Published ahead of print on July 8, 2004, doi:10.1165/rcmb.2004-0161OC
© 2004 American Thoracic Society DOI: 10.1165/rcmb.2004-0161OC Corticosteroid and Cytokines Synergistically Enhance Toll-Like Receptor 2 Expression in Respiratory Epithelial CellsDepartment of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo; Division of Allergy, National Center for Child Health and Development, Tokyo; Department of Otorhinolaryngology, Jikei Medical School, Tokyo; Departments of Microbiology and Pediatrics, Kochi Medical School, Kochi; Research Team for Allergy Transcriptome, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan Address correspondence to: Kenji Matsumoto, M.D., Ph.D., Department of Allergy and Immunology, National Research Institute for Child Health and Development, 3-35-31 Taishido, Setagaya-ku, Tokyo 154-8567, Japan. E-mail: kmatsumoto{at}nch.go.jp
Respiratory epithelial cells play important roles not only in host defense mechanisms, but also in inflammatory responses. Inhaled corticosteroids are widely used for the treatment of patients with inflammatory lung disorders, including asthma, chronic obstructive pulmonary disease, and sarcoidosis. Corticosteroids effectively reduce the production of inflammatory mediators, such as cytokines and chemokines. Although these molecules are also essential for host defense responses, there is no convincing evidence that inhaled corticosteroids increase susceptibility to lower respiratory tract infections. To test the involvement of Toll-like receptor (TLR) family molecules in this phenomenon, we examined the effects of various cytokines and corticosteroid on the expression of TLRs in human respiratory epithelial cells. Among the TLRs tested, TLR2 expression was significantly enhanced after stimulation with a combination of tumor necrosis factor
Abbreviations: bacterial lipoprotein, BLP dexamethasone, DEX fluorescence-activated cell sorter, FACS glucocorticoid receptor, GR glucocorticoid response element, GRE human ß-defensin2, hBD2 interferon, IFN interleukin, IL lipopolysaccharide, LPS mitogen-activated protein kinase, MAPK mean fluorescence intensity, MFI MAPK phosphatase-1, MKP-1 nuclear factor, NF normal human bronchial epithelial cells, NHBE nontypeable Haemophilus influenzae, NTHi pathogen-associated molecular patterns, PAMPs peripheral blood mononuclear cells, PBMC peptidoglycan, PGN Toll-like receptor, TLR tumor necrosis factor, TNF reverse transcriptasepolymerase chain reaction, RT-PCR This article has been cited by other articles:
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