This Article Full Text Full Text (PDF) All Versions of this Article: 2003-0178OCv1 30/6/880    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Melgert, B. N. Articles by Hylkema, M. N. Search for Related Content PubMed PubMed Citation Articles by Melgert, B. N. Articles by Hylkema, M. N.

Short-Term Smoke Exposure Attenuates Ovalbumin-Induced Airway Inflammation in Allergic Mice

Department of Pathology and Laboratory Medicine, and Department of Pulmonology, University Hospital Groningen, Groningen, The Netherlands

Address correspondence to: B. N. Melgert, Ph.D., Department of Pathology and Laboratory Medicine, University Hospital Groningen, P.O. Box 30.001, 9700 RB, Groningen, The Netherlands. E-mail: B.N.Melgert{at}path.azg.nl

Little is known about effects of smoking on airway inflammation in asthma. We tested the hypothesis that smoking enhances established airway inflammation in a mouse model of allergic asthma. C57Bl/6j mice were sensitized to ovalbumin (OVA) and challenged with OVA (OVA-mice) or sham-sensitized to phosphate-buffered saline (PBS) and challenged with PBS aerosols (PBS-mice) for 7 wk. At 4 wk, mice were additionally exposed to air (nonsmoking controls) or mainstream smoke for 3 wk. Using whole body plethysmography, we found OVA-induced bronchoconstriction to be significantly inhibited in smoking OVA-mice as compared with nonsmoking OVA-mice (1 ± 2% increase versus 22 ± 6% increase in enhanced pause, respectively). Smoking did not change airway hyperresponsiveness (AHR) to methacholine in PBS-mice, yet significantly attenuated AHR in OVA-mice 24 h after OVA challenge as compared with nonsmoking mice. This was accompanied by reduced eosinophil numbers in lung lavage fluid and tissue of smoking OVA-mice compared with nonsmoking OVA-mice. In contrast to our hypothesis, short-term smoking reduced responsiveness to OVA and methacholine in OVA-mice and decreased airway inflammation when compared with nonsmoking mice. This effect of smoking may be different for long-term smoking, in which remodeling effects of smoking can be expected to interrelate with remodeling changes caused by asthmatic disease.

Abbreviations: airway hyperresponsiveness, AHR • bronchoalveolar lavage, BAL • carbon monoxide, CO • enzyme-linked immunosorbent assay, ELISA • interleukin, IL • methacholine, MCh • ovalbumin, OVA • phosphate-buffered saline, PBS • enhanced pause, Penh

This article has been cited by other articles:

				L. J. Robays, E. A. Lanckacker, K. B. Moerloose, T. Maes, K. R. Bracke, G. G. Brusselle, G. F. Joos, and K. Y. Vermaelen Concomitant Inhalation of Cigarette Smoke and Aerosolized Protein Activates Airway Dendritic Cells and Induces Allergic Airway Inflammation in a TLR-Independent Way J. Immunol., August 15, 2009; 183(4): 2758 - 2766. [Abstract] [Full Text] [PDF]

				C.-H. Shih, T.-J. Huang, C.-M. Chen, Y.-L. Lin, and W.-C. Ko S-Petasin, the Main Sesquiterpene of Petasites formosanus, Inhibits Phosphodiesterase Activity and Suppresses Ovalbumin-induced Airway Hyperresponsiveness Evid. Based Complement. Altern. Med., July 29, 2009; (2009) nep088v1. [Abstract] [Full Text] [PDF]

				N. J. Trimble, F. M. Botelho, C. M. T. Bauer, R. Fattouh, and M. R. Stampfli Adjuvant and Anti-Inflammatory Properties of Cigarette Smoke in Murine Allergic Airway Inflammation Am. J. Respir. Cell Mol. Biol., January 1, 2009; 40(1): 38 - 46. [Abstract] [Full Text] [PDF]

				R. M. P. van Zundert, R. C. M. E. Engels, M. Kleinjan, and R. J. J. M. van den Eijnden An Integration of Parents' and Best Friends' Smoking, Smoking-Specific Cognitions, and Nicotine Dependence in Relation to Readiness to Quit Smoking: A Comparison between Adolescents with and without Asthma J. Pediatr. Psychol., September 1, 2008; 33(8): 821 - 832. [Abstract] [Full Text] [PDF]

				T. H. Thatcher, R. P. Benson, R. P. Phipps, and P. J. Sime High-dose but not low-dose mainstream cigarette smoke suppresses allergic airway inflammation by inhibiting T cell function Am J Physiol Lung Cell Mol Physiol, September 1, 2008; 295(3): L412 - L421. [Abstract] [Full Text] [PDF]

				E. Bathoorn, D-J. Slebos, D. S. Postma, G. H. Koeter, A. J. M. van Oosterhout, M. van der Toorn, H. M. Boezen, and H. A. M. Kerstjens Anti-inflammatory effects of inhaled carbon monoxide in patients with COPD: a pilot study Eur. Respir. J., December 1, 2007; 30(6): 1131 - 1137. [Abstract] [Full Text] [PDF]

				M. G. Min, D. J. Song, M. Miller, J. Y. Cho, S. McElwain, P. Ferguson, and D. H. Broide Coexposure to Environmental Tobacco Smoke Increases Levels of Allergen-Induced Airway Remodeling in Mice J. Immunol., April 15, 2007; 178(8): 5321 - 5328. [Abstract] [Full Text] [PDF]

				M. N. Hylkema, P. J. Sterk, W. I. de Boer, and D. S. Postma Tobacco use in relation to COPD and asthma Eur. Respir. J., March 1, 2007; 29(3): 438 - 445. [Abstract] [Full Text] [PDF]

				T. Sjaheim, J. Kongerud, O. Bjortuft, P. A. Drablos, D. Malterud, and T. S. Halstensen Reduced bronchial CD4+ T-cell density in smokers with occupational asthma Eur. Respir. J., December 1, 2006; 28(6): 1138 - 1144. [Abstract] [Full Text] [PDF]

				V. Phaybouth, S.-Z. Wang, J. A. Hutt, J. D. McDonald, K. S. Harrod, and E. G. Barrett Cigarette smoke suppresses Th1 cytokine production and increases RSV expression in a neonatal model Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L222 - L231. [Abstract] [Full Text] [PDF]

				C. C. Dong, X. J. Yin, J. Y. C. Ma, L. Millecchia, Z.-X. Wu, M. W. Barger, J. R. Roberts, J. M. Antonini, R. D. Dey, and J. K. H. Ma Effect of Diesel Exhaust Particles on Allergic Reactions and Airway Responsiveness in Ovalbumin-Sensitized Brown Norway Rats Toxicol. Sci., November 1, 2005; 88(1): 202 - 212. [Abstract] [Full Text] [PDF]

				C. S. Robbins, M. A. Pouladi, R. Fattouh, D. E. Dawe, N. Vujicic, C. D. Richards, M. Jordana, M. D. Inman, and M. R. Stampfli Mainstream Cigarette Smoke Exposure Attenuates Airway Immune Inflammatory Responses to Surrogate and Common Environmental Allergens in Mice, Despite Evidence of Increased Systemic Sensitization J. Immunol., September 1, 2005; 175(5): 2834 - 2842. [Abstract] [Full Text] [PDF]

				K. B. Moerloose, R. A. Pauwels, and G. F. Joos Short-Term Cigarette Smoke Exposure Enhances Allergic Airway Inflammation in Mice Am. J. Respir. Crit. Care Med., July 15, 2005; 172(2): 168 - 172. [Abstract] [Full Text] [PDF]

				M-R. Blanchet, E. Israel-Assayag, and Y. Cormier Modulation of airway inflammation and resistance in mice by a nicotinic receptor agonist Eur. Respir. J., July 1, 2005; 26(1): 21 - 27. [Abstract] [Full Text] [PDF]

				N.C. Thomson, R. Chaudhuri, and E. Livingston Asthma and cigarette smoking Eur. Respir. J., November 1, 2004; 24(5): 822 - 833. [Abstract] [Full Text] [PDF]