosten, E. Lubberts, W. B. van den Berg, and C. Libert
IL-17 produced by Paneth cells drives TNF-induced shock
J. Exp. Med., August 4, 2008; 205(8): 1755 - 1761.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-Y. Kao, C. Kim, F. Huang, and R. Wu
Requirements for Two Proximal NF-{kappa}B Binding Sites and I{kappa}B-{zeta} in IL-17A-induced Human {beta}-Defensin 2 Expression by Conducting Airway Epithelium
J. Biol. Chem., May 30, 2008; 283(22): 15309 - 15318.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
V. Kim, T. J. Rogers, and G. J. Criner
New Concepts in the Pathobiology of Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, May 1, 2008; 5(4): 478 - 485.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. F. Y. Cheung, C. K. Wong, and C. W. K. Lam
Molecular Mechanisms of Cytokine and Chemokine Release from Eosinophils Activated by IL-17A, IL-17F, and IL-23: Implication for Th17 Lymphocytes-Mediated Allergic Inflammation
J. Immunol., April 15, 2008; 180(8): 5625 - 5635.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. J. Scriba, B. Kalsdorf, D.-A. Abrahams, F. Isaacs, J. Hofmeister, G. Black, H. Y. Hassan, R. J. Wilkinson, G. Walzl, S. J. Gelderbloem, et al.
Distinct, Specific IL-17- and IL-22-Producing CD4+ T Cell Subsets Contribute to the Human Anti-Mycobacterial Immune Response
J. Immunol., February 1, 2008; 180(3): 1962 - 1970.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Huang, C.-Y. Kao, S. Wachi, P. Thai, J. Ryu, and R. Wu
Requirement for Both JAK-Mediated PI3K Signaling and ACT1/TRAF6/TAK1-Dependent NF-{kappa}B Activation by IL-17A in Enhancing Cytokine Expression in Human Airway Epithelial Cells
J. Immunol., November 15, 2007; 179(10): 6504 - 6513.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
J. L. Curtis, C. M. Freeman, and J. C. Hogg
The Immunopathogenesis of Chronic Obstructive Pulmonary Disease: Insights from Recent Research
Proceedings of the ATS, October 1, 2007; 4(7): 512 - 521.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Wiehler and D. Proud
Interleukin-17A modulates human airway epithelial responses to human rhinovirus infection
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L505 - L515.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Dragon, M. S. Rahman, J. Yang, H. Unruh, A. J. Halayko, and A. S. Gounni
IL-17 enhances IL-1beta-mediated CXCL-8 release from human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L1023 - L1029.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Cruz, S. A. Khader, E. Torrado, A. Fraga, J. E. Pearl, J. Pedrosa, A. M. Cooper, and A. G. Castro
Cutting Edge: IFN-{gamma} Regulates the Induction and Expansion of IL-17-Producing CD4 T Cells during Mycobacterial Infection
J. Immunol., August 1, 2006; 177(3): 1416 - 1420.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Henness, E. van Thoor, Q. Ge, C. L. Armour, J. M. Hughes, and A. J. Ammit
IL-17A acts via p38 MAPK to increase stability of TNF-{alpha}-induced IL-8 mRNA in human ASM
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1283 - L1290.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C.-Y. Kao, F. Huang, Y. Chen, P. Thai, S. Wachi, C. Kim, L. Tam, and R. Wu
Up-Regulation of CC Chemokine Ligand 20 Expression in Human Airway Epithelium by IL-17 through a JAK-Independent but MEK/NF-{kappa}B-Dependent Signaling Pathway
J. Immunol., November 15, 2005; 175(10): 6676 - 6685.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. Ning, A. M. K. Choi, and C. Li
Carbon monoxide inhibits IL-17-induced IL-6 production through the MAPK pathway in human pulmonary epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L268 - L273.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. van den Berg, M. Kuiper, M. Snoek, W. Timens, D. S. Postma, H. M. Jansen, and R. Lutter
Interleukin-17 Induces Hyperresponsive Interleukin-8 and Interleukin-6 Production to Tumor Necrosis Factor-{alpha} in Structural Lung Cells
Am. J. Respir. Cell Mol. Biol., July 1, 2005; 33(1): 97 - 104.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. McAllister, A. Henry, J. L. Kreindler, P. J. Dubin, L. Ulrich, C. Steele, J. D. Finder, J. M. Pilewski, B. M. Carreno, S. J. Goldman, et al.
Role of IL-17A, IL-17F, and the IL-17 Receptor in Regulating Growth-Related Oncogene-{alpha} and Granulocyte Colony-Stimulating Factor in Bronchial Epithelium: Implications for Airway Inflammation in Cystic Fibrosis
J. Immunol., July 1, 2005; 175(1): 404 - 412.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A. Linden, M. Laan, and G. P. Anderson
Neutrophils, interleukin-17A and lung disease
Eur. Respir. J., January 1, 2005; 25(1): 159 - 172.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
P. J. Barnes
Mediators of Chronic Obstructive Pulmonary Disease
Pharmacol. Rev., December 1, 2004; 56(4): 515 - 548.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C.-Y. Kao, Y. Chen, P. Thai, S. Wachi, F. Huang, C. Kim, R. W. Harper, and R. Wu
IL-17 Markedly Up-Regulates {beta}-Defensin-2 Expression in Human Airway Epithelium via JAK and NF-{kappa}B Signaling Pathways
J. Immunol., September 1, 2004; 173(5): 3482 - 3491.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. J. Ruddy, F. Shen, J. B. Smith, A. Sharma, and S. L. Gaffen
Interleukin-17 regulates expression of the CXC chemokine LIX/CXCL5 in osteoblasts: implications for inflammation and neutrophil recruitment
J. Leukoc. Biol., July 1, 2004; 76(1): 135 - 144.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Ferretti, O. Bonneau, G. R. Dubois, C. E. Jones, and A. Trifilieff
IL-17, Produced by Lymphocytes and Neutrophils, Is Necessary for Lipopolysaccharide-Induced Airway Neutrophilia: IL-15 as a Possible Trigger
J. Immunol., February 15, 2003; 170(4): 2106 - 2112.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2002 American Thoracic Society. 		  Red In Translatin