help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sarady, J. K.
Right arrow Articles by Choi, A. M. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sarady, J. K.
Right arrow Articles by Choi, A. M. K.
American Journal of Respiratory Cell and Molecular Biology. Vol. 27, pp. 739-745, 2002
© 2002 American Thoracic Society
DOI: 10.1165/rcmb.4816

Carbon Monoxide Modulates Endotoxin-Induced Production of Granulocyte Macrophage Colony-Stimulating Factor in Macrophages

Judit K. Sarady, Sherrie L. Otterbein, Fang Liu, Leo E. Otterbein and Augustine M. K. Choi

Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania

Address correspondence to: Leo E. Otterbein, Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh School of Medicine, 3459 5th Avenue, MUH628, Pittsburgh, PA 15213. E-mail: otterbeinl{at}msx.upmc.edu

The stress-inducible gene heme oxygenase-1 (HO-1) provides protection against oxidative stress. Although the mechanisms by which HO-1 exerts its cytoprotection are not clearly understood, it has been speculated that carbon monoxide (CO), a catalytic byproduct following heme catabolism by HO-1, may mediate cellular cytoprotection via its anti-inflammatory properties. Granulocyte macrophage colony-stimulating factor (GM-CSF) is a potent cytokine generated in response to bacterial endotoxin (lipopolysaccharide [LPS]) to stimulate proliferation, maturation, and effector functions of leukocytes, contributing to the proinflammatory responses to LPS. We hypothesized that HO-1 and/or CO could regulate the expression and production of GM-CSF. HO-1 overexpression, as well as exposure to a low concentration of CO, inhibited LPS-induced GM-CSF production in macrophages. Furthermore, CO inhibited LPS-induced GM-CSF induction via inhibition in the activation of the transcription factor NF-{kappa}B. CO inhibited LPS-induced activation of NF-{kappa}B, which has been shown to regulate GM-CSF transcription, by preventing the phosphorylation and degradation of the regulatory subunit I{kappa}B-{alpha}. These data raise the intriguing possibility that CO at low concentrations may play an important role in inflammatory disease states and thus has potential therapeutic implications.

Abbreviations: bronchoalveolar lavage fluid, BALF • carbon monoxide, CO • enzyme-linked immunosorbent assay, ELISA • electrophoretic mobility shift assay, EMSA • granulocyte macrophage–colony-stimulating factor, GM-CSF • heme oxygenase-1, HO-1 • lipopolysaccharide, LPS • mitogen-activated protein, MAP • nuclear factor-{kappa}B, NF-{kappa}B • parts per million, ppm • sodium dodecyl sulfate, SDS




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Nemzek, C. Fry, and O. Abatan
Low-dose carbon monoxide treatment attenuates early pulmonary neutrophil recruitment after acid aspiration
Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L644 - L653.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Z.-W. Xia, L.-Q. Xu, W.-W. Zhong, J.-J. Wei, N.-L. Li, J. Shao, Y.-Z. Li, S.-C. Yu, and Z.-L. Zhang
Heme Oxygenase-1 Attenuates Ovalbumin-Induced Airway Inflammation by Up-Regulation of Foxp3 T-Regulatory Cells, Interleukin-10, and Membrane-Bound Transforming Growth Factor- 1
Am. J. Pathol., December 1, 2007; 171(6): 1904 - 1914.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Lin, S. Weis, G. Yang, Y.-H. Weng, R. Helston, K. Rish, A. Smith, J. Bordner, T. Polte, F. Gaunitz, et al.
Heme Oxygenase-1 Protein Localizes to the Nucleus and Activates Transcription Factors Important in Oxidative Stress
J. Biol. Chem., July 13, 2007; 282(28): 20621 - 20633.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Haschemi, O. Wagner, R. Marculescu, B. Wegiel, S. C. Robson, N. Gagliani, D. Gallo, J.-F. Chen, F. H. Bach, and L. E. Otterbein
Cross-Regulation of Carbon Monoxide and the Adenosine A2a Receptor in Macrophages
J. Immunol., May 1, 2007; 178(9): 5921 - 5929.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. E. Fredenburgh, M. A. Perrella, and S. A. Mitsialis
The Role of Heme Oxygenase-1 in Pulmonary Disease
Am. J. Respir. Cell Mol. Biol., February 1, 2007; 36(2): 158 - 165.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
K. Nakahira, H. P. Kim, X. H. Geng, A. Nakao, X. Wang, N. Murase, P. F. Drain, X. Wang, M. Sasidhar, E. G. Nabel, et al.
Carbon monoxide differentially inhibits TLR signaling pathways by regulating ROS-induced trafficking of TLRs to lipid rafts
J. Exp. Med., October 2, 2006; 203(10): 2377 - 2389.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
W. A. Pryor, K. N. Houk, C. S. Foote, J. M. Fukuto, L. J. Ignarro, G. L. Squadrito, and K. J. A. Davies
Free radical biology and medicine: it's a gas, man!
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2006; 291(3): R491 - R511.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Y. Suh, Y. Jin, A.-K. Yi, X. M. Wang, and A. M. K. Choi
CCAAT/Enhancer-Binding Protein Mediates Carbon Monoxide-Induced Suppression of Cyclooxygenase-2
Am. J. Respir. Cell Mol. Biol., August 1, 2006; 35(2): 220 - 226.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Overhaus, B. A. Moore, J. E. Barbato, F. F. Behrendt, J. G. Doering, and A. J. Bauer
Biliverdin protects against polymicrobial sepsis by modulating inflammatory mediators
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G695 - G703.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. W. Ryter, J. Alam, and A. M. K. Choi
Heme Oxygenase-1/Carbon Monoxide: From Basic Science to Therapeutic Applications
Physiol Rev, April 1, 2006; 86(2): 583 - 650.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
X. M. Wang, H. P. Kim, R. Song, and A. M. K. Choi
Caveolin-1 Confers Antiinflammatory Effects in Murine Macrophages via the MKK3/p38 MAPK Pathway
Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 434 - 442.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
L. Wu and R. Wang
Carbon Monoxide: Endogenous Production, Physiological Functions, and Pharmacological Applications
Pharmacol. Rev., December 1, 2005; 57(4): 585 - 630.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. K. Sarady-Andrews, F. Liu, D. Gallo, A. Nakao, M. Overhaus, R. Ollinger, A. M. Choi, and L. E. Otterbein
Biliverdin administration protects against endotoxin-induced acute lung injury in rats
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1131 - L1137.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
Y. Jin and A. M. K. Choi
Cytoprotection of Heme Oxygenase-1/Carbon Monoxide in Lung Injury
Proceedings of the ATS, October 1, 2005; 2(3): 232 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Ghosh, M. R. Wilson, S. Choudhury, H. Yamamoto, M. E. Goddard, B. Falusi, N. Marczin, and M. Takata
Effects of inhaled carbon monoxide on acute lung injury in mice
Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1003 - L1009.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. H. Kapturczak, C. Wasserfall, T. Brusko, M. Campbell-Thompson, T. M. Ellis, M. A. Atkinson, and A. Agarwal
Heme Oxygenase-1 Modulates Early Inflammatory Responses: Evidence from the Heme Oxygenase-1-Deficient Mouse
Am. J. Pathol., September 1, 2004; 165(3): 1045 - 1053.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
I. A. Clark, L. M. Alleva, A. C. Mills, and W. B. Cowden
Pathogenesis of Malaria and Clinically Similar Conditions
Clin. Microbiol. Rev., July 1, 2004; 17(3): 509 - 539.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Guo, A. B. Stein, W.-J. Wu, W. Tan, X. Zhu, Q.-H. Li, B. Dawn, R. Motterlini, and R. Bolli
Administration of a CO-releasing molecule at the time of reperfusion reduces infarct size in vivo
Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1649 - H1653.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Kronke, V. N. Bochkov, J. Huber, F. Gruber, S. Bluml, A. Furnkranz, A. Kadl, B. R. Binder, and N. Leitinger
Oxidized Phospholipids Induce Expression of Human Heme Oxygenase-1 Involving Activation of cAMP-responsive Element-binding Protein
J. Biol. Chem., December 19, 2003; 278(51): 51006 - 51014.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Morse, S. E. Pischke, Z. Zhou, R. J. Davis, R. A. Flavell, T. Loop, S. L. Otterbein, L. E. Otterbein, and A. M. K. Choi
Suppression of Inflammatory Cytokine Production by Carbon Monoxide Involves the JNK Pathway and AP-1
J. Biol. Chem., September 26, 2003; 278(39): 36993 - 36998.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
F. A. D. T. G. Wagener, H.-D. Volk, D. Willis, N. G. Abraham, M. P. Soares, G. J. Adema, and C. G. Figdor
Different Faces of the Heme-Heme Oxygenase System in Inflammation
Pharmacol. Rev., September 1, 2003; 55(3): 551 - 571.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T.-S. Lee, H.-L. Tsai, and L.-Y. Chau
Induction of Heme Oxygenase-1 Expression in Murine Macrophages Is Essential for the Anti-inflammatory Effect of Low Dose 15-Deoxy-{Delta}12,14-prostaglandin J2
J. Biol. Chem., May 23, 2003; 278(21): 19325 - 19330.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2002 American Thoracic Society.