help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kondo, M.
Right arrow Articles by Nagai, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kondo, M.
Right arrow Articles by Nagai, A.
American Journal of Respiratory Cell and Molecular Biology. Vol. 27, pp. 536-541, 2002
© 2002 American Thoracic Society
DOI: 10.1165/rcmb.4682

Interleukin-13 Induces Goblet Cell Differentiation in Primary Cell Culture from Guinea Pig Tracheal Epithelium

Mitsuko Kondo, Jun Tamaoki, Kiyoshi Takeyama, Junko Nakata and Atsushi Nagai

First Department of Medicine, Tokyo Women's Medical University, School of Medicine, Tokyo, Japan

Address correspondence to: Atsushi Nagai, M.D., First Department of Medicine, Tokyo Women's Medical University, School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. E-mail: anagai{at}chi.twmu.ac.jp

The Th2 cytokines, interleukin (IL)-4 and IL-13, bind to IL-4R{alpha}, and cause goblet cell metaplasia/hyperplasia with increased mucin expression in vivo. However, there is not enough evidence that these cytokines directly induce mucin production in vitro. In this study, primary epithelial cells from guinea pig trachea were cultured at an air–liquid interface, and immediately after achieving confluence at Day 7 they were treated with human recombinant IL-4 or IL-13 for 14 d. IL-13–treated cells consisted of a large number of fully mature goblet cells with a smaller number of ciliated cells. Secretory granules of the goblet cells were positive for both periodic acid-Schiff and toluidine blue, and showed exocytosis. By contrast, IL-4 failed to induce goblet cell differentiation. The electric resistances of IL-13–treated cells were lower than those of IL-4–treated cells and nontreated cells, suggesting leaky epithelia. MUC5AC protein level in cell lysates measured by ELISA was several-fold higher in IL-13–treated cells than in nontreated cells, whereas the level in IL-4–treated cells was not changed. These data suggest that human recombinant IL-13, but not IL-4, can induce differentiation into mature goblet cells that produce MUC5AC protein in guinea pig tracheal epithelial cells in vitro.

Abbreviations: epidermal growth factor, EGF • enzyme-linked immunosorbent assay, ELISA • interleukin, IL • ovalbumin, OVA • peridoc acid-Schiff, PAS • phosphate-buffered saline, PBS • transmission electron microscopy, TEM




This article has been cited by other articles:


Home page
 ThoraxHome page
A Bourdin, D Gras, I Vachier, and P Chanez
Upper airway {middle dot} 1: Allergic rhinitis and asthma: united disease through epithelial cells
Thorax, November 1, 2009; 64(11): 999 - 1004.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. Zhao, B. Maskrey, S. Balzar, K. Chibana, A. Mustovich, H. Hu, J. B. Trudeau, V. O'Donnell, and S. E. Wenzel
Interleukin-13-induced MUC5AC Is Regulated by 15-Lipoxygenase 1 Pathway in Human Bronchial Epithelial Cells
Am. J. Respir. Crit. Care Med., May 1, 2009; 179(9): 782 - 790.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
P-R. Burgel and J. A. Nadel
Epidermal growth factor receptor-mediated innate immune responses and their roles in airway diseases
Eur. Respir. J., October 1, 2008; 32(4): 1068 - 1081.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
I. Nakao, S. Kanaji, S. Ohta, H. Matsushita, K. Arima, N. Yuyama, M. Yamaya, K. Nakayama, H. Kubo, M. Watanabe, et al.
Identification of Pendrin as a Common Mediator for Mucus Production in Bronchial Asthma and Chronic Obstructive Pulmonary Disease
J. Immunol., May 1, 2008; 180(9): 6262 - 6269.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. Yamada, Y. Nishida, H. Tsutsumida, T. Hamada, M. Goto, M. Higashi, M. Nomoto, and S. Yonezawa
MUC1 Expression Is Regulated by DNA Methylation and Histone H3 Lysine 9 Modification in Cancer Cells
Cancer Res., April 15, 2008; 68(8): 2708 - 2716.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. N. Gomperts, L. J. Kim, S. A. Flaherty, and B. P. Hackett
IL-13 Regulates Cilia Loss and foxj1 Expression in Human Airway Epithelium
Am. J. Respir. Cell Mol. Biol., September 1, 2007; 37(3): 339 - 346.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T. Kiwamoto, Y. Ishii, Y. Morishima, K. Yoh, A. Maeda, K. Ishizaki, T. Iizuka, A. E. Hegab, Y. Matsuno, S. Homma, et al.
Transcription Factors T-bet and GATA-3 Regulate Development of Airway Remodeling
Am. J. Respir. Crit. Care Med., July 15, 2006; 174(2): 142 - 151.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. J. Holtzman, J. T. Battaile, and A. C. Patel
Immunogenetic Programs for Viral Induction of Mucous Cell Metaplasia
Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 29 - 39.
[Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. M. Casalino-Matsuda, M. E. Monzon, and R. M. Forteza
Epidermal Growth Factor Receptor Activation by Epidermal Growth Factor Mediates Oxidant-Induced Goblet Cell Metaplasia in Human Airway Epithelium
Am. J. Respir. Cell Mol. Biol., May 1, 2006; 34(5): 581 - 591.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
W. R. Henderson Jr., G. K. S. Chiang, Y.-t. Tien, and E. Y. Chi
Reversal of Allergen-induced Airway Remodeling by CysLT1 Receptor Blockade
Am. J. Respir. Crit. Care Med., April 1, 2006; 173(7): 718 - 728.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. K. Rowe and A. Pekosz
Bidirectional Virus Secretion and Nonciliated Cell Tropism following Andes Virus Infection of Primary Airway Epithelial Cell Cultures
J. Virol., February 1, 2006; 80(3): 1087 - 1097.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. Thai, Y. Chen, G. Dolganov, and R. Wu
Differential Regulation of MUC5AC/Muc5ac and hCLCA-1/mGob-5 Expression in Airway Epithelium
Am. J. Respir. Cell Mol. Biol., December 1, 2005; 33(6): 523 - 530.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. A. Kuperman, X. Huang, L. Nguyenvu, C. Holscher, F. Brombacher, and D. J. Erle
IL-4 Receptor Signaling in Clara Cells Is Required for Allergen-Induced Mucus Production
J. Immunol., September 15, 2005; 175(6): 3746 - 3752.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. V. Beum, H. Basma, D. R. Bastola, and P.-W. Cheng
Mucin biosynthesis: upregulation of core 2 {beta}1,6 N-acetylglucosaminyltransferase by retinoic acid and Th2 cytokines in a human airway epithelial cell line
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L116 - L124.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. Yoshisue, S. M. Puddicombe, S. J. Wilson, H. M. Haitchi, R. M. Powell, D. I. Wilson, A. Pandit, A. E. Berger, D. E. Davies, S. T. Holgate, et al.
Characterization of Ciliated Bronchial Epithelium 1, a Ciliated Cell-Associated Gene Induced During Mucociliary Differentiation
Am. J. Respir. Cell Mol. Biol., November 1, 2004; 31(5): 491 - 500.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. C. Atherton, G. Jones, and H. Danahay
IL-13-induced changes in the goblet cell density of human bronchial epithelial cell cultures: MAP kinase and phosphatidylinositol 3-kinase regulation
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L730 - L739.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Chen, P. Thai, Y.-H. Zhao, Y.-S. Ho, M. M. DeSouza, and R. Wu
Stimulation of Airway Mucin Gene Expression by Interleukin (IL)-17 through IL-6 Paracrine/Autocrine Loop
J. Biol. Chem., May 2, 2003; 278(19): 17036 - 17043.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2002 American Thoracic Society. 		  CCM abstracts