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Chlamydia pneumoniae Affect Surfactant Trafficking and Secretion Due to Changes of Type II Cell Cytoskeleton

Clinic of Neonatology, Campus Charité-Mitte, University Children's Hospital, Humboldt-University Berlin; and Department of Internal Medicine/Infectious Diseases, Charité, Medical School of Humboldt-University, Berlin, Germany

Address correspondence to: Heide Wissel, Ph.D., Clinic of Neonatology, CCM, University Hospital Charité, Schumannstrasse 20-21, 10098 Berlin, Germany. E-mail: hwissel{at}charite.de

Understanding the surfactant dysfunction by gram-negative bacteria pulmonary infection, the intracellular fate of Chlamydia pneumoniae (Cpn), its interaction with uptake, recycling, and secretion of surfactant and with the cytoskeleton of type II pneumocytes was investigated. Bacteria colocalized with surfactant protein (SP)-A–mediated endocytosed lipid and early endosomes (EEA1- and Rab5-positive) after 3 and 6 h of infection. No specific contact with late endosomes (Rab7- and M6PR-positive), lysosomal, or lamellar body markers (CD63, 3C9) was found after 12 h of infection. In Cpn-infected cells, SP-A–mediated lipid uptake was significantly increased. After SP-A–mediated lipid uptake followed by "re-secretion," 90% of the internalized lipid remained intracellularly. SP-A and lipid did strongly colocalize with early endosomes. Internalized SP-A cannot be resecreted rapidly to plasma membrane, and lipid is not transported toward late endosomes (Rab7- and M6PR-positive) or lamellar bodies (CD63- and 3C9-positive). These results indicate that increased surfactant internalization is caused by an inhibition in intracellular surfactant transport. Accumulation of SP-A–mediated lipid was associated with changes in ß-tubulin. Increases in surfactant secretion were associated with changes in F-actin. We postulate that Cpn infection of type II cells causes changes of the cytoskeleton, and that these effects are associated with alterations in intracellular transport and secretion of surfactant.

Abbreviations: Chlamydia pneumoniae, Cpn • inclusion-forming units, ifu • surfactant protein A, SP-A