Published ahead of print on May 6, 2004, doi:10.1165/rcmb.2004-0080OC
© 2004 American Thoracic Society DOI: 10.1165/rcmb.2004-0080OC Proteome Comparison of Alveolar Macrophages with Monocytes Reveals Distinct Protein CharacteristicsDepartments of Pathology and Medicine, Dorothy M. Davis Heart and Lung Research Institutes, Ohio State University College of Medicine and Public Health, Columbus, Ohio Address correspondence to: Haifeng M. Wu, M.D., Assistant Professor and Director of Clinical Coagulation Laboratory, Department of Pathology, Ohio State University College of Medicine and Public Health, 288 Medical Research Facility, 420 West 12th Ave., Columbus, OH 43210. E-mail: wu-6{at}medctr.osu.edu Alveolar macrophages (AMs) are a subset of tissue macrophages situated in the alveolar milieu. Compared with their precursor blood monocytes, AMs exhibit distinct physiologic functions unique to their anatomic location. However, the molecular details that control monocyte differentiation into AMs remain unknown. This study employed a proteomic approach to define protein characteristics that distinguish AMs from monocytes. AMs and monocytes were obtained from six nonsmoking, healthy donors. Whole cell lysates from each donor's AMs and monocytes were analyzed by two-dimensional (2D) gel electrophoreses. The protein density for each protein spot in a 2D gel was compared between these two cell types. Proteins that demonstrated consistent level changes of greater than 2.5-fold in all six donors were subjected to tandem mass spectrometry for protein identity. Using this process, we revealed proteome changes in AMs that relate to their physiologic roles in proteolysis, actin reorganization, and cellular adaptation in the unique alveolar milieu. By comparison, blood monocytes displayed higher levels of the proteins involved in transcription, metabolism, inflammation, and in the control of proteolysis. These results provide new insights into the biology of mononuclear phagocytes and set a basis for future causality studies.
Abbreviations: two-dimensional, 2D • alveolar macrophages, AMs • bronchoalveolar lavage, BAL • chronic obstructive pulmonary disease, COPD • interleukin, IL • IL-1ß converting enzyme, ICE • immobilized pH gradient, IPG • mass spectrometry, MS • reactive oxygen species, ROS • selenium-binding proteins, SBP • superoxide dismutase, SOD • tripeptide peptidase I, TPP I This article has been cited by other articles:
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