help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bihl, M. P.
Right arrow Articles by Roth, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bihl, M. P.
Right arrow Articles by Roth, M.

Am. J. Respir. Cell Mol. Biol., Volume 27, Number 1, July, 2002 48-56

Identification of a Novel IL-6 Isoform Binding to the Endogenous IL-6 Receptor

Michel P. Bihl, Karl Heinimann, Jochen J. Rüdiger, Oliver Eickelberg, André P. Perruchoud, Michael Tamm, and Michael Roth

Pulmonary Cell Research and Human Genetics, Department of Research, Zentrum für Lehre und Forschung, Kantonsspital Basel, Basel, Switzerland; and Department of Pathology, University Hospital Yale, Yale University, New Haven, Connecticut

Interleukin (IL)-6 is a multifunctional cytokine showing a wide variety of biologic functions on various tissues. Extracellular IL-6 signals through heterohexameric complex formation with IL-6 receptor-alpha (IL-6Ralpha ) and IL-6 receptor-beta (IL-6Rbeta ). In analogy to cytokines IL-2 and IL-4, we investigated the expression of IL-6 splice variants in lung tissue and cultivated fibroblasts. In human lung specimens, four different IL-6 transcripts were characterized as follows: native IL-6; IL-6 missing either exon 2 (IL-6Delta 2), exon 4 (IL-6Delta 4), or missing both; and exons 2 and 4 (IL-6Delta 2,4). Only native IL-6 and IL-6Delta 4 encoded for proteins of ~ 26 and 17 kD, respectively. Although the overall structure and most functional sites of the IL-6Delta 4 protein were predicted to be maintained, IL-6Delta 4 was found to lack two amino acids necessary for IL-6/IL-6 homodimerization as well as two of the six amino acids required for interaction with IL-6Rbeta . Receptor mobility shift assays confirmed that the new isoform formed a stable complex with IL-6Ralpha ; however, no interaction with IL-6Rbeta was observed. Thus, IL-6Delta 4 is likely to compete with native IL-6 for IL-6Ralpha binding but fails to transmit IL-6Rbeta -mediated signaling.

Abbreviations: interleukin, IL; nuclear factor, NF.




This article has been cited by other articles:


Home page
 J Mol EndocrinolHome page
B. Jiao, X. Huang, C. B. Chan, L. Zhang, D. Wang, and C. H K Cheng
The co-existence of two growth hormone receptors in teleost fish and their differential signal transduction, tissue distribution and hormonal regulation of expression in seabream
J. Mol. Endocrinol., February 1, 2006; 36(1): 23 - 40.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
L. F. Zerbini, Y. Wang, J.-Y. Cho, and T. A Libermann
Constitutive Activation of Nuclear Factor {kappa}B p50/p65 and Fra-1 and JunD Is Essential for Deregulated Interleukin 6 Expression in Prostate Cancer
Cancer Res., May 1, 2003; 63(9): 2206 - 2215.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2002 American Thoracic Society. 		  CCM abstracts